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Use of Ionic Liquids to Increase the Yield and Enzyme Stability in the β-Galactosidase Catalysed Synthesis of N-Acetyllactosamine
Abstract
The use of ionic liquids as alternative solvents for enzyme catalysis was investigated. β-Galactosidase from Bacillus circulans catalyses the synthesis of N -acetyllactosamine starting from lactose and N -acetylglucosamine in a transglycoslyation reaction. The addition of 25% v/v of 1,3-di-methyl-imidazol-methyl sulfate [MMIM] [MeSO 4 ] as a water-miscible ionic liquid suppresses the secondary hydrolysis of the formed product, resulting in doubling the yield to almost 60%. The enzyme can be reused several times after ultrafiltration of the reaction mixture without loss of activity. Results of different amounts of ionic liquids in the reaction medium on the thermostability of the galactosidase as well as on oxidoreductases are presented as well.
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Citations
Cited by
Pârvulescu, V. I., & Hardacre, C. (2007) · Chemical Reviews · doi:10.1021/cr050948h · Cited by 2,377
Applications of Ionic Liquids in Carbohydrate Chemistry: A Window of Opportunities
El Seoud, O. A., Koschella, A., Fidale, L. C., Dorn, S., & Heinze, T. (2007) · Biomacromolecules · doi:10.1021/bm070062i · Cited by 668
Advances on biomass pretreatment using ionic liquids: An overview
Tadesse, H., & Luque, R. (2011) · Energy & Environmental Science · doi:10.1039/c0ee00667j · Cited by 434
Methods for stabilizing and activating enzymes in ionic liquids—a review
Zhao, H. (2010) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.2375 · Cited by 351
Ionic liquids in enzymatic catalysis and biochemical methods of analysis: Capabilities and prospects
Muginova, S. V., Galimova, A. Z., Polyakov, A. E., & Shekhovtsova, T. N. (2010) · Journal of Analytical Chemistry · doi:10.1134/s1061934810040027 · Cited by 36
Metadata
- Authors
- Kaftzik, N., Wasserscheid, P., & Kragl, U.
- Venue
- Organic Process Research & Development
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-06-27
- Volume
- 6
- Issue
- 4
- Pages
- 553-557
- ISSN
- 1083-6160 (ISSN-L)
- 1520-586X
- OpenAlex
- W2021287806
- Cited by
- 193
- References
- 0
- FWCI
- 2.27
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kaftzik, N., Wasserscheid, P., & Kragl, U. (2002). Use of Ionic Liquids to Increase the Yield and Enzyme Stability in the β-Galactosidase Catalysed Synthesis of N-Acetyllactosamine. Organic Process Research & Development. https://doi.org/10.1021/op0255231
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