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Metal-Enhanced Single-Molecule Fluorescence on Silver Particle Monomer and Dimer: Coupling Effect between Metal Particles
Open accessAbstract
We prepared silver particle dimers with single Cy5 molecules localized between coupled metal particles. The silver particles with a 20 nm diameter were chemically bound with single-stranded oligonucleotides. The dimers were formed by hybridization with double-length single-stranded oligonucleotides that contained single Cy5 molecules. The image analysis revealed that the single-molecule fluorescence was enhanced 7-fold on the metal monomer and 13-fold on the metal dimer relative to the free Cy5-labeled oligonucleotide in the absence of metal. The lifetimes were shortened on the silver monomers and further shortened on the silver dimers, demonstrating the near-field interaction mechanism of fluorophore with the metal substrate. Finite-difference time-domain (FDTD) calculations were employed to study the distribution of electric field near the metal monomer and dimer. The coupling effect of metal particle on the fluorescence enhancement was discussed.
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Nanostructures in Biodiagnostics
Rosi, N. L., & Mirkin, C. A. (2005) · Chemical Reviews · doi:10.1021/cr030067f · Cited by 4,603
Cited by
Guerrini, L., & Graham, D. (2012) · Chemical Society Reviews · doi:10.1039/c2cs35118h · Cited by 441
Schietinger, S., Barth, M., Aichele, T., & Benson, O. (2009) · Nano Letters · doi:10.1021/nl900384c · Cited by 398
Lee, J.-S., Shevchenko, E. V., & Talapin, D. V. (2008) · Journal of the American Chemical Society · doi:10.1021/ja802890f · Cited by 349
Metadata
- Authors
- Zhang, J., Fu, Y., Chowdhury, M. H., & Lakowicz, J. R.
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-06-20
- Volume
- 7
- Issue
- 7
- Pages
- 2101-2107
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W1998329957
- Cited by
- 387
- References
- 1
- FWCI
- 14.65
- Type
- journal article
- Language
- en
- Open access
- green
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