The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Phosphoproteome Analysis of Fission Yeast
Abstract
Phosphorylation is a key regulator of many events in eukaryotic cells. The acquisition of large-scale phosphorylation data sets from model organisms can pinpoint conserved regulatory inputs and reveal kinase-substrate relationships. Here, we provide the first large-scale phosphorylation analysis of the fission yeast, Schizosaccharomyces pombe. Protein from thiabendazole-treated cells was separated by preparative SDS-PAGE and digested with trypsin. The resulting peptides were subjected to either IMAC or TiO2 phosphopeptide enrichment methods and then analyzed by LC-MS/MS using an LTQ-Orbitrap mass spectrometer. In total, 2887 distinct phosphorylation sites were identified from 1194 proteins with an estimated false-discovery rate of <0.5% at the peptide level. A comparison of the two different enrichment methods is presented, supporting the finding that they are complementary. Finally, phosphorylation sites were examined for phosphorylation-specific motifs and evolutionary conservation. These analyses revealed both motifs and specific phosphorylation events identified in S. pombe were conserved and predicted novel phosphorylation in mammals.
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Cites
OrthoMCL: Identification of Ortholog Groups for Eukaryotic Genomes
Li, L., Stoeckert, C. J., & Roos, D. S. (2003) · Genome Research · doi:10.1101/gr.1224503 · Cited by 5,707
Elias, J. E., & Gygi, S. P. (2007) · Nature Methods · doi:10.1038/nmeth1019 · Cited by 3,925
Self-citationATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage
Matsuoka, S., Ballif, B. A., Smogorzewska, A., McDonald, E. R., Hurov, K. E., Luo, J., Bakalarski, C. E., Zhao, Z., Solimini, N., Lerenthal, Y., et al. (2007) · Science · doi:10.1126/science.1140321 · Cited by 3,075
Self-citationBeausoleil, S. A., Villén, J., Gerber, S. A., Rush, J., & Gygi, S. P. (2006) · Nature Biotechnology · doi:10.1038/nbt1240 · Cited by 1,577
Self-citationLarge-scale phosphorylation analysis of mouse liver
Villén, J., Beausoleil, S. A., Gerber, S. A., & Gygi, S. P. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0609836104 · Cited by 730
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A quantitative atlas of mitotic phosphorylation
Dephoure, N., Zhou, C., Villén, J., Beausoleil, S. A., Bakalarski, C. E., Elledge, S. J., & Gygi, S. P. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0805139105 · Cited by 1,608
Self-citationThe SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
Villén, J., & Gygi, S. P. (2008) · Nature Protocols · doi:10.1038/nprot.2008.150 · Cited by 629
Self-citationAnalytical strategies for phosphoproteomics
Thingholm, T. E., Jensen, O. N., & Larsen, M. R. (2009) · PROTEOMICS · doi:10.1002/pmic.200800454 · Cited by 477
Zhao, Y., & Jensen, O. N. (2009) · PROTEOMICS · doi:10.1002/pmic.200900398 · Cited by 384
Phosphoproteome Analysis of Drosophila melanogaster Embryos
Zhai, B., Villén, J., Beausoleil, S. A., Mintseris, J., & Gygi, S. P. (2008) · Journal of Proteome Research · doi:10.1021/pr700696a · Cited by 270
Self-citationSoulard, A., Cremonesi, A., Moes, S., Schütz, F., Jenö, P., & Hall, M. N. (2010) · Molecular Biology of the Cell · doi:10.1091/mbc.e10-03-0182 · Cited by 262
Evolution of protein phosphatases in plants and animals
Moorhead, G. B. G., De Wever, V., Templeton, G., & Kerk, D. (2009) · Biochemical Journal · doi:10.1042/bj20081986 · Cited by 254
Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species
Beltrao, P., Trinidad, J. C., Fiedler, D., Roguev, A., Lim, W. A., Shokat, K. M., Burlingame, A. L., & Krogan, N. J. (2009) · PLoS Biology · doi:10.1371/journal.pbio.1000134 · Cited by 188
Metadata
- Authors
- Wilson-Grady, J. T., Villén, J., & Gygi, S. P.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-02-08
- Volume
- 7
- Issue
- 3
- Pages
- 1088-1097
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W1966062268
- Cited by
- 180
- References
- 5
- FWCI
- 10.46
- Type
- journal article
- Language
- en
- Open access
- closed
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