The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Mass Spectrometric Characterization of Human Histone H3: A Bird's Eye View
Abstract
The modification of H3 in asynchronous HeLa cells was profiled using Top Down Mass Spectrometry. A broad distribution of species differing by 14 Da and containing less than 3% unmodified protein was observed for all three variants. Species of up to +168 Da were observed for H3.1, and fragmentation of all species by Electron Capture Dissociation (ECD) revealed ∼5% methylation of K4 and ∼50% dimethylation of K9. K14 and K23 were major sites of acetylation. H3.3 was slightly hypermodified with the apex of the distribution shifted by ∼+14 Da compared to H3.1. H3.1 (50% and 15%) from colchicine-treated cells was monophosphorylated and diphosphorylated, respectively, with equivalent modification of S10 and S28. Keywords: H3 • histone code • histone • Fourier transform mass spectrometry (FTMS) • electron capture dissociation (ECD) • chromatin • post-translational modification (PTM) • acetylation • methylation • phosphorylation
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Cites
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins
Lachner, M., O'Carroll, D., Rea, S., Mechtler, K., & Jenuwein, T. (2001) · Nature · doi:10.1038/35065132 · Cited by 2,868
Cited by
Reversal of Histone Lysine Trimethylation by the JMJD2 Family of Histone Demethylases
Whetstine, J. R., Nottke, A., Lan, F., Huarte, M., Smolikov, S., Chen, Z., Spooner, E., Li, E., Zhang, G., Colaiacovo, M., et al. (2006) · Cell · doi:10.1016/j.cell.2006.03.028 · Cited by 1,040
Progress in Top-Down Proteomics and the Analysis of Proteoforms
Toby, T. K., Fornelli, L., & Kelleher, N. L. (2016) · Annual Review of Analytical Chemistry · doi:10.1146/annurev-anchem-071015-041550 · Cited by 576
Self-citationDecoding protein modifications using top-down mass spectrometry
Siuti, N., & Kelleher, N. L. (2007) · Nature Methods · doi:10.1038/nmeth1097 · Cited by 503
Self-citationCross-regulation of histone modifications
Latham, J. A., & Dent, S. Y. R. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1307 · Cited by 405
Quantitative Proteomic Analysis of Histone Modifications
Huang, H., Lin, S., Garcia, B. A., & Zhao, Y. (2015) · Chemical Reviews · doi:10.1021/cr500491u · Cited by 370
Metadata
- Authors
- Thomas, C. E., Kelleher, N. L., & Mizzen, C. A.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-01-11
- Volume
- 5
- Issue
- 2
- Pages
- 240-247
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2003632039
- Cited by
- 190
- References
- 1
- FWCI
- 9.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- Thomas, C. E., Kelleher, N. L., & Mizzen, C. A. (2006). Mass Spectrometric Characterization of Human Histone H3: A Bird's Eye View. Journal of Proteome Research. https://doi.org/10.1021/pr050266a
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