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Single-Molecule Kinetics and Super-Resolution Microscopy by Fluorescence Imaging of Transient Binding on DNA Origami
Abstract
DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications of these structures as functional biomaterials, the study of reaction kinetics and dynamic processes in real time and with high spatial resolution becomes increasingly important. We present a single-molecule assay for the study of binding and unbinding kinetics on DNA origami. We find that the kinetics of hybridization to single-stranded extensions on DNA origami is similar to isolated substrate-immobilized DNA with a slight position dependence on the origami. On the basis of the knowledge of the kinetics, we exploit reversible specific binding of labeled oligonucleotides to DNA nanostructures for PAINT (points accumulation for imaging in nanoscale topography) imaging with <30 nm resolution. The method is demonstrated for flat monomeric DNA structures as well as multimeric, ribbon-like DNA structures.
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Cited by
Super-resolution microscopy with DNA-PAINT
Schnitzbauer, J., Strauss, M. T., Schlichthaerle, T., Schueder, F., & Jungmann, R. (2017) · Nature Protocols · doi:10.1038/nprot.2017.024 · Cited by 1,047
Self-citationPrinciples and Applications of Nucleic Acid Strand Displacement Reactions
Simmel, F. C., Yurke, B., & Singh, H. R. (2019) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00580 · Cited by 792
Self-citationDNA nanotechnology from the test tube to the cell
Chen, Y.-J., Groves, B., Muscat, R. A., & Seelig, G. (2015) · Nature Nanotechnology · doi:10.1038/nnano.2015.195 · Cited by 594
Quantitative super-resolution imaging with qPAINT
Jungmann, R., Avendaño, M. S., Dai, M., Woehrstein, J. B., Agasti, S. S., Feiger, Z., Rodal, A., & Yin, P. (2016) · Nature Methods · doi:10.1038/nmeth.3804 · Cited by 420
Self-citationBeliveau, B. J., Boettiger, A. N., Avendaño, M. S., Jungmann, R., McCole, R. B., Joyce, E. F., Kim-Kiselak, C., Bantignies, F., Fonseka, C. Y., Erceg, J., et al. (2015) · Nature Communications · doi:10.1038/ncomms8147 · Cited by 384
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Metadata
- Authors
- Jungmann, R., Steinhauer, C., Scheible, M., Kuzyk, A., Tinnefeld, P., & Simmel, F. C.
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-10-19
- Volume
- 10
- Issue
- 11
- Pages
- 4756-4761
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W2109281145
- Cited by
- 903
- References
- 0
- FWCI
- 5.08
- Type
- journal article
- Language
- en
- Open access
- closed
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- Jungmann, R., Steinhauer, C., Scheible, M., Kuzyk, A., Tinnefeld, P., & Simmel, F. C. (2010). Single-Molecule Kinetics and Super-Resolution Microscopy by Fluorescence Imaging of Transient Binding on DNA Origami. Nano Letters. https://doi.org/10.1021/nl103427w
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