Immortalized human hepatocytes as a tool for the study of hepatocytic (de-)differentiation
Abstract
Primary human hepatocytes were immortalized by stable transfection with a recombinant plasmid containing the early region of simian virus (SV) 40. The cells were cultured in serum-free, hormonally defined medium during the immortalization procedure. Foci of dividing cells were seen after 3 months. Albumin- and fibrinogen-secreting cells were selected and cloned by limiting dilution to obtain homologous cell populations. The established IHH (immortalized human hepatocyte) cell lines were evaluated for their usefulness in studying the regulation of cell growth and of certain differentiated hepatocyte functions. IHH cells retain several differentiated features of normal hepatocytes. They display albumin secretion at a level comparable to cultured primary human hepatocytes (30 micrograms albumin/ml per day). A portion of the IHH cells are polarized, forming bile canaliculi-like vacuoles where exogeneous organic anions accumulate. The multidrug resistance (MDR) P-glycoprotein, known to be localized at the canalicular membrane, is also present in these vacuoles. The polarized features allowed the use of IHH cells for the study of localization of the newly characterized multidrug resistance protein MRP1. The homologues of MRP were found in hepatocytes, MRP1 and MRP2 (cMOAT), both functioning in ATP-dependent excretion of anionic conjugates. In differentiated hepatocytes, MRP1 expression is extremely low. In contrast, MRP1 is highly expressed in proliferating IHH cells, where it is l…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Similar data were obtained using a non-cancerous human immortalized hepatic cell-line (IHH) used as another model of partially dedifferentiated hepatocytes.
“Similar data were obtained using a non‐cancerous human immortalized hepatic cell‐line (IHH) used as another model of partially dedifferentiated hepatocytes (Schippers et al, 1997; Fig EV4A–C).”
From An extended transcription factor regulatory network controls hepatocyte identity (2023) · cites this paper as Schippers et al, 1997
Cites
Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line
Aden, D. P., Fogel, A., Plotkin, S. A., Damjanov, I., & Knowles, B. B. (1979) · Nature · doi:10.1038/282615a0 · Cited by 1,317 · see citing works
Separation of plasma lipoproteins by density-gradient ultracentrifugation
Redgrave, T. G., Roberts, D., & West, C. E. (1975) · Analytical Biochemistry · doi:10.1016/0003-2697(75)90488-1 · Cited by 1,101 · see citing works
Congenital Jaundice in Rats with a Mutation in a Multidrug Resistance-Associated Protein Gene
Paulusma, C. C., Bosma, P. J., Zaman, G. J. R., Bakker, C., Otter, M. Y., Scheffer, G. L., Scheper, R. J., Borst, P., & Elferink, R. P. J. O. (1996) · Science · doi:10.1126/science.271.5252.1126 · Cited by 817 · see citing works
Hepatocyte function and extracellular matrix geometry: long‐term culture in a sandwich configuration
Dunn, J., Yarmush, M. L., Koebe, H. G., & Tompkins, R. G. (1989) · The FASEB Journal · doi:10.1096/fasebj.3.2.2914628 · Cited by 730 · see citing works
Guguen‐Guillouzo, C., Clément, B., Baffet, G., Beaumont, C., Morel-Chany, E., Glaise, D., & Guillouzo, A. (1983) · Experimental Cell Research · doi:10.1016/0014-4827(83)90107-6 · Cited by 517 · see citing works
Hagenbuch, B., & Meier, P. J. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117091 · Cited by 460 · see citing works
Dixon, J., & Ginsberg, H. N. (1993) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)40744-8 · Cited by 368 · see citing works
Enat, R., Jefferson, D. M., Ruiz‐Opazo, N., Gatmaitan, Z., Leinwand, L. A., & Reid, L. M. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.5.1411 · Cited by 362 · see citing works
