The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A New Strategy for Identification of N-Glycosylated Proteins and Unambiguous Assignment of Their Glycosylation Sites Using HILIC Enrichment and Partial Deglycosylation
Abstract
Characterization of glycoproteins using mass spectrometry ranges from determination of carbohydrate-protein linkages to the full characterization of all glycan structures attached to each glycosylation site. In a novel approach to identify N-glycosylation sites in complex biological samples, we performed an enrichment of glycosylated peptides through hydrophilic interaction liquid chromatography (HILIC) followed by partial deglycosylation using a combination of endo-beta-N-acetylglucosaminidases (EC 3.2.1.96). After hydrolysis with these enzymes, a single N-acetylglucosamine (GlcNAc) residue remains linked to the asparagine residue. The removal of the major part of the glycan simplifies the MS/MS fragment ion spectra of glycopeptides, while the remaining GlcNAc residue enables unambiguous assignment of the glycosylation site together with the amino acid sequence. We first tested our approach on a mixture of known glycoproteins, and subsequently the method was applied to samples of human plasma obtained by lectin chromatography followed by 1D gel-electrophoresis for determination of 62 glycosylation sites in 37 glycoproteins.
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Cites
Anderson, N. L., & Anderson, N. G. (2002) · Molecular & Cellular Proteomics · doi:10.1074/mcp.r200007-mcp200 · Cited by 4,414
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Interpreting the protein language using proteomics
Jensen, O. N. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1939 · Cited by 502
Self-citationMass spectrometry approaches to glycomic and glycoproteomic analyses
Ruhaak, L. R., Xu, G., Li, Q., Goonatilleke, E., & Lebrilla, C. B. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00732 · Cited by 395
Zhao, Y., & Jensen, O. N. (2009) · PROTEOMICS · doi:10.1002/pmic.200900398 · Cited by 384
Self-citationHigh-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
Determination of glycosylation sites and site-specific heterogeneity in glycoproteins
An, H. J., Froehlich, J. W., & Lebrilla, C. B. (2009) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2009.07.022 · Cited by 280
Gu, Y., Liu, Y., Fu, L., Zhai, L., Zhu, J., Han, Y., Jiang, Y., Zhang, Y., Zhang, P., Jiang, Z., et al. (2019) · Nature Medicine · doi:10.1038/s41591-018-0309-y · Cited by 266
Mucin‐type O‐glycosylation – putting the pieces together
Jensen, P. H., Kolarich, D., & Packer, N. H. (2010) · The FEBS Journal · doi:10.1111/j.1742-4658.2009.07429.x · Cited by 252
Glycoproteomics: A Balance between High-Throughput and In-Depth Analysis
Yang, Y., Franc, V., & Heck, A. J. R. (2017) · Trends in Biotechnology · doi:10.1016/j.tibtech.2017.04.010 · Cited by 122
Ahmad Izaham, A. R., Ang, C.-S., Nie, S., Bird, L. E., Williamson, N. A., & Scott, N. E. (2021) · Journal of Proteome Research · doi:10.1021/acs.jproteome.0c00565 · Cited by 62
Hoffman, M. D., Walsh, G. M., Rogalski, J. C., & Kast, J. (2009) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m800230-mcp200 · Cited by 41
Metadata
- Authors
- Hägglund, P., Bunkenborg, J., Elortza, F., Jensen, O. N., & Roepstorff, P.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-04-10
- Volume
- 3
- Issue
- 3
- Pages
- 556-566
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2066144238
- Cited by
- 460
- References
- 1
- FWCI
- 6.67
- Type
- journal article
- Language
- en
- Open access
- closed
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