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Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250
Abstract
An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G‐250 and R‐250. The new method is based on addition of 20 % v/v methanol and higher concentrations of ammonium sulfate to the staining solution previously described [1]. The method combines the advantage of much shorter staining time with high sensitivity, a clear background not requiring destaining, stepwise staining, and stable fixation after staining. The method has been applied to staining of polyacrylamide gels after sodium dodecyl sulfate‐electrophoresis and isoelectric focusing in carrier ampholyte‐generated pH gradients.
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Citations
Cited by
Methods for samples preparation in proteomic research
Bodzon-Kulakowska, A., Bierczynska-Krzysik, A., Dylag, T., Drabik, A., Suder, P., Noga, M., Jarzebinska, J., & Silberring, J. (2007) · Journal of Chromatography B · doi:10.1016/j.jchromb.2006.10.040 · Cited by 254
Chernin, L. S., Winson, M. K., Thompson, J. M., Haran, S., Bycroft, B. W., Chet, I., Williams, P., & Stewart, G. S. A. B. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.17.4435-4441.1998 · Cited by 238
Brosch, G., Ransom, R., Lechner, T., Walton, J. D., & Loidl, P. (1995) · The Plant Cell · doi:10.1105/tpc.7.11.1941 · Cited by 204
The cell wall subproteome of Listeria monocytogenes
Schaumburg, J., Diekmann, O., Hagendorff, P., Bergmann, S., Rohde, M., Hammerschmidt, S., Jänsch, L., Wehland, J., & Kärst, U. (2004) · PROTEOMICS · doi:10.1002/pmic.200400928 · Cited by 199
Alterations in the determinants of diastolic suction during pacing tachycardia
Bell, S. P., Nyland, L., Tischler, M. D., McNabb, M., Granzier, H., & LeWinter, M. M. (2000) · Circulation Research · doi:10.1161/01.res.87.3.235 · Cited by 190
Metadata
- Authors
- Neuhoff, V., Arold, N., Taube, D., & Ehrhardt, W.
- Venue
- ELECTROPHORESIS
- Publisher
- Wiley
- Published
- 1988-01
- Volume
- 9
- Issue
- 6
- Pages
- 255-262
- ISSN
- 0173-0835 (ISSN-L)
- 1522-2683
- OpenAlex
- W1964313477
- Cited by
- 2,676
- References
- 0
- FWCI
- 13.52
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250. https://app.cochat.ai/papers/10.1002/elps.1150090603
- The paper
- Neuhoff, V., Arold, N., Taube, D., & Ehrhardt, W. (1988). Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250. ELECTROPHORESIS. https://doi.org/10.1002/elps.1150090603
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