Hep G2 cells as a resource for metabolic studies: lipoprotein, cholesterol, and bile acids
Javitt, N. B. (1990) · The FASEB Journal · doi:10.1096/fasebj.4.2.2153592 · Cited by 357 · see citing works
Pfeifer, A. M. A., Cole, K. E., Smoot, D. T., Weston, A., Groopman, J. D., Shields, P. G., Vignaud, J.-M., Juillerat, M., Lipsky, M. M., & Trump, B. F. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.11.5123 · Cited by 240 · see citing works
Roelofsen, H., Vos, T. A., Schippers, I. J., Kuipers, F., Koning, H., Moshage, H. J., Jansen, P. L. M., & Müller, M. M. (1997) · Gastroenterology · doi:10.1053/gast.1997.v112.pm9024305 · Cited by 141 · see citing works
Self-citationEllsworth, J. L., Erickson, S. K., & Cooper, A. D. (1988) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38780-0 · Cited by 140 · see citing works
Liang, D., Hagenbuch, B., Stieger, B., & Meier, P. J. (1993) · Hepatology · doi:10.1002/hep.1840180523 · Cited by 106 · see citing works
Woodworth, C. D., Secott, T. E., & Isom, H. C. (1986) · PubMed · Cited by 91 · see citing works
Kono, Y., Yang, S., Letarte, M., & Roberts, E. A. (1995) · Experimental Cell Research · doi:10.1006/excr.1995.1399 · Cited by 66 · see citing works
Characterization of human hepatocyte lines derived from normal liver tissue
Roberts, E. A., Letarte, M., Squire, J. A., & Yang, S. (1994) · Hepatology · doi:10.1002/hep.1840190612 · Cited by 64 · see citing works
Donato, M. T., Castell, J. V., & G�mez-Lech�n, M. J. (1991) · Cell Biology and Toxicology · doi:10.1007/bf00121326 · Cited by 61 · see citing works
Lin, Y., Havinga, R., Verkade, H. J., Moshage, H. J., Slooff, M. J. H., Vonk, R. J., & Kuipers, F. (1996) · Hepatology · doi:10.1002/hep.510230204 · Cited by 53 · see citing works
Self-citationCharacteristics and regulation of bile salt synthesis and secretion by human hepatoma HepG2 cells
Cooper, A. D., Craig, W. Y., Taniguchi, T., & Everson, G. T. (1994) · Hepatology · doi:10.1002/hep.1840200623 · Cited by 31 · see citing works
Moshage, H. J., & Yap, S. H. (1992) · Journal of Hepatology · doi:10.1016/0168-8278(92)90078-4 · Cited by 26 · see citing works
Self-citationDinsart, C., Cornelis, J. J., Klein, B., van der Eb, A. J., & Rommelaere, J. (1984) · Molecular and Cellular Biology · doi:10.1128/mcb.4.2.324 · Cited by 21 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Ectopic β-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis
Martinez, L. O., Jacquet, S., Estève, J., Rolland, C., Cabezón, E., Champagne, É., Pineau, T., Georgeaud, V., Walker, J. E., Tercé, F., et al. (2003) · Nature · doi:10.1038/nature01250 · Cited by 459 · see citing works
Generation of Hepatocyte-Like Cells from Human Embryonic Stem Cells
Rambhatla, L., Chiu, C.-P., Kundu, P., Peng, Y., & Carpenter, M. K. (2003) · Cell Transplantation · doi:10.3727/000000003783985179 · Cited by 437 · see citing works
Hepatocyte cell lines: their use, scope and limitations in drug metabolism studies
Castell, J. V., Jover, R., Martinez‐Jimenez, C. P., & G�mez-Lech�n, M. J. (2006) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425255.2.2.183 · Cited by 209 · see citing works
Barbier, O., Pineda‐Torra, I., Sirvent, A., Claudel, T., Blanquart, C., Durán-Sandoval, D., Kuipers, F., Kosykh, V., Fruchart, J., & Staels, B. (2003) · Gastroenterology · doi:10.1016/s0016-5085(03)00388-3 · Cited by 203 · see citing works
Self-citationKuipers, F., Jong, M. C., Lin, Y., Eck, M. V., Havinga, R., Bloks, V. W., Verkade, H. J., Hofker, M. H., Moshage, H. J., van Berkel, T. J. C., et al. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119841 · Cited by 167 · see citing works
Self-citationCaron, S., Verrijken, A., Mertens, I. L., Samanez, C. H., Mautino, G., Haas, J. T., Duran-Sandoval, D., Prawitt, J., Francque, S., Vallez, E., et al. (2010) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.110.220723 · Cited by 162 · see citing works
