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Cited by 163

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Molecular Orientation Affects Localization Accuracy in Superresolution Far-Field Fluorescence Microscopy

Open access
Engelhardt, Johann, Keller, Jan, Hoyer, Patrick, Reuss, Matthias, Staudt, Thorsten, Hell, Stefan W.·2011·Nano Letters

Abstract

We investigate the cooperative effect of molecular tilt and defocus on fluorophore localization by centroid calculation in far-field superresolution microscopy based on stochastic single molecule switching. If tilt angle and defocus are unknown, the localization contains systematic errors up to about ±125 nm. When imaging rotation-impaired fluorophores of unknown random orientation, the average localization accuracy in three-dimensional samples is typically limited to about ±32 nm, restricting the attainable resolution accordingly.

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Metadata

Authors
Engelhardt, J., Keller, J., Hoyer, P., Reuss, M., Staudt, T., & Hell, S. W.
Venue
Nano Letters
Published
2010-12-06
Volume
11
Issue
1
Pages
209-213
ISSN
  • 1530-6984 (ISSN-L)
  • 1530-6992
OpenAlex
W2136683606
Cited by
163
References
1
FWCI
8.19
Type
journal article
Language
en
Open access
green

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