The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Effects of Modified Digestion Schemes on the Identification of Proteins from Complex Mixtures
Open accessAbstract
In shotgun proteomics, a complex protein mixture is digested to peptides, separated, and identified by microcapillary liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). In this technology, complete protein digestion is often assumed. We show that, to the contrary, modifications to a standard digestion protocol demonstrate large, reproducible improvements in protein identification, a result consistent with digestion being a limiting factor in the efficiency of protein identification.
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Citations
Cites
Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS
Gerber, S. A., Rush, J., Stemman, O., Kirschner, M. W., & Gygi, S. P. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0832254100 · Cited by 1,733
Cited by
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491
Assigning Significance to Peptides Identified by Tandem Mass Spectrometry Using Decoy Databases
Käll, L., Storey, J. D., MacCoss, M. J., & Noble, W. S. (2008) · Journal of Proteome Research · doi:10.1021/pr700600n · Cited by 607
Self-citationAccurate and Sensitive Peptide Identification with Mascot Percolator
Brosch, M., Yu, L., Hubbard, T., & Choudhary, J. (2009) · Journal of Proteome Research · doi:10.1021/pr800982s · Cited by 428
Glatter, T., Ludwig, C., Ahrné, E., Aebersold, R., Heck, A. J. R., & Schmidt, A. (2012) · Journal of Proteome Research · doi:10.1021/pr300273g · Cited by 364
Hoshi, Y., Uchida, Y., Tachikawa, M., Inoue, T., Ohtsuki, S., & Terasaki, T. (2013) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.23575 · Cited by 254
Optimization of Mass Spectrometry-Compatible Surfactants for Shotgun Proteomics
Chen, E. I., Cociorva, D., Norris, J. L., & Yates, J. R. (2007) · Journal of Proteome Research · doi:10.1021/pr060682a · Cited by 218
Yildiz, P., & Ozcan, S. (2023) · Talanta · doi:10.1016/j.talanta.2023.124878 · Cited by 2
Metadata
- Authors
- Klammer, A. A., & MacCoss, M. J.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-02-11
- Volume
- 5
- Issue
- 3
- Pages
- 695-700
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W1982782877
- Cited by
- 164
- References
- 1
- FWCI
- 5.77
- Type
- journal article
- Language
- en
- Open access
- green
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