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Fractionation Protocol for the Isolation of Polypeptides from Plant Biomass
Abstract
A fractionation protocol for the isolation of a highly purified polypeptide fraction from plant biomass is described. The procedure dereplicates ubiquitous substance classes known to interfere with bioassays often used in natural product-based drug discovery programs. The protocol involves pre-extraction with dichloromethane, extraction with ethanol (50%), removal of tannins with polyamide, removal of low-molecular-weight components with size-exclusion chromatography over Sephadex G-10, and final removal of salts and polysaccharides with solid-phase extraction using reversed-phase cartridges. The method has been applied to the aerial parts of Viola arvensis, resulting in the isolation of a peptide fraction that on further separation yielded a novel 29-residue macrocyclic polypeptide named varv peptide A, cyclo(-TCVGGTCNTPGCSCSWPVCTRNGLPVCGE-).
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Citations
Cited by
Colgrave, M. L., & Craik, D. J. (2004) · Biochemistry · doi:10.1021/bi049711q · Cited by 580
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Trabi, M., & Craik, D. J. (2002) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(02)02057-1 · Cited by 255
New drugs from marine microbes: The tide is turning
Newman, D. J., & Hill, R. T. (2006) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-006-0115-2 · Cited by 144
Characterization of cysteine proteases in Malian medicinal plants
Bah, S., Paulsen, B. S., Diallo, D., & Johansen, H. T. (2006) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2006.03.008 · Cited by 19
Metadata
- Authors
- Claeson, P., Göransson, U., Johansson, S., Luijendijk, T., & Bohlin, L.
- Venue
- Journal of Natural Products
- Publisher
- American Chemical Society (ACS)
- Published
- 1998-01-01
- Volume
- 61
- Issue
- 1
- Pages
- 77-81
- ISSN
- 0163-3864 (ISSN-L)
- 1520-6025
- OpenAlex
- W2056072225
- Cited by
- 163
- References
- 0
- FWCI
- 7.61
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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