Self-citationLong-term culture and expansion of primary human hepatocytes
Levy, G., Bomze, D., Heinz, S., Ramachandran, S. D., Noerenberg, A., Cohen, M., Shibolet, O., Sklan, E., Braspenning, J., & Nahmias, Y. (2015) · Nature Biotechnology · doi:10.1038/nbt.3377 · Cited by 155 · see citing works
Caron, S., Samanez, C. H., Dehondt, H., Ploton, M., Briand, O., Lien, F., Dorchies, E., Dumont, J., Postic, C., Cariou, B., et al. (2013) · Molecular and Cellular Biology · doi:10.1128/mcb.01004-12 · Cited by 151 · see citing works
Jansen, E. J. R., Timal, S., Ryan, M., Ashikov, A., van Scherpenzeel, M., Graham, L. A., Mandel, H., Hoischen, A., Iancu, T. C., Raymond, K., et al. (2016) · Nature Communications · doi:10.1038/ncomms11600 · Cited by 149 · see citing works
Cariou, B., Ouguerram, K., Zaïr, Y., Guérois, R., Langhi, C., Kourimate, S., Benoit, I., May, C. L., Gayet, C., Belabbas, K., et al. (2009) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.109.194191 · Cited by 139 · see citing works
Nagarajan, S. R., Paul-Heng, M., Krycer, J. R., Fazakerley, D. J., Sharland, A. F., & Hoy, A. J. (2019) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00365.2018 · Cited by 132 · see citing works
Liver tissue engineering and cell sources: issues and challenges
Palakkan, A. A., Hay, D. C., PR, A. K., TV, K., & Ross, J. A. (2013) · Liver International · doi:10.1111/liv.12134 · Cited by 130 · see citing works
Hepatocyte transplantation: clinical and experimental application
Ohashi, K., Park, F., & Kay, M. A. (2001) · Journal of Molecular Medicine · doi:10.1007/s001090100260 · Cited by 128 · see citing works
Human hepatocyte carcinogenesis
Shiraha, H., Yamamoto, K., & Namba, M. (2013) · International Journal of Oncology · doi:10.3892/ijo.2013.1829 · Cited by 123 · see citing works
Strategies for immortalization of primary hepatocytes
Ramboer, E., Craene, B. D., Kock, J. D., Vanhaecke, T., Berx, G., Rogiers, V., & Vinken, M. (2014) · Journal of Hepatology · doi:10.1016/j.jhep.2014.05.046 · Cited by 121 · see citing works
The farnesoid X receptor induces very low density lipoprotein receptor gene expression
Sirvent, A., Claudel, T., Martin, G., Brozek, J., Kosykh, V., Darteil, R., Hum, D. W., Fruchart, J., & Staels, B. (2004) · FEBS Letters · doi:10.1016/j.febslet.2004.04.026 · Cited by 109 · see citing works
Maier, K., Hofmann, U., Reuß, M., & Mauch, K. (2007) · Biotechnology and Bioengineering · doi:10.1002/bit.21746 · Cited by 96 · see citing works
Dual Mechanisms for the Fibrate-mediated Repression of Proprotein Convertase Subtilisin/Kexin Type 9
Kourimate, S., May, C. L., Langhi, C., Jarnoux, A. L., Ouguerram, K., Zaïr, Y., Nguyen, P., Krempf, M., Cariou, B., & Costet, P. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m705831200 · Cited by 95 · see citing works
van der Wijst, M. G. P., van Tilburg, A. Y., Ruiters, M. H. J., & Rots, M. G. (2017) · Scientific Reports · doi:10.1038/s41598-017-00263-z · Cited by 92 · see citing works
Self-citationBrown, J. J., Parashar, B., Moshage, H. J., Tanaka, K. E., Engelhardt, D., Rabbani, E., Roy‐Chowdhury, N., & Roy‐Chowdhury, J. (2000) · Hepatology · doi:10.1002/hep.510310126 · Cited by 89 · see citing works
Self-citationPerttilä, J., Samanez, C. H., Caron, S., Tanhuanpää, K., Staels, B., Yki‐Järvinen, H., & Olkkonen, V. M. (2012) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00125.2011 · Cited by 87 · see citing works
Atanasovska, B., Rensen, S. S., van der Sijde, M. R., Marsman, G., Kumar, V., Jonkers, I. H., Withoff, S., Shiri‐Sverdlov, R., Greve, J. W. M., Faber, K. N., et al. (2017) · Hepatology · doi:10.1002/hep.29034 · Cited by 86 · see citing works
Self-citationDoes endoplasmic reticulum stress participate in APD-induced hepatic metabolic dysregulation?
