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Enzyme Encapsulation in Layer-by-Layer Engineered Polymer Multilayer Capsules
Abstract
We report on the encapsulation of enzyme (catalase) by the controlled polymer multilayer coating of biocrystals, achieved by the sequential adsorption of oppositely charged polyelectrolytes onto enzyme crystal templates. An extremely high enzyme loading in each polymer capsule is obtained, and the activity of the encapsulated enzyme is preserved. The polymer-encapsulated enzyme is stable against protease degradation: The polymer-coated enzyme retains 100% of its activity after incubation for 100 min with protease, whereas uncoated, solubilized catalase loses more than 90% of its initial activity within 100 min under the same conditions. This simple, general, and versatile approach can potentially be applied for the encapsulation of various crystallized substances for catalysis and drug delivery applications.
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(1998) · Nature · doi:10.1038/32020 · Cited by 76
Cited by
Ariga, K., Hill, J. P., & Ji, Q. (2007) · Physical Chemistry Chemical Physics · doi:10.1039/b700410a · Cited by 1,256
Nanoencapsulation of phase change materials for advanced thermal energy storage systems
Shchukina, E. M., Graham, M., Zheng, Z., & Shchukin, D. G. (2018) · Chemical Society Reviews · doi:10.1039/c8cs00099a · Cited by 579
Self-assembly of nanoparticles at interfaces
Böker, A., He, J., Emrick, T., & Russell, T. P. (2007) · Soft Matter · doi:10.1039/b706609k · Cited by 566
van Dongen, S. F. M., de Hoog, H.-P. M., Peters, R. J. R. W., Nallani, M., Nolte, R. J. M., & van Hest, J. C. M. (2009) · Chemical Reviews · doi:10.1021/cr900072y · Cited by 386
pH-Responsive Properties of Multilayered Poly(l-lysine)/Hyaluronic Acid Surfaces
Burke, S. E., & Barrett, C. J. (2003) · Biomacromolecules · doi:10.1021/bm034184w · Cited by 255
Metadata
- Authors
- Caruso, F., Trau, D., Möhwald, H., & Renneberg, R.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2000-01-29
- Volume
- 16
- Issue
- 4
- Pages
- 1485-1488
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2036993803
- Cited by
- 533
- References
- 1
- FWCI
- 15.13
- Type
- journal article
- Language
- en
- Open access
- closed
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