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Binding of Perfluorooctanoic Acid to Rat and Human Plasma Proteins
Abstract
Perfluorooctanoic acid (PFOA) is a commercially important organic fluorochemical and is considered to have a long half-life in human blood. In this paper, PFOA binding to rat and human plasma proteins was investigated. On the basis of results from size-exclusion chromatography and ligand blotting, most PFOA was in protein-bound form in male and female rat plasma, and the primary PFOA binding protein in plasma was serum albumin. PFOA binding to rat serum albumin (RSA) in the gas phase was observed by electrospray ionization MS. (19)F NMR experiments revealed that binding to RSA caused peak broadening and chemical shift changes of PFOA resonances, and on the basis of this observation, the dissociation constant was determined to be approximately 0.3 mM. The dissociation constants for PFOA binding to RSA and human serum albumin (HSA) and the numbers of PFOA binding sites on RSA and HSA were also determined by a separation method using microdesalting columns. No significant difference was found between PFOA binding to RSA and PFOA binding to HSA. The dissociation constants for binding of PFOA to RSA or HSA and the numbers of PFOA binding sites were in the range of 0.3-0.4 mM and 6-9, respectively. On the basis of these binding parameters and the estimated plasma concentration of serum albumin, greater than 90% of PFOA would be bound to serum albumin in both rat and human blood.
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Research works printed but not linked: 53
- Banks R. E., B. E., and Tatlow, J. C. (1994) In Organofluorine Chemistry: Principles and Commercial Applications
- Taves D. R., 1968, Evidence that there are two forms of fluoride in human serum. Nature 217, 1050−1051
- Guy W. S., 1976, ACS Symposium Series No. 28 (Filler, R., Ed.), 134
- Ubel F. A., 1980, Am. Ind. Hyg. Assoc. J., 41
- Hanhijarvi H., 1982, Proc. Soc. Exp. Biol. Med., 171, 55, 10.3181/00379727-171-41476 · DOI
- Hanhijarvi H., 1987, Pharmacol. Toxicol., 61, 68, 10.1111/j.1600-0773.1987.tb01775.x · DOI
- Ylinen M., 1990, Bull. Environ. Contam. Toxicol., 44, 53, 10.1007/BF01702360 · DOI
- Kuslikis B. I., 1991, J. Biochem. Toxicol., 6, 92
- Vanden Heuvel J. P., 1992, J. Biochem. Toxicol., 7, 36, 10.1002/jbt.2570070107 · DOI
- Ikeda T., 1985, J. Biochem., 98, 10.1093/oxfordjournals.jbchem.a135302 · DOI
- Pastoor T. P., 1987, Exp. Mol. Pathol., 47, 109, 10.1016/0014-4800(87)90011-6 · DOI
- Sohlenius A. K., 1992, Biochem. J., 285, 10.1042/bj2850779 · DOI
- Kawashima Y., 1989, Biochem. J., 261, 10.1042/bj2610595 · DOI
- Kudo N., 2000, Induction by perfluorinated fatty acids with different carbon chain length of peroxisomal β-oxidation in the liver of rats. Chem.-Biol. Interact. 124, 119−132
- Gilliland F. D., 1996, Am. J. Ind. Med., 29, 10.1002/(SICI)1097-0274(199605)29:5<560::AID-AJIM17>3.0.CO;2-Z · DOI
- Ophaug R. H., 1980, Proc. Soc. Exp. Biol. Med., 163, 23, 10.3181/00379727-163-40715 · DOI
- Kronis K. A., 1982, Specificity of isolectins of wheat germ agglutinin for sialyloligosaccharides: a 360-MHz proton nuclear magnetic resonance binding study. Biochemistry 21, 3050−3057
- Sauter, N. K., Bednarski, M. D., Wurzburg, B. A., Hanson, J. E., Whitesides, G. M., Skehel, J. J., and Wiley, D. C. (1989) Hemagglutinins from two influenza virus variants bind to sialic acid derivatives with millimolar dissociation constants: a 500-MHz proton nuclear magnetic resonance study.Biochemistry28, 8388−8396. · DOI · Paper page