Lauressergues, E., Bert, E., Duriez, P., Hum, D. W., Majd, Z., Staels, B., & Cussac, D. (2011) · Neuropharmacology · doi:10.1016/j.neuropharm.2011.08.048 · Cited by 80 · see citing works
MEK–ERK Inhibition Corrects the Defect in VLDL Assembly in HepG2 Cells
Tsai, J., Qiu, W., Avramoglu, R. K., & Adeli, K. (2006) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000249861.80471.96 · Cited by 78 · see citing works
Samanez, C. H., Caron, S., Briand, O., Dehondt, H., Duplan, I., Kuipers, F., Hennuyer, N., Barthelemy-Clavey, V., & Staels, B. (2012) · Archives of Physiology and Biochemistry · doi:10.3109/13813455.2012.683442 · Cited by 66 · see citing works
Self-citationCadmium induces mitochondria-dependent apoptosis of normal human hepatocytes
Lasfer, M., Vadrot, N., Aoudjehane, L., Conti, F., Bringuier, A. F., Feldmann, G., & Reyl-Desmars, F. (2007) · Cell Biology and Toxicology · doi:10.1007/s10565-007-9015-0 · Cited by 63 · see citing works
Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes
Ramboer, E., Vanhaecke, T., Rogiers, V., & Vinken, M. (2014) · Methods in molecular biology · doi:10.1007/978-1-4939-2074-7_4 · Cited by 63 · see citing works
Rada, M., Kapelanski‐Lamoureux, A., Petrillo, S. K., Tabariès, S., Siegel, P. M., Reynolds, A. R., Lazaris, A., & Metrakos, P. P. (2021) · Communications Biology · doi:10.1038/s42003-021-02481-8 · Cited by 62 · see citing works
N‐Acetylcysteine does not Protect HepG2 Cells against Acetaminophen‐Induced Apoptosis
Manov, I., Hirsh, M., & Iancu, T. C. (2004) · Basic & Clinical Pharmacology & Toxicology · doi:10.1111/j.1742-7843.2004.pto940504.x · Cited by 62 · see citing works
Gene and functional up-regulation of the BCRP/ABCG2 transporter in hepatocellular carcinoma
Sukowati, C. H. C., Rosso, N., Pascut, D., Anfuso, B., Torre, G., Francalanci, P., Crocè, L. S., & Tiribelli, C. (2012) · BMC Gastroenterology · doi:10.1186/1471-230x-12-160 · Cited by 61 · see citing works
RNA-binding protein ZFP36L1 maintains posttranscriptional regulation of bile acid metabolism
Tarling, E. J., Clifford, B. L., Cheng, J., Morand, P., Cheng, A., Lester, E., Sallam, T., Turner, M., & Vallim, T. Q. D. A. (2017) · Journal of Clinical Investigation · doi:10.1172/jci94029 · Cited by 59 · see citing works
Generation of proliferating human adult hepatocytes using optimized 3D culture conditions
Rose, S., Ezan, F., Cuvellier, M., Bruyère, A., Legagneux, V., Langouët, S., & Baffet, G. (2021) · Scientific Reports · doi:10.1038/s41598-020-80019-4 · Cited by 58 · see citing works
Recent Developments on Human Cell Lines for the Bioartificial Liver
Hoekstra, R., & Chamuleau, R. A. F. M. (2002) · The International Journal of Artificial Organs · doi:10.1177/039139880202500304 · Cited by 58 · see citing works
Liver Support Therapy: An Overview of the AMC-Bioartificial Liver Research
van de Kerkhove, M. P., Poyck, P. P. C., Deurholt, T., Hoekstra, R., Chamuleau, R. A. F. M., & van Gulik, T. M. (2005) · Digestive Surgery · doi:10.1159/000088055 · Cited by 56 · see citing works
Farnesoid X receptor represses hepatic lipase gene expression
Sirvent, A., Verhoeven, A. J. M., Jansen, H., Kosykh, V., Darteil, R., Hum, D. W., Fruchart, J., & Staels, B. (2004) · Journal of Lipid Research · doi:10.1194/jlr.m400221-jlr200 · Cited by 54 · see citing works
L’homme, L., Sermikli, B. P., Haas, J. T., Fleury, S., Quemener, S., Guinot, V., Barreby, E., Esser, N., Caiazzo, R. J., Verkindt, H., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-51078-2 · Cited by 52 · see citing works
