The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Reactions of 1-Methyl-2-phenylindole with Malondialdehyde and 4-Hydroxyalkenals. Analytical Applications to a Colorimetric Assay of Lipid Peroxidation
Abstract
Under acidic and mild-temperature conditions, 1-methyl-2-phenylindole was found to react with malondialdehyde (MDA) and 4-hydroxyalkenals to yield a stable chromophore with intense maximal absorbance at 586 nm. The use of methanesulfonic acid results in optimal yields of chromophore produced from MDA as well as from 4-hydroxynonenal. By contrast, the use of hydrochloric acid results in an optimal yield of chromophore produced from MDA and a negligible reaction of 4-hydroxynonenal. Taking advantage of such chromogenic reactions, we developed a new colorimetric assay of lipid peroxidation. Using a methanesulfonic acid-based medium, MDA and 4-hydroxyalkenals can be measured at the 586 nm wavelength. However, the presence of endogenous inhibitors of the reaction with 4-hydroxyalkenals is common, and this means that the latter may be underestimated in some biological samples. The assay performed in a hydrochloric acid-based medium enables the specific measurement of MDA in the presence of 4-hydroxyalkenals. Upon hydrolysis of Schiff bases in hydrochloric acid (pH 1.5), either assay can be used to specifically measure the amount of total MDA in biological samples because 4-hydroxyalkenals undergo an irreversible cyclization reaction under the hydrochloric acid-based conditions of hydrolysis. The two assays were applied to the determination of the amount of MDA alone and of MDA and 4-hydroxyalkenals in an in vitro model of lipid peroxidation. This methodology was also used to clarify complex patterns of tissue-specific MDA production in vivo, following hydrolysis of Schiff bases, in rodents treated with doxorubicin.
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Cites
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Cited by
Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish
Choi, J. E., Kim, S., Ahn, J. H., Youn, P., Kang, J. S., Park, K., Yi, J., & Ryu, D.-Y. (2010) · Aquatic Toxicology · doi:10.1016/j.aquatox.2009.12.012 · Cited by 494
Hu, C., Nydes, M., Shanley, K. L., Morales Pantoja, I. E., Howard, T. A., & Bizzozero, O. A. (2019) · Journal of Neurochemistry · doi:10.1111/jnc.14604 · Cited by 200
Targeting ferroptosis protects against experimental (multi)organ dysfunction and death
Van Coillie, S., Van San, E., Goetschalckx, I., Wiernicki, B., Mukhopadhyay, B., Tonnus, W., Choi, S. M., Roelandt, R., Dumitrascu, C., Lamberts, L., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-28718-6 · Cited by 186
Lipoxidation in cardiovascular diseases
Gianazza, E., Brioschi, M., Fernandez, A. M., & Banfi, C. (2019) · Redox Biology · doi:10.1016/j.redox.2019.101119 · Cited by 152
Van San, E., Debruyne, A. C., Veeckmans, G., Tyurina, Y. Y., Tyurin, V. A., Zheng, H., Choi, S. M., Augustyns, K., van Loo, G., Michalke, B., et al. (2023) · Cell Death & Differentiation · doi:10.1038/s41418-023-01195-0 · Cited by 103
“Colorimetric MDA assay As described previously [67], an estimation of malondialdehyde (MDA) levels was made via colorimetric measurement of 1-methyl-2phenylindole reactive species.”
Method
Metadata
- Authors
- Gérard-Monnier, D., Erdelmeier, I., Régnard, K., Moze-Henry, N., Yadan, J.-C., & Chaudière, J.
- Venue
- Chemical Research in Toxicology
- Publisher
- American Chemical Society (ACS)
- Published
- 1998-09-30
- Volume
- 11
- Issue
- 10
- Pages
- 1176-1183
- ISSN
- 0893-228X (ISSN-L)
- 1520-5010
- OpenAlex
- W2064473116
- Cited by
- 494
- References
- 36
- FWCI
- 0.93
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Reactions of 1-Methyl-2-phenylindole with Malondialdehyde and 4-Hydroxyalkenals. Analytical Applications to a Colorimetric Assay of Lipid Peroxidation. https://app.cochat.ai/papers/10.1021/tx9701790
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- Gérard-Monnier, D., Erdelmeier, I., Régnard, K., Moze-Henry, N., Yadan, J.-C., & Chaudière, J. (1998). Reactions of 1-Methyl-2-phenylindole with Malondialdehyde and 4-Hydroxyalkenals. Analytical Applications to a Colorimetric Assay of Lipid Peroxidation. Chemical Research in Toxicology. https://doi.org/10.1021/tx9701790
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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