Single-molecule spectroscopy and imaging over the decades
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As of 2015, it has been 26 years since the first optical detection and spectroscopy of single molecules in condensed matter. This area of science has expanded far beyond the early low temperature studies in crystals to include single molecules in cells, polymers, and in solution. The early steps relied upon high-resolution spectroscopy of inhomogeneously broadened optical absorption profiles of molecular impurities in solids at low temperatures. Spectral fine structure arising directly from the position-dependent fluctuations of the number of molecules in resonance led to the attainment of the single-molecule limit in 1989 using frequency-modulation laser spectroscopy. In the early 1990s, a variety of fascinating physical effects were observed for individual molecules, including imaging of the light from single molecules as well as observations of spectral diffusion, optical switching and the ability to select different single molecules in the same focal volume simply by tuning the pumping laser frequency. In the room temperature regime, researchers showed that bursts of light from single molecules could be detected in solution, leading to imaging and microscopy by a variety of methods. Studies of single copies of the green fluorescent protein also uncovered surprises, especially the blinking and photoinduced recovery of emitters, which stimulated further development of photoswitchable fluorescent protein labels. All of these early steps provided important fundamentals underpinning the development of super-resolution microscopy based on single-molecule localization and active control of emitting concentration. Current thrust areas include extensions to three-dimensional imaging with high precision, orientational analysis of single molecules, and direct measurements of photodynamics and transport properties for single molecules trapped in solution by suppression of Brownian motion. Without question, a huge variety of studies of single molecules performed by many talented scientists all over the world have extended our knowledge of the nanoscale and many microscopic mechanisms previously hidden by ensemble averaging.
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Developments in experimental techniques such as ultrafast spectroscopy, single molecule spectroscopy, time-resolved microscopy and single particle imaging have enabled study of biological dynamics on increasingly small length and time scales, revealing processes necessary for living function that depend on a delicate interplay between quantum and classical physical effects.
“Recently, developments in experimental techniques such as ultrafast spectroscopy [6], single molecule spectroscopy [7 -11], timeresolved microscopy [12 -14] and single particle imaging [15 -18] have enabled us to study biological dynamics on increasingly small length and time scales, revealing a variety of processes necessary for the function of the living system that depend on a delicate interpl”
From The future of quantum biology (2018) · cites this paper as [7]
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Extending Single-Molecule Microscopy Using Optical Fourier Processing
Backer, A. S., & Moerner, W. E. (2014) · The Journal of Physical Chemistry B · doi:10.1021/jp501778z · Cited by 156 · see citing works
Self-citationDiffusion of low density lipoprotein-receptor complex on human fibroblasts
Barak, L. S., & Webb, W. W. (1982) · The Journal of Cell Biology · doi:10.1083/jcb.95.3.846 · Cited by 153 · see citing works
Optical modification of a single impurity molecule in a solid
Basché, T., & Moerner, W. E. (1992) · Nature · doi:10.1038/355335a0 · Cited by 153 · see citing works
Self-citationFueling protein–DNA interactions inside porous nanocontainers
