The DOI matches its Crossref record; 69 of the 69 references it cites were found in a registry or an index.
Identification of Proteins Associated with the Pseudomonas aeruginosa Biofilm Extracellular Matrix
Abstract
Biofilms are surface-associated bacteria that are embedded in a matrix of self-produced polymeric substances (EPSs). The EPS is composed of nucleic acids, polysaccharides, lipids, and proteins. While polysaccharide components have been well studied, the protein content of the matrix is largely unknown. Here we conducted a comprehensive proteomic study to identify proteins associated with the biofilm matrix of Pseudomonas aeruginosa PAO1 (the matrix proteome). This analysis revealed that approximately 30% of the identified matrix proteins were outer membrane proteins, which are also typically found in outer membrane vesicles (OMVs). Electron microscopic inspection confirmed the presence of large amounts of OMVs within the biofilm matrix, supporting previous notions that OMVs are abundant constituents of P. aeruginosa biofilms. Our results demonstrate that while some proteins associated with the P. aeruginosa matrix are derived from secreted proteins and lysed cells, the large majority of the matrix proteins originate from OMVs. Furthermore, we demonstrate that the protein content of planktonic and biofilm OMVs is surprisingly different and may reflect the different physiological states of planktonic and sessile cells.
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Cited by
Biofilms: an emergent form of bacterial life
Flemming, H.-C., Wingender, J., Szewzyk, U., Steinberg, P., Rice, S. A., & Kjelleberg, S. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.94 · Cited by 5,979
Types and origins of bacterial membrane vesicles
Toyofuku, M., Nomura, N., & Eberl, L. (2019) · Nature Reviews Microbiology · doi:10.1038/s41579-018-0112-2 · Cited by 1,303
Self-citationExplosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms
Turnbull, L., Toyofuku, M., Hynen, A. L., Kurosawa, M., Pessi, G., Petty, N. K., Osvath, S. R., Cárcamo-Oyarce, G., Gloag, E. S., Shimoni, R., et al. (2016) · Nature Communications · doi:10.1038/ncomms11220 · Cited by 709
Self-citationMicrobial Biofilms in the Food Industry—A Comprehensive Review
Carrascosa, C., Raheem, D., Ramos, F., Saraiva, A., & Raposo, A. (2021) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph18042014 · Cited by 459
Gram-negative and Gram-positive bacterial extracellular vesicles
Kim, J. H., Lee, J., Park, J., & Gho, Y. S. (2015) · Seminars in Cell & Developmental Biology · doi:10.1016/j.semcdb.2015.02.006 · Cited by 454
Metadata
- Authors
- Toyofuku, M., Roschitzki, B., Riedel, K., & Eberl, L.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-09-26
- Volume
- 11
- Issue
- 10
- Pages
- 4906-4915
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2094149145
- Cited by
- 262
- References
- 69
- FWCI
- 4.88
- Type
- journal article
- Language
- en
- Open access
- closed
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