The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Analytical strategies for phosphoproteomics
Open accessAbstract
Protein phosphorylation is a key regulator of cellular signaling pathways. It is involved in most cellular events in which the complex interplay between protein kinases and protein phosphatases strictly controls biological processes such as proliferation, differentiation, and apoptosis. Defective or altered signaling pathways often result in abnormalities leading to various diseases, emphasizing the importance of understanding protein phosphorylation. Phosphorylation is a transient modification, and phosphoproteins are often very low abundant. Consequently, phosphoproteome analysis requires highly sensitive and specific strategies. Today, most phosphoproteomic studies are conducted by mass spectrometric strategies in combination with phospho‐specific enrichment methods. This review presents an overview of different analytical strategies for the characterization of phosphoproteins. Emphasis will be on the affinity methods utilized specifically for phosphoprotein and phosphopeptide enrichment prior to MS analysis, and on recent applications of these methods in cell biological applications.
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“Others introduce the tag at the protein level (isotope-coded affinity tags, ICAT [125]) or peptide level (isobaric peptide tags for relative and absolute quantifi-”
BackgroundRoss, P. L., Huang, Y. N., Marchese, J. N., Williamson, B., Parker, K., Hattan, S., Khainovski, N., Pillai, S., Dey, S., Daniels, S., et al. (2004) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m400129-mcp200 · Cited by 4,401
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Proteomic Analysis of in Vivo Phosphorylated Synaptic Proteins
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Cited by
Catch me if you can: Mass spectrometry‐based phosphoproteomics and quantification strategies
Eyrich, B., Sickmann, A., & Zahedi, R. P. (2011) · PROTEOMICS · doi:10.1002/pmic.201000489 · Cited by 104
A hydrophilic magnetic MOF for the consecutive enrichment of exosomes and exosomal phosphopeptides
Zhang, N., Sun, N., & Deng, C. (2020) · Chemical Communications · doi:10.1039/d0cc06147f · Cited by 69
Mass spectrometric analysis of protein tyrosine nitration in aging and neurodegenerative diseases
Yeo, W., Kim, Y. J., Kabir, M. H., Kang, J. W., & Kim, K. P. (2015) · Mass Spectrometry Reviews · doi:10.1002/mas.21429 · Cited by 61
Metadata
- Authors
- Thingholm, T. E., Jensen, O. N., & Larsen, M. R.
- Venue
- PROTEOMICS
- Publisher
- Wiley
- Published
- 2009-03
- Volume
- 9
- Issue
- 6
- Pages
- 1451-1468
- ISSN
- 1615-9853 (ISSN-L)
- 1615-9861
- OpenAlex
- W2170736411
- Cited by
- 477
- References
- 7
- FWCI
- 23.67
- Type
- journal article
- Language
- en
- Open access
- bronze
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