Glycoproteomics: A Balance between High-Throughput and In-Depth Analysis
Open accessAbstract
Glycoproteomics is an important subdiscipline of proteomics. Mass spectrometry (MS)-based glycoproteomics has relied so far on two levels of analysis, targeting either the released glycans or digested glycopeptides. However, limitations of these approaches, caused by the vast structural microheterogeneity that glycoproteins can exhibit, have become evident. Therefore, concomitant methods enabling deep characterization of all discrete glycoforms are essential to extend our functional understanding of the glycoproteome. Here, we discuss recent progress, particularly in protein-centric approaches. High-resolution native MS appears to be especially promising in revealing the glycoform profile of glycoproteins. We propose that systematically integrating MS data acquired at the glycan, glycopeptide, and glycoprotein levels best enhances our understanding of the glycoproteome.
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CZE separation has frequently been used to analyze glycosylated peptides and glycans, and CZE via TDMS has been used for glycosylation characterization.
“CZE separation has frequently been used to analyze glycosylated peptides and glycans49,57,80,81 and CZE separation via TDMS was used for glycosylation characterization82.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [82]
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Native mass spectrometry: a bridge between interactomics and structural biology
Heck, A. J. R. (2008) · Nature Methods · doi:10.1038/nmeth.1265 · Cited by 766 · see citing works
Self-citationNative Mass Spectrometry: What is in the Name?
Leney, A. C., & Heck, A. J. R. (2017) · Journal of the American Society for Mass Spectrometry · doi:10.1007/s13361-016-1545-3 · Cited by 602 · see citing works
Self-citationDecoding protein modifications using top-down mass spectrometry
Siuti, N., & Kelleher, N. L. (2007) · Nature Methods · doi:10.1038/nmeth1097 · Cited by 503 · see citing works
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Advances in mass spectrometry driven O-glycoproteomics
Levery, S. B., Steentoft, C., Halim, A., Narimatsu, Y., Clausen, H., & Vakhrushev, S. Y. (2015) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2014.09.026 · Cited by 111 · see citing works
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High-Resolution Native Mass Spectrometry
Tamara, S., den Boer, M. A., & Heck, A. J. R. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00212 · Cited by 511 · see citing works
Self-citationRecent trends of capillary electrophoresis‐mass spectrometry in proteomics research
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Glycoengineering in CHO cells: advances in systems biology
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Capillary zone electrophoresis-mass spectrometry for bottom-up proteomics
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- Authors
- Yang, Y., Franc, V., & Heck, A. J. R.
- Affiliations
- Utrecht University (NL)
- Corresponding author
- Heck, Albert J.R.
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2017-07
- Volume
- 35
- Issue
- 7
- Pages
- 598-609
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W2615718202
- Cited by
- 122
- References
- 6
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 8 · 2023: 9 · 2022: 10 · 2021: 22 · 2020: 25 · 2019: 20 · 2018: 21
- FWCI
- 1.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Trends in biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, other-oa)
- Utrecht University Repository (Utrecht University) (submittedVersion)
- Funders
- Netherlands Organization for Scientific Research
- European Union (317371)
- EU’s Horizon 2020 Research and Innovation Programme for MSMed (686547)
- NWO TOP-Punt (718.015.003)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- glycoproteomics, glycopeptides, glycan profiling, glycoforms, native mass spectrometry, high-resolution mass spectrometry
- MeSH terms
- Animals, Glycopeptides, Glycoproteins, Humans, Mass Spectrometry, Proteomics
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