Chronic hyperinsulinemia promotes human hepatocyte senescence
Baboota, R. K., Spinelli, R., Erlandsson, M. C., Brandão, B. B., Lino, M., Yang, H., Mardinoğlu, A., Bokarewa, M. I., Boucher, J., Kahn, C. R., et al. (2022) · Molecular Metabolism · doi:10.1016/j.molmet.2022.101558 · Cited by 47 · see citing works
Loregger, A., Cook, E. C. L., Nelson, J. K., Moeton, M., Sharpe, L. J., Engberg, S., Karimova, M., Lambert, G. C., Brown, A. J., & Zelcer, N. (2015) · Molecular and Cellular Biology · doi:10.1128/mcb.00890-15 · Cited by 47 · see citing works
Engineered Livers for Infectious Diseases
Gural, N., Mâncio-Silva, L., He, J., & Bhatia, S. N. (2017) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2017.11.005 · Cited by 48 · see citing works
Büll, C., Heise, T., Beurskens, D. M. H., Riemersma, M., Ashikov, A., Rutjes, F. P. J. T., Kuppevelt, T. H. V., Lefeber, D. J., den Brok, M. H., Adema, G. J., et al. (2015) · ACS Chemical Biology · doi:10.1021/acschembio.5b00501 · Cited by 46 · see citing works
DNA damage stress induces the expression of Ribosomal Protein S27a gene in a p53-dependent manner
Nosrati, N., Kapoor, N. R., & Kumar, V. (2015) · Gene · doi:10.1016/j.gene.2015.01.014 · Cited by 42 · see citing works
Roelofsen, H., Hooiveld, G. J. E. J., Koning, H., Havinga, R., Jansen, P. L. M., & Müller, M. M. (1999) · Journal of Cell Science · doi:10.1242/jcs.112.9.1395 · Cited by 40 · see citing works
Self-citationRole of PTEN in Oxidative Stress and DNA Damage in the Liver of Whole-Body Pten Haplodeficient Mice
Bankoglu, E. E., Tschopp, O., Schmitt, J., Burkard, P., Jahn, D., Geier, A., & Stopper, H. (2016) · PLoS ONE · doi:10.1371/journal.pone.0166956 · Cited by 39 · see citing works
Nosrati, N., Kapoor, N. R., & Kumar, V. (2014) · FEBS Journal · doi:10.1111/febs.12786 · Cited by 39 · see citing works
Green, C., Pramfalk, C., Morten, K., & Hodson, L. (2014) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00192.2014 · Cited by 38 · see citing works
Shukla, S. K., & Kumar, V. (2012) · FEBS Journal · doi:10.1111/j.1742-4658.2012.08745.x · Cited by 37 · see citing works
Green, C., Parry, S. A., Gunn, P. J., Ceresa, C. D. L., Rosqvist, F., Piché, M., & Hodson, L. (2018) · Hormone Molecular Biology and Clinical Investigation · doi:10.1515/hmbci-2018-0038 · Cited by 37 · see citing works
SERS of cells: What can we learn from cell lysates?
Genova, E. C., Pelin, M., Decorti, G., Stocco, G., Sergo, V., Ventura, A., & Bonifacio, A. (2017) · Analytica Chimica Acta · doi:10.1016/j.aca.2017.12.002 · Cited by 34 · see citing works
The Role of Tissue Oxygen Tension in Dengue Virus Replication
Frakolaki, E., Kaimou, P., Moraiti, M., Kalliampakou, K. I., Karampetsou, K. V., Dotsika, E., Liakos, P., Vassilacopoulou, D., Mavromara, P., Bartenschlager, R. F. W., et al. (2018) · Cells · doi:10.3390/cells7120241 · Cited by 34 · see citing works
Poland, D. C. W., García-Vallejo, J. J., Niessen, H. W. M., Nijmeyer, R., Calafat, J., Hack, C. E., Hof, B. V. H., & van Dijk, W. (2005) · Journal of Leukocyte Biology · doi:10.1189/jlb.1004566 · Cited by 34 · see citing works
Weider, E., Susan‐Resiga, D., Essalmani, R., Hamelin, J., Asselin, M.-C., Nimesh, S., Ashraf, Y., Wycoff, K. L., Zhang, J., Prat, A., et al. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m116.717736 · Cited by 34 · see citing works
Marmugi, A., Lukowicz, C., Lasserre, F., Montagner, A., Polizzi, A., Ducheix, S., Goron, A., Gamet‐Payrastre, L., Gerbal‐Chaloin, S., Pascussi, J., et al. (2016) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2016.05.006 · Cited by 32 · see citing works