- Hammes G. G., 2000, Thermodynamics and Kinetics for the Biological Sciences, 125
- Pryzdial E. L., 1996, J. Biol. Chem., 271
- Pryzdial E. L., 1999, J. Biol. Chem., 274, 10.1074/jbc.274.13.8500 · DOI
- Bocharov A. V., 2001, Characterization of a 95 kDa 781 high affinity human high-density lipoprotein-binding protein. Biochemistry 40, 4407−4416
- Anderson O. M., 2001, Biochem. J., 357
- Loo J. A., 1997, Mass Spectrom. Rev., 16, 23
- Pramanik B. N., 1998, J. Mass Spectrom., 33, 10.1002/(SICI)1096-9888(1998100)33:10<911::AID-JMS737>3.0.CO;2-5 · DOI
- Veenstra T. D., 1999, Biochem. Biophys. Res. Commun., 257, 5, 10.1006/bbrc.1998.0103 · DOI
- Van der Kerk, 1999, J. Mass Spectrom., 34
- Ayed, A., Krutchinsky, A. N., Ens, W., Standing, K. G., and Duckworth, H. W. (1998) Quantitative evaluation of protein−protein and ligand-protein equilibria of a large allosteric enzyme by electrospray ionization time-of-flight mass spectrometry.Rapid Commun. Mass Spectrom.12, 339−344. · DOI
- de Brouwer A. P. M., 2002, Determination of the stability of the noncovalent phospholipid transfer protein−lipid complex by electrospray time-of-flight mass spectrometry. Biochemistry 41, 8013−8018
- Ray S. S., 2001, J. Am. Soc. Mass Spectrom., 12
- Ishigai M., 2000, J. Am. Soc. Mass Spectrom., 11, 10.1016/S1044-0305(00)00127-6 · DOI
- Witkowska H. E., 1996, Intact noncovalent dimer of estrogen receptor ligand-binding domain can be detected by electrospray ionization mass spectrometry. Steroids 61, 433−438
- Goecke C. M., 1992, A comparative toxicological investigation of perfluorocarboxylic acids in rats by fluorine-19 NMR spectroscopy. Chem. Res. Toxicol. 5, 512−519
- Wright J. D., 1996, Measurement and analysis of unbound drug concentrations. Clin. Pharmacokinet. 30, 445−462
- Sorrentino D., 1994, Am. J. Physiol., 266
- Pardridge W. M., 1980, Am. J. Physiol., 239
- Forker E. L., 1983, J. Clin. Invest., 72, 10.1172/JCI111136 · DOI
- Forker E. L., 1982, J. Pharmacol. Exp. Ther., 223
- Weisiger R., 1981, DC 211, 1048−1051.
- Hutter J. F., 1984, J. Mol. Cell. Cardiol., 16
- Nunes R., 1988, J. Hepatol., 7, 10.1016/S0168-8278(88)80001-1 · DOI
- Sorrentino D., 1989, J. Clin. Invest., 84, 10.1172/JCI114301 · DOI
- Pond S. M., 1992, Am. J. Physiol., 262
- Burczynski F. J., 1994, Am. J. Physiol., 267
- Burczynski F. J., 1989, Am. J. Physiol., 257
- Fleischer A. B., 1986, J. Clin. Invest., 77, 10.1172/JCI112397 · DOI
- Besseghir K., 1989, Am. J. Physiol., 256
- Hamilton J. A., 1991, Proc. Natl. Acad. Sci. U.S.A. 88
- Sklar L. A., 1977, Conjugated polyene fatty acids as fluorescent probes: binding to bovine serum albumin. Biochemistry 16, 5100−5108
- Reed R. G., 1986, J. Biol. Chem., 261
- Spector A., 1975, J. Lipid Res., 16, 10.1016/S0022-2275(20)36723-7 · DOI · Paper page
- Kunleda H., 1976, J. Phys. Chem., 80
- Abbreviations ADE, absorption, distribution, and elimination
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The toxicology of perfluorooctanoate
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Metadata
- Authors
- Han, X., Snow, T. A., Kemper, R. A., & Jepson, G. W.
- Venue
- Chemical Research in Toxicology
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-05-16
- Volume
- 16
- Issue
- 6
- Pages
- 775-781
- ISSN
- 0893-228X (ISSN-L)
- 1520-5010
- OpenAlex
- W2043672781
- Cited by
- 408
- References
- 0
- FWCI
- 5.64
- Type
- journal article
- Language
- en
- Open access
- closed
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