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Cell adhesion and proliferation on biomimetic calcium-phosphate coatings produced by a sodium silicate gel methodology
Open accessAbstract
The present study describes a methodology to produce bioactive coatings on the surface of starch based biodegradable polymers or other polymeric biomaterials. As an alternative to the more typical bioactive glass percursors, a sodium silicate gel is being employed as a nucleating agent, for inducing the formation of a calcium-phosphate (Ca-P) layer. The method has the advantage of being able to coat efficiently both compact materials and porous 3D architectures aimed at being used on tissue replacement applications and as bone tissue engineering scaffolds. This treatment is also very effective in reducing the incubation periods, being possible to observe the formation of an apatite-like layer, only after 6 h of immersion in a simulated body fluid (SBF). The influence of the SBF concentration on the formation of the apatite coating was also studied. The apatite coatings formed under different conditions were analyzed and compared in terms of morphology, chemical composition and structure. After the first days of SBF immersion, the apatite-like films exhibit the typical cauliflower like morphology. With increasing immersion times, these films exhibited a partially amorphous nature and the Ca/P ratios became very closer to the value attributed to hydroxyapatite (1.67). The obtained results are very promising for pre-calcifying bone tissue engineering scaffolds. Therefore, in order to study cell behavior and response to these apatite coatings, adhesion, morphology, and proliferation of a human osteoblast cell line (SaOS-2) was also analyzed after being cultured in the coatings formed after 15 days of immersion in SBF. Results indicate a good correlation between crystallinity of the apatite like coatings formed in these conditions and respective cell spreading and morphology. In general, higher cell proliferation was observed for higher crystalline Ca-P coatings.
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bCaP has high resemblance to natural bone mineral and ability to stimulate regenerative processes such as enhanced cell adhesion and proliferation, and improved fixation to host tissue.
“bCaP was chosen due to its high resemblance to natural bone mineral and ability to stimulate various regenerative processes such as enhanced cell adhesion and proliferation, and improved fixation to the host tissue.[ 222 ]”
From Unraveling and Harnessing the Immune Response at the Cell–Biomaterial Interface for Tissue Engineering Purposes (2024) · cites this paper as [222]
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Self-citation
Metadata
- Authors
- Oliveira, A. L., Alves, C. M., & Reis, R. L.
- Affiliations
- University of Minho (PT)
- 3B's Research Group (PT)
- Corresponding author
- Oliveira, A. L.
- Venue
- Journal of Materials Science: Materials in Medicine
- Published
- 2002-12
- Volume
- 13
- Issue
- 12
- Pages
- 1181-1188
- ISSN
- 0957-4530 (ISSN-L)
- 1573-4838
- OpenAlex
- W1514992005
- Cited by
- 33
- References
- 26
- Citations by year
- 2024: 3 · 2020: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2013: 1 · 2012: 2
- FWCI
- 2.39
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Journal of Materials Science Materials in Medicine (publishedVersion)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Engineering › Mechanical Engineering › Surface Treatment and Coatings
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Keywords
- calcium phosphate coatings, sodium silicate gel, apatite formation, simulated body fluid, bioactive coatings, bone scaffolds, osteoblast adhesion, cell proliferation, Saos-2 cells, coating crystallinity, biomimetic mineralization
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