Cissé, I. I., Okumuş, B., Joo, C., & Ha, T. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0610673104 · Cited by 150 · see citing works
Super-resolution fluorescence imaging with single molecules
Sahl, S. J., & Moerner, W. E. (2013) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2013.07.010 · Cited by 144 · see citing works
Self-citationWatching conformational- and photodynamics of single fluorescent proteins in solution
Goldsmith, R. H., & Moerner, W. E. (2010) · Nature Chemistry · doi:10.1038/nchem.545 · Cited by 142 · see citing works
Self-citationLew, M. D., Lee, S. F., Ptacin, J. L., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1114444108 · Cited by 140 · see citing works
Controlling Brownian motion of single protein molecules and single fluorophores in aqueous buffer
Cohen, A. E., & Moerner, W. E. (2008) · Optics Express · doi:10.1364/oe.16.006941 · Cited by 137 · see citing works
Self-citationElectrokinetic trapping at the one nanometer limit
Fields, A. P., & Cohen, A. E. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1103554108 · Cited by 136 · see citing works
Badieirostami, M., Lew, M. D., Thompson, M. A., et al. (2010) · Applied Physics Letters · doi:10.1063/1.3499652 · Cited by 132 · see citing works
Handbook of Single Molecule Fluorescence Spectroscopy
Gell, C., Brockwell, D. J., & Smith, A. D. (2006) · doi:10.1093/oso/9780198529422.001.0001 · Cited by 127 · see citing works
Small-Molecule Labeling of Live Cell Surfaces for Three-Dimensional Super-Resolution Microscopy
Lee, M. K., Rai, P., Williams, J. C., et al. (2014) · Journal of the American Chemical Society · doi:10.1021/ja508028h · Cited by 126 · see citing works
Structure and Dynamics in Solids As Probed by Optical Spectroscopy
Skinner, J. L., & Moerner, W. E. (1996) · The Journal of Physical Chemistry · doi:10.1021/jp9601328 · Cited by 124 · see citing works
Self-citationNear-Field Optical Spectroscopy of Individual Molecules in Solids
Moerner, W. E., Plakhotnik, T., Irngartinger, T., et al. (1994) · Physical Review Letters · doi:10.1103/physrevlett.73.2764 · Cited by 123 · see citing works
Self-citationSingle-molecule motions enable direct visualization of biomolecular interactions in solution
Wang, Q., & Moerner, W. E. (2014) · Nature Methods · doi:10.1038/nmeth.2882 · Cited by 121 · see citing works
Self-citationStochastic inhibitor release and binding from single-enzyme molecules
Gorris, H. H., Rissin, D. M., & Walt, D. R. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0705411104 · Cited by 121 · see citing works
Confocal three dimensional tracking of a single nanoparticle with concurrent spectroscopic readouts
Cang, H., Wong, C. M., Xu, C., et al. (2006) · Applied Physics Letters · doi:10.1063/1.2204652 · Cited by 120 · see citing works
Microscopy beyond the diffraction limit using actively controlled single molecules
Moerner, W. E. (2012) · Journal of Microscopy · doi:10.1111/j.1365-2818.2012.03600.x · Cited by 118 · see citing works
Self-citationThree-dimensional tracking of individual quantum dots
Lessard, G. A., Goodwin, P. M., & Werner, J. H. (2007) · Applied Physics Letters · doi:10.1063/1.2819074 · Cited by 115 · see citing works
Reilly, P. D., & Skinner, J. L. (1993) · Physical Review Letters · doi:10.1103/physrevlett.71.4257 · Cited by 114 · see citing works
Extending Microscopic Resolution with Single-Molecule Imaging and Active Control
Thompson, M. A., Lew, M. D., & Moerner, W. E. (2012) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-050511-102250 · Cited by 114 · see citing works
Self-citationTime-Resolved Three-Dimensional Molecular Tracking in Live Cells
Wells, N. P., Lessard, G. A., Goodwin, P. M., et al. (2010) · Nano Letters · doi:10.1021/nl103247v · Cited by 112 · see citing works
Rotational Motions of Macro- molecules by Single-Molecule Fluorescence Microscopy