Bruschi, F. V., Tardelli, M., Herac, M., Claudel, T., & Trauner, M. (2020) · Liver International · doi:10.1111/liv.14402 · Cited by 32 · see citing works
Deleye, Y., Cotte, A. K., Hannou, S. A., Hennuyer, N., Bernard, L., Derudas, B., Caron, S., Legry, V., Vallez, E., Dorchies, E., et al. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.ra120.012543 · Cited by 30 · see citing works
Fuchs, C. D., Claudel, T., Mlitz, V., Riva, A., Menz, M., Brusilovskaya, K., Haller, F., Baumgartner, M., Königshofer, P., Unger, L. W., et al. (2023) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2023.08.003 · Cited by 26 · see citing works
Dijk, W., Filippo, M. D., Kooijman, S., van Eenige, R., Rimbert, A., Caillaud, A., Thedrez, A., Arnaud, L., Pronk, A. C. M., Garçon, D., et al. (2022) · Circulation · doi:10.1161/circulationaha.121.057978 · Cited by 25 · see citing works
Sachan, V., Dévéhat, M. L., Roubtsova, A., Essalmani, R., Laurendeau, J.-F., Garçon, D., Susan‐Resiga, D., Duval, S., Mikaeeli, S., Hamelin, J., et al. (2023) · Metabolism · doi:10.1016/j.metabol.2023.155736 · Cited by 23 · see citing works
Saxena, N., & Kumar, V. (2014) · PLoS ONE · doi:10.1371/journal.pone.0111256 · Cited by 23 · see citing works
Nelson, J. K., Cook, E. C. L., Loregger, A., Hoeksema, M. A., Scheij, S. S., Kovačević, I., Hordijk, P. L., Ovaa, H., & Zelcer, N. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.698688 · Cited by 23 · see citing works
Liao, B. M., Raddatz, K., Zhong, L., Parker, B. L., Raftery, M. J., & Schmitz‐Peiffer, C. (2014) · PROTEOMICS · doi:10.1002/pmic.201400202 · Cited by 22 · see citing works
Caputo, M., Cansby, E., Kumari, S., Kurhe, Y., Nair, S., Ståhlman, M., Kulkarni, N. M., Borén, J., Marschall, H., Blüher, M., et al. (2021) · Hepatology Communications · doi:10.1002/hep4.1702 · Cited by 21 · see citing works
Rada, M., Tsamchoe, M., Kapelanski‐Lamoureux, A., Hassan, N., Bloom, J., Petrillo, S. K., Kim, D. H., Lazaris, A., & Metrakos, P. P. (2022) · Cancers · doi:10.3390/cancers14051318 · Cited by 20 · see citing works
Alonso‐Peña, M., Espinosa-Escudero, R. A., Herráez, E., Briz, Ó., Cagigal, M. L., Santiago, J. M. G., Ortega‐Alonso, A., Fernández‐Rodríguez, C., Bujanda, L., Sanchez, M. C., et al. (2022) · Hepatology · doi:10.1002/hep.32517 · Cited by 20 · see citing works
Gautherot, J., Claudel, T., Cuperus, F. J. C., Fuchs, C. D., Falguières, T., & Trauner, M. A. (2018) · Journal of Lipid Research · doi:10.1194/jlr.m084145 · Cited by 20 · see citing works
Proprotein convertase 7 (PCSK7) reduces apoA‐V levels
Ashraf, Y., Duval, S., Sachan, V., Essalmani, R., Susan‐Resiga, D., Roubtsova, A., Hamelin, J., Gerhardy, S., Kirchhofer, D., Tagliabracci, V. S., et al. (2020) · FEBS Journal · doi:10.1111/febs.15212 · Cited by 20 · see citing works
Impact of higher-order heme degradation products on hepatic function and hemodynamics
Seidel, R. A., Claudel, T., Schleser, F., Ojha, N. K., Westerhausen, M., Nietzsche, S., Sponholz, C., Cuperus, F. J. C., Coldewey, S. M., Heinemann, S. H., et al. (2017) · Journal of Hepatology · doi:10.1016/j.jhep.2017.03.037 · Cited by 18 · see citing works
Control of cell death/survival balance by the MET dependence receptor
Duplaquet, L., Leroy, C., Vinchent, A., Paget, S., Lefebvre, J., Abeele, F. V., Lancel, S., Giffard, F., Paumelle, R., Bidaux, G., et al. (2020) · eLife · doi:10.7554/elife.50041 · Cited by 18 · see citing works
