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A Golden Gate Modular Cloning Toolbox for Plants
Abstract
Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.
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Cited by
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Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015) · ACS Synthetic Biology · doi:10.1021/sb500366v · Cited by 916
Lawrenson, T., Shorinola, O., Stacey, N., Li, C., Østergaard, L., Patron, N., Uauy, C., & Harwood, W. (2015) · Genome Biology · doi:10.1186/s13059-015-0826-7 · Cited by 551
Self-citationA Coevolved EDS1-SAG101-NRG1 Module Mediates Cell Death Signaling by TIR-Domain Immune Receptors
Lapin, D., Kovacova, V., Sun, X., Dongus, J. A., Bhandari, D., von Born, P., Bautor, J., Guarneri, N., Rzemieniewski, J., Stuttmann, J., et al. (2019) · The Plant Cell · doi:10.1105/tpc.19.00118 · Cited by 303
Crozet, P., Navarro, F. J., Willmund, F., Mehrshahi, P., Bakowski, K., Lauersen, K. J., Pérez-Pérez, M.-E., Auroy, P., Gorchs Rovira, A., Sauret-Gueto, S., et al. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00251 · Cited by 297
Recent advancements in the genetic engineering of microalgae
Sproles, A. E., Fields, F. J., Smalley, T. N., Le, C. H., Badary, A., & Mayfield, S. P. (2021) · Algal Research · doi:10.1016/j.algal.2020.102158 · Cited by 228
Metadata
- Authors
- Engler, C., Youles, M., Gruetzner, R., Ehnert, T.-M., Werner, S., Jones, J. D. G., Patron, N. J., & Marillonnet, S.
- Venue
- ACS Synthetic Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-02-20
- Volume
- 3
- Issue
- 11
- Pages
- 839-843
- ISSN
- 2161-5063 (ISSN-L)
- OpenAlex
- W2073936714
- Cited by
- 928
- References
- 0
- FWCI
- 7.67
- Type
- journal article
- Language
- en
- Open access
- closed
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- Engler, C., Youles, M., Gruetzner, R., Ehnert, T.-M., Werner, S., Jones, J. D. G., Patron, N. J., & Marillonnet, S. (2014). A Golden Gate Modular Cloning Toolbox for Plants. ACS Synthetic Biology. https://doi.org/10.1021/sb4001504
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