Rosenberg, S. A., Quinlan, M. E., Forkey, J. N., et al. (2005) · Accounts of Chemical Research · doi:10.1021/ar040137k · Cited by 108 · see citing works
de Vries, H., & Wiersma, D. A. (1979) · The Journal of Chemical Physics · doi:10.1063/1.437411 · Cited by 106 · see citing works
Gahlmann, A., Ptacin, J. L., Grover, G., et al. (2013) · Nano Letters · doi:10.1021/nl304071h · Cited by 105 · see citing works
Quantum Dot Photon Statistics Measured by Three-Dimensional Particle Tracking
McHale, K., Berglund, A. J., & Mabuchi, H. (2007) · Nano Letters · doi:10.1021/nl0723376 · Cited by 105 · see citing works
Forkey, J., Quinlan, M., & Goldman, Y. (2005) · Biophysical Journal · doi:10.1529/biophysj.104.053470 · Cited by 105 · see citing works
Probing Single Biomolecules in Solution Using the Anti-Brownian Electrokinetic (ABEL) Trap
Wang, Q., Goldsmith, R. H., Jiang, Y., Bockenhauer, S. D., & Moerner, W. E. (2012) · Accounts of Chemical Research · doi:10.1021/ar200304t · Cited by 104 · see citing works
Self-citationSingle-Molecule Identification of Quenched and Unquenched States of LHCII
Schlau‐Cohen, G. S., Yang, H., Krüger, T. P. J., Xu, P., Gwizdala, M., van Grondelle, R., Croce, R., & Moerner, W. E. (2015) · The Journal of Physical Chemistry Letters · doi:10.1021/acs.jpclett.5b00034 · Cited by 103 · see citing works
Self-citationBasché, T., Ambrose, W. P., & Moerner, W. E. (1992) · Journal of the Optical Society of America B · doi:10.1364/josab.9.000829 · Cited by 102 · see citing works
Self-citationHigh-Resolution Optical Spectroscopy of Single Molecules in Solids
Moerner, W. E. (1996) · Accounts of Chemical Research · doi:10.1021/ar950245u · Cited by 101 · see citing works
Self-citationEffects of surface tethering on protein folding mechanisms
Friedel, M., Baumketner, A., & Shea, J. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601210103 · Cited by 100 · see citing works
Real‐Time Nanomicroscopy via Three‐Dimensional Single‐Particle Tracking
Katayama, Y., Burkacky, O., Meyer, M. S., et al. (2009) · ChemPhysChem · doi:10.1002/cphc.200900436 · Cited by 96 · see citing works
Baddeley, D., Cannell, M. B., & Soeller, C. (2011) · Nano Research · doi:10.1007/s12274-011-0115-z · Cited by 94 · see citing works
Perillo, E. P., Liu, Y., Huynh, K. T., Liu, C., Chou, C., Hung, M., Yeh, H., & Dunn, A. K. (2015) · Nature Communications · doi:10.1038/ncomms8874 · Cited by 92 · see citing works
Conformational Dynamics of Single G Protein-Coupled Receptors in Solution
Bockenhauer, S. D., Fürstenberg, A., Xiao, Y., Kobilka, B. K., & Moerner, W. E. (2011) · The Journal of Physical Chemistry B · doi:10.1021/jp204843r · Cited by 92 · see citing works
Self-citationMoerner, W. E. (2015) · Reviews of Modern Physics · doi:10.1103/revmodphys.87.1183 · Cited by 91 · see citing works
Self-citationGuiding a confocal microscope by single fluorescent nanoparticles
Cang, H., Xu, C., Montiel, D., et al. (2007) · Optics Letters · doi:10.1364/ol.32.002729 · Cited by 87 · see citing works
Single-molecule spectroscopy reveals photosynthetic LH2 complexes switch between emissive states
Schlau‐Cohen, G. S., Wang, Q., Southall, J., Cogdell, R. J., & Moerner, W. E. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1310222110 · Cited by 86 · see citing works
Self-citationVibronic Spectroscopy of Individual Molecules in Solids
Myers, A. B., Tchénio, P., Zgierski, M. Z., et al. (1994) · The Journal of Physical Chemistry · doi:10.1021/j100092a001 · Cited by 86 · see citing works
Three-Dimensional Tracking of Single Fluorescent Particles with Submillisecond Temporal Resolution
Juette, M. F., & Bewersdorf, J. (2010) · Nano Letters · doi:10.1021/nl1028792 · Cited by 86 · see citing works
Kador, L., Horne, D. E., & Moerner, W. E. (1990) · The Journal of Physical Chemistry · doi:10.1021/j100367a011 · Cited by 86 · see citing works
Self-citationWang, Q., & Moerner, W. E. (2011) · ACS Nano · doi:10.1021/nn2014968 · Cited by 85 · see citing works