Sadji-Ouatas, Z., Lasfer, M., Julien, S. G., Feldmann, G., & Reyl-Desmars, F. (2002) · Biochemical Journal · doi:10.1042/bj20020349 · Cited by 18 · see citing works
Kourimate, S., Chétiveaux, M., Jarnoux, A. L., Lalanne, F., & Costet, P. (2009) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2009.02.012 · Cited by 18 · see citing works
Saha, T., Mehrotra, S., Gupta, P., & Kumar, A. (2025) · Biomaterials · doi:10.1016/j.biomaterials.2025.123140 · Cited by 16 · see citing works
Fuchs, C. D., Dixon, E. D., Hendrikx, T., Mlitz, V., Wahlstrom, A. M., Ståhlman, M., Scharnagl, H., Stojaković, T., Binder, C. J., Marschall, H., et al. (2022) · Hepatology Communications · doi:10.1002/hep4.1998 · Cited by 15 · see citing works
Ballatori, N., Henson, J. H., Seward, D. J., Cai, S., Runnegar, M. T. C., Fricker, G., Miller, D. S., & Boyer, J. L. (2006) · Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology · doi:10.1016/j.cbpb.2006.02.005 · Cited by 13 · see citing works
The Novel Antibacterial Compound Walrycin A Induces Human PXR Transcriptional Activity
Berthier, A., Oger, F., Gheeraert, C., Boulahtouf, A., Guével, R. L., Balaguer, P., Staels, B., Salbert, G., & Lefèbvre, P. (2012) · Toxicological Sciences · doi:10.1093/toxsci/kfs073 · Cited by 13 · see citing works
van Leeuwen, E., Wisman, G. B. A., Tervaert, J.-W. C., Debrus-Palmans, L., van Wijk, R. T., Veenstra, R., Molema, G., van der Zee, A. G., van der Meer, J., & Ruiters, M. H. (2001) · PubMed · Cited by 12 · see citing works
Self-citationKalliampakou, K. I., Athanasopoulou, E., Spanou, A., Flemetakis, E., & Tsironi, T. N. (2025) · Scientific Reports · doi:10.1038/s41598-025-98163-0 · Cited by 11 · see citing works
Fernandez-Rojo, M. A., Pearen, M. A., Burgess, A. G., Ikonomopoulou, M. P., Hoang-Le, D., Genz, B., Saggiomo, S. L., Nawaratna, S. S. K., Poli, M., Reissmann, R., et al. (2024) · Science Signaling · doi:10.1126/scisignal.ade4335 · Cited by 11 · see citing works
Functional analysis of natural PCSK 9 mutants in modern and archaic humans
Mikaeeli, S., Susan‐Resiga, D., Girard, E., Ouadda, A. B. D., Day, R. W., Prost, S., & Seidah, N. G. (2019) · FEBS Journal · doi:10.1111/febs.15036 · Cited by 11 · see citing works
Gebhardt, R., Lippert, C., Schneider, A., & Doehmer, J. (1999) · Toxicology in Vitro · doi:10.1016/s0887-2333(99)00020-x · Cited by 10 · see citing works
Priesner, C., Hesse, F., Windgassen, D., KLOCKE, R., Paul, D., & Wagner, R. (2004) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1290/0404031.1 · Cited by 8 · see citing works
He, Y. Z., van Bommel, P. E., Samplonius, D. F., Bremer, E., & Helfrich, W. (2017) · Scientific Reports · doi:10.1038/s41598-017-13530-w · Cited by 8 · see citing works
Rada, M., Kapelanski‐Lamoureux, A., Zlotnik, O., Petrillo, S. K., Lazaris, A., & Metrakos, P. P. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.05.24.493291 · Cited by 8 · see citing works
Guller, M. C., André, J., Legrand, A., Setterblad, N., Mauviel, A., Verrecchia, F., Bleiberg-Daniel, F., & Bernuau, D. (2008) · Molecular Carcinogenesis · doi:10.1002/mc.20492 · Cited by 7 · see citing works
An extended transcription factor regulatory network controls hepatocyte identity
Dubois‐Chevalier, J., Gheeraert, C., Berthier, A., Boulet, C., Dubois, V., Guille, L., Fourcot, M., Marot, G., Gauthier, K., Dubuquoy, L., et al. (2023) · EMBO Reports · doi:10.15252/embr.202357020 · Cited by 6 · see citing works
“Immortalized human hepatocytes (IHH; Schippers et al , 1997) were cultured in William’s E medium (Gibco, 22551022) supplemented with 10% FBS (Dutscher, SV30160.”
