The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction
Abstract
The synthesis of living entities in the laboratory is a standing challenge that calls for innovative approaches. Using a cell-free transcription-translation system as a molecular programming platform, we show that the bacteriophage T7, encoded by a 40 kbp DNA program composed of about 60 genes, can be entirely synthesized from its genomic DNA in a test tube reaction. More than a billion infectious bacteriophages T7 per milliliter of reaction are produced after a few hours of incubation. The replication of the genomic DNA occurs concurrently with phage gene expression, protein synthesis, and viral assembly. The demonstration that genome-sized viral DNA can be expressed in a test tube, recapitulating the entire chain of information processing including the replication of the DNA instructions, opens new possibilities to program and to study complex biochemical systems in vitro.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A vesicle bioreactor as a step toward an artificial cell assembly
Noireaux, V., & Libchaber, A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0408236101 · Cited by 1,152
Self-citationExpression of a cascading genetic network within liposomes
Ishikawa, K., Sato, K., Shima, Y., Urabe, I., & Yomo, T. (2004) · FEBS Letters · doi:10.1016/j.febslet.2004.09.046 · Cited by 215
Cited by
Cell-free gene expression: an expanded repertoire of applications
Silverman, A. D., Karim, A. S., & Jewett, M. C. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0186-3 · Cited by 593
The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology
Garamella, J., Marshall, R., Rustad, M., & Noireaux, V. (2016) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00296 · Cited by 419
Self-citationProgrammable on-chip DNA compartments as artificial cells
Karzbrun, E., Tayar, A. M., Noireaux, V., & Bar-Ziv, R. H. (2014) · Science · doi:10.1126/science.1255550 · Cited by 264
Self-citationSun, Z. Z., Yeung, E., Hayes, C. A., Noireaux, V., & Murray, R. M. (2014) · ACS Synthetic Biology · doi:10.1021/sb400131a · Cited by 279
Self-citationAlessa, O., Aiba, Y., Arbaah, M., Hidaka, Y., Watanabe, S., Miyanaga, K., Wannigama, D. L., & Cui, L. (2025) · Molecules · doi:10.3390/molecules30153132 · Cited by 21
Metadata
- Authors
- Shin, J., Jardine, P., & Noireaux, V.
- Venue
- ACS Synthetic Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-07-10
- Volume
- 1
- Issue
- 9
- Pages
- 408-413
- ISSN
- 2161-5063 (ISSN-L)
- OpenAlex
- W2156021274
- Cited by
- 162
- References
- 2
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction. https://app.cochat.ai/papers/10.1021/sb300049p
- The paper
- Shin, J., Jardine, P., & Noireaux, V. (2012). Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction. ACS Synthetic Biology. https://doi.org/10.1021/sb300049p
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