Self-citationReilly, P. D., & Skinner, J. L. (1995) · The Journal of Chemical Physics · doi:10.1063/1.468886 · Cited by 80 · see citing works
Backer, A. S., Backlund, M. P., von Diezmann, L., et al. (2014) · Applied Physics Letters · doi:10.1063/1.4876440 · Cited by 79 · see citing works
Principal-components analysis of shape fluctuations of single DNA molecules
Cohen, A. E., & Moerner, W. E. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0610396104 · Cited by 78 · see citing works
Self-citationOptische Spektroskopie von einzelnen Dotierungsmolekülen in Festkörpern
Moerner, W. E., & Basché, T. (1993) · Angewandte Chemie · doi:10.1002/ange.19931050406 · Cited by 77 · see citing works
Self-citationResolving Single-Molecule Assembled Patterns with Superresolution Blink-Microscopy
Cordes, T., Strackharn, M., Ståhl, S., et al. (2009) · Nano Letters · doi:10.1021/nl903730r · Cited by 76 · see citing works
Sensing cooperativity in ATP hydrolysis for single multisubunit enzymes in solution
Jiang, Y., Douglas, N. R., Conley, N. R., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1112244108 · Cited by 74 · see citing works
Grover, G., DeLuca, K. F., Quirin, S., et al. (2012) · Optics Express · doi:10.1364/oe.20.026681 · Cited by 74 · see citing works
Statistical Fine Structure of Inhomogeneously Broadened Absorption Lines
Moerner, W. E., & Carter, T. P. (1987) · Physical Review Letters · doi:10.1103/physrevlett.59.2705 · Cited by 73 · see citing works
Self-citationOptimal strategy for trapping single fluorescent molecules in solution using the ABEL trap
Wang, Q., & Moerner, W. E. (2009) · Applied Physics B · doi:10.1007/s00340-009-3843-y · Cited by 71 · see citing works
Self-citationTchénio, P., Myers, A. B., & Moerner, W. E. (1993) · Chemical Physics Letters · doi:10.1016/0009-2614(93)85140-j · Cited by 68 · see citing works
Self-citationSingle-molecule orientation measurements with a quadrated pupil
Backer, A. S., Backlund, M. P., Lew, M. D., et al. (2013) · Optics Letters · doi:10.1364/ol.38.001521 · Cited by 68 · see citing works
Near-isotropic 3D optical nanoscopy with photon-limited chromophores
Tang, J., Akerboom, J., Vaziri, A., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1004899107 · Cited by 67 · see citing works
Mass Action at the Single-Molecule Level
Shon, M. J., & Cohen, A. E. (2012) · Journal of the American Chemical Society · doi:10.1021/ja3062425 · Cited by 62 · see citing works
Wang, Q., & Moerner, W. E. (2012) · The Journal of Physical Chemistry B · doi:10.1021/jp308949d · Cited by 62 · see citing works
Self-citationCan single-photon processes provide useful materials for frequency-domain optical storage?
Moerner, W. E., & Levenson, M. D. (1985) · Journal of the Optical Society of America B · doi:10.1364/josab.2.000915 · Cited by 61 · see citing works
Self-citationMechanisms of non-photochemical hole-burning in organic glasses
Hayes, J. M., & Small, G. J. (1978) · Chemical Physics Letters · doi:10.1016/0009-2614(78)85255-5 · Cited by 60 · see citing works
Lee, M. K., Williams, J. C., Twieg, R. J., et al. (2012) · Chemical Science · doi:10.1039/c2sc21074f · Cited by 60 · see citing works
Lew, M. D., & Moerner, W. E. (2014) · Nano Letters · doi:10.1021/nl502914k · Cited by 60 · see citing works
Self-citationMolecules and Methods for Super-Resolution Imaging
Thompson, M. A., Biteen, J. S., Lord, S. J., et al. (2010) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(10)75002-3 · Cited by 59 · see citing works
Ondrus, A. E., Lee, H. D., Iwanaga, S., et al. (2012) · Chemistry & Biology · doi:10.1016/j.chembiol.2012.05.021 · Cited by 57 · see citing works
Redox cycling and kinetic analysis of single molecules of solution-phase nitrite reductase
Goldsmith, R. H., Tabares, L. C., Kostrz, D. N., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1113572108 · Cited by 56 · see citing works
