The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Mapping Intracellular Temperature Using Green Fluorescent Protein
Open accessAbstract
Heat is of fundamental importance in many cellular processes such as cell metabolism, cell division and gene expression. (1-3) Accurate and noninvasive monitoring of temperature changes in individual cells could thus help clarify intricate cellular processes and develop new applications in biology and medicine. Here we report the use of green fluorescent proteins (GFP) as thermal nanoprobes suited for intracellular temperature mapping. Temperature probing is achieved by monitoring the fluorescence polarization anisotropy of GFP. The method is tested on GFP-transfected HeLa and U-87 MG cancer cell lines where we monitored the heat delivery by photothermal heating of gold nanorods surrounding the cells. A spatial resolution of 300 nm and a temperature accuracy of about 0.4 °C are achieved. Benefiting from its full compatibility with widely used GFP-transfected cells, this approach provides a noninvasive tool for fundamental and applied research in areas ranging from molecular biology to therapeutic and diagnostic studies.
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Temperature Sensing Using Fluorescent Nanothermometers
Vetrone, F., Naccache, R., Zamarrón, A., Juarranz de la Fuente, A., Sanz-Rodríguez, F., Martinez Maestro, L., Martín Rodriguez, E., Jaque, D., García Solé, J., & Capobianco, J. A. (2010) · ACS Nano · doi:10.1021/nn100244a · Cited by 1,505
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Luminescent probes and sensors for temperature
Wang, X.-D., Wolfbeis, O. S., & Meier, R. J. (2013) · Chemical Society Reviews · doi:10.1039/c3cs60102a · Cited by 1,647
Optical temperature sensing of up-conversion luminescent materials: Fundamentals and progress
Zhao, Y., Wang, X., Zhang, Y., Li, Y., & Yao, X. (2020) · Journal of Alloys and Compounds · doi:10.1016/j.jallcom.2019.152691 · Cited by 380
Ke, G., Wang, C., Ge, Y., Zheng, N., Zhu, Z., & Yang, C. J. (2012) · Journal of the American Chemical Society · doi:10.1021/ja3082439 · Cited by 196
Petrini, G., Moreva, E., Bernardi, E., Traina, P., Tomagra, G., Carabelli, V., Degiovanni, I. P., & Genovese, M. (2020) · Advanced Quantum Technologies · doi:10.1002/qute.202000066 · Cited by 63
Metadata
- Authors
- Donner, J. S., Thompson, S. A., Kreuzer, M. P., Baffou, G., & Quidant, R.
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-03-12
- Volume
- 12
- Issue
- 4
- Pages
- 2107-2111
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W2036328014
- Cited by
- 441
- References
- 30
- FWCI
- 12.79
- Type
- journal article
- Language
- en
- Open access
- green
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