Dispersed fluorescence spectra of single molecules of pentacene in p-terphenyl
Tchénio, P., Myers, A. B., & Moerner, W. E. (1993) · The Journal of Physical Chemistry · doi:10.1021/j100113a003 · Cited by 56 · see citing works
Self-citationMoerner, W. E., & Ambrose, W. P. (1991) · Physical Review Letters · doi:10.1103/physrevlett.66.1376 · Cited by 55 · see citing works
Self-citationSingle molecule polarization spectroscopy: pentacene in p-terphenyl
Güttler, F., Croci, M., Renn, A., et al. (1996) · Chemical Physics · doi:10.1016/0301-0104(96)00250-9 · Cited by 52 · see citing works
Dissecting pigment architecture of individual photosynthetic antenna complexes in solution
Wang, Q., & Moerner, W. E. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1514027112 · Cited by 48 · see citing works
Self-citationSingle-Molecule and Superresolution Imaging in Live Bacteria CellsPreprint
Biteen, J. S., & Moerner, W. E. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a000448 · Cited by 48 · see citing works
Self-citationDavid, Baddeley, ', M., et al. (2011) · 纳米研究：英文版 · Cited by 48 · see citing works
Using photon statistics to boost microscopy resolution
Michalet, X., & Weiss, S. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0600808103 · Cited by 47 · see citing works
The anti-Brownian electrophoretic trap (ABEL trap): fabrication and software
Cohen, A. E., & Moerner, W. E. (2005) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.598689 · Cited by 45 · see citing works
Self-citationSingle Molecules and Nanotechnology
Rigler, R. W., & Vogel, H. (2007) · Springer series in biophysics · doi:10.1007/978-3-540-73924-1 · Cited by 44 · see citing works
Friedrich, J., & Haarer, D. (1986) · Physics and chemistry of materials with low-dimensional structures. Series C, Molecular structures · doi:10.1007/978-94-009-4650-7_4 · Cited by 44 · see citing works
Electron Spin Resonance of Nitrogen-Vacancy Defects Embedded in Single Nanodiamonds in an ABEL Trap
Kayci, M., Chang, H., & Rađenović, A. (2014) · Nano Letters · doi:10.1021/nl5023964 · Cited by 40 · see citing works
II Optical Spectroscopy of Single Molecules in Solids
Orrit, M., Bernard, J., Brown, R., & Lounis, B. (1996) · Progress in optics · doi:10.1016/s0079-6638(08)70528-6 · Cited by 39 · see citing works
Carter, T. P., Manavi, M., & Moerner, W. E. (1988) · The Journal of Chemical Physics · doi:10.1063/1.455123 · Cited by 35 · see citing works
Self-citationOptimal tracking of a Brownian particle
Fields, A. P., & Cohen, A. E. (2012) · Optics Express · doi:10.1364/oe.20.022585 · Cited by 33 · see citing works
Single Molecule Spectroscopy in Shpol'skii Matrices
Plakhotnik, T., Moerner, W. E., Irngartinger, T., et al. (1994) · CHIMIA International Journal for Chemistry · doi:10.2533/chimia.1994.31 · Cited by 32 · see citing works
Self-citationSchlau‐Cohen, G. S., Bockenhauer, S. D., Wang, Q., & Moerner, W. E. (2014) · Chemical Science · doi:10.1039/c4sc00582a · Cited by 27 · see citing works
Self-citationMcHale, K., & Mabuchi, H. (2009) · Journal of the American Chemical Society · doi:10.1021/ja906979j · Cited by 26 · see citing works
Rebane, K. K. (1994) · Chemical Physics · doi:10.1016/0301-0104(94)00298-3 · Cited by 22 · see citing works
Single-Molecule Spectroscopy and Quantum Optics in Solids
Moerner, W. E., Dickson, R. M., & Norris, D. J. (1998) · Advances in atomic, molecular, and optical physics · doi:10.1016/s1049-250x(08)60053-1 · Cited by 21 · see citing works
Self-citationCarter, T. P., Horne, D. E., & Moerner, W. E. (1988) · Chemical Physics Letters · doi:10.1016/0009-2614(88)80078-2 · Cited by 18 · see citing works
Self-citationPhoto-Induced Conformational Flexibility in Single Solution-Phase Peridinin-Chlorophyll-Proteins
Bockenhauer, S. D., & Moerner, W. E. (2013) · The Journal of Physical Chemistry A · doi:10.1021/jp405790a · Cited by 18 · see citing works
Self-citationThree-dimensional anti-Brownian electrokinetic trapping of a single nanoparticle in solution
King, J. K., Canfield, B. K., & Davis, L. M. (2013) · Applied Physics Letters · doi:10.1063/1.4816325 · Cited by 13 · see citing works
Controlled bimolecular collisions allow sub-diffraction limited microscopy of lipid vesicles
Mei, E., Gao, F., & Hochstrasser, R. M. (2006) · Physical Chemistry Chemical Physics · doi:10.1039/b601670g · Cited by 12 · see citing works
Setup for single-particle orbit tracking: artifacts and corrections
Ernst, D., Hain, S., & Köhler, J. R. (2012) · Journal of the Optical Society of America A · doi:10.1364/josaa.29.001277 · Cited by 10 · see citing works
(2012) · Springer eBooks · doi:10.1007/978-3-642-16712-6_100309 · Cited by 10 · see citing works
Single-Molecule Photocontrol and Nanoscopy
Lew, M. D., Lee, S. F., Thompson, M. A., et al. (2012) · Springer series on fluorescence · doi:10.1007/4243_2011_37 · Cited by 3 · see citing works
Flagged first, then by citations
Research works printed but not linked: 9
- [14]Zander C Enderlein J Keller RA Single-Molecule Detection in Solution: Methods and Applications Wiley-VCH Berlin 2002
- [28]Moerner WE Topics in Current Physics 44; Persistent Spectral Hole-Burning: Science and Applications Springer Berlin 1988
- [63]Elson EL Magde D Biopolymers 1974 13 1 27 4818131
- [65]Magde D Webb WW Elson EL Biopolymers 1978 17 361 367 4818131
- [68]Rigler R Widengren J 1990 Proceedings of Bioscience 3 180 183
- [83]Moerner WE Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium 138 Proceedings Graslund A Rigler R Widengren J Springer-Verlag Berlin 2009 25 60
- [84]Rigler R Orrit M Basche T Single Molecule Spectroscopy, Nobel Conference Lectures Springer-Verlag Berlin 2001
- [85]Gräslund A Rigler R Widengren J Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium 138 Proceedings Springer-Verlag Berlin 2009
- [202]Schlau-Cohen GS Wang Q Southall J Cogdell RJ Moerner WE Proc Natl Acad Sci U S A 2013 110 10899 10903 23776245 PMC3704035
Cited by
Sorted by citations
Marais, A., Adams, B., Ringsmuth, A. K., Ferretti, M., Gruber, J. M., Hendrikx, R., Schuld, M., Smith, S. L., Sinayskiy, I., Krüger, T. P. J., et al. (2018) · Journal of The Royal Society Interface · doi:10.1098/rsif.2018.0640 · Cited by 274 · see citing works
Labeling and Single-Molecule Methods To Monitor G Protein-Coupled Receptor Dynamics
Tian, H., Fürstenberg, A., & Huber, T. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00084 · Cited by 114 · see citing works
- Authors
- Moerner, W. E., Shechtman, Y., & Wang, Q.
- Affiliations
- Stanford University (US)
- Corresponding author
- Moerner, W. E.
- Venue
- Faraday Discussions
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2015
- Volume
- 184
- Pages
- 9-36
- ISSN
- 1359-6640 (ISSN-L)
- 1364-5498
- PMC
- PMC4782608
- OpenAlex
- W2119662414
- Cited by
- 103
- References
- 198
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 10 · 2023: 14 · 2022: 9 · 2021: 9 · 2020: 16 · 2019: 12 · 2018: 12 · 2017: 8 · 2016: 4
- FWCI
- 0.80
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Faraday Discussions (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM085437, R01GM086196)
- Basic Energy Sciences (DE-FG02-07ER15892)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
- Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
- Keywords
- single-molecule spectroscopy, single-molecule imaging, spectral diffusion, optical switching, single-molecule fluorescence microscopy, photoswitchable fluorescent proteins, super-resolution microscopy, single-molecule localization, molecular orientation, photodynamics, single-molecule transport
- MeSH terms
- Spectrometry, Fluorescence, Temperature, History, 20th Century, History, 21st Century
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