Verzijl, C. R. C., van de Peppel, I. P., Eilers, R. E., Bloks, V. W., Wolters, J. C., Koehorst, M., Kloosterhuis, N., Havinga, R., Jalving, M., Struik, D., et al. (2023) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2023.114270 · Cited by 6 · see citing works
Pelin, M., Genova, E. C., Fusco, L., Marisat, M., Hofmann, U., Favretto, D., Lucafò, M., Taddio, A., Martelossi, S., Ventura, A., et al. (2017) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2017.08.009 · Cited by 6 · see citing works
Impact of Liver Inflammation on Bile Acid Side Chain Shortening and Amidation
Alonso‐Peña, M., Espinosa-Escudero, R. A., Hermanns, H. M., Briz, Ó., Herranz, J. M., García‐Ruiz, C., Fernández-Checa, J. C., Juampérez, J., Avila, M. A., Argemí, J., et al. (2022) · Cells · doi:10.3390/cells11243983 · Cited by 6 · see citing works
Cancer cells induce hepatocytes apoptosis in co-opted colorectal cancer liver metastatic lesionsPreprint
Rada, M., Tsamchoe, M., Kapelanski‐Lamoureux, A., Bloom, J., Petrillo, S. K., Kim, D. H., Lazaris, A., & Metrakos, P. P. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.02.11.429243 · Cited by 6 · see citing works
Applications of Fish Cell Cultures
Aarattuthodiyil, S., & Dharan, V. (2021) · Springer eBooks · doi:10.1007/978-981-16-5195-3_7 · Cited by 5 · see citing works
Requirements for designing organ-on-a-chip platforms to model the pathogenesis of liver disease
Ishida, S. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-817202-5.00005-x · Cited by 5 · see citing works
Zhang, X., Dumčius, P., Mikhaylov, R., Qi, J., Stringer, M., Sun, C., Nguyen, V. D., Zhou, Y., Sun, X., Liang, D., et al. (2024) · Advanced Science · doi:10.1002/advs.202403574 · Cited by 4 · see citing works
A new method to immortalize primary cultured rat hepatocytes
Watanabe, N., Odagiri, H., Totsuka, E., & Sasaki, M. (2004) · Transplantation Proceedings · doi:10.1016/j.transproceed.2004.08.030 · Cited by 4 · see citing works
Development and characterization of a new human hepatic cell line
Ramboer, E., Craene, B. D., Kock, J. D., Berx, G., Rogiers, V., Vanhaecke, T., & Vinken, M. (2015) · EXCLI journal · doi:10.17179/excli2015-424 · Cited by 4 · see citing works
Peroxisomal abnormalities in the immortalized human hepatocyte (IHH) cell line
Klouwer, F. C. C., Koster, J., Ferdinandusse, S., & Waterham, H. R. (2016) · Histochemistry and Cell Biology · doi:10.1007/s00418-016-1532-6 · Cited by 4 · see citing works
Kim, B., Han, Y. S., Choe, B., Cho, H., Nam, G.-D., Lee, J. W., Kim, Y., Park, J. K., Dong, S. H., Kim, H. J., et al. (2005) · Cell Transplantation · doi:10.3727/000000005783982864 · Cited by 3 · see citing works
Fuchs, C. D., Dixon, E. D., Königshofer, P., Claudel, T., Mlitz, V., Scharnagl, H., Stojaković, T., Reiberger, T., & Trauner, M. A. (2025) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2025.101630 · Cited by 2 · see citing works
Liver‐specific lncRNAs associated with liver cancers
Burenina, O. Y., Makarchenko, R. K., Rubtsova, M. P., & Донцова, О. А. (2025) · FEBS Open Bio · doi:10.1002/2211-5463.70079 · Cited by 2 · see citing works
Arellanes‐Robledo, J., Hernández, C., Camacho, J., & Pérez‐Carreón, J. I. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-804274-8.00042-4 · Cited by 2 · see citing works
Котлярова, А. А., & Kotlyarov, S. N. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27104453 · Cited by 1 · see citing works
De novo apoA-I production : regulation and nutritional effects
Dullens, S. P. J. (2008) · doi:10.26481/dis.20081112sd
Original article: DEVELOPMENT AND CHARACTERIZATION OF A NEW HUMAN HEPATIC CELL LINE
Ramboer, E., Craene, B. D., Kock, J. D., Berx, G., Rogiers, V., Vanhaecke, T., & Vinken, M. (2015)
- Authors
- Schippers, I. J., Moshage, H., Roelofsen, H., Müller, M., Heymans, H. S. A., Ruiters, M., & Kuipers, F.
- Affiliations
- University Medical Center Groningen (NL)
- Venue
- Cell Biology and Toxicology
- Published
- 1997-07
- Volume
- 13
- Issue
- 4-5
- Pages
- 375-386
- ISSN
- 0742-2091 (ISSN-L)
- 1573-6822
- OpenAlex
- W1503996899
- Cited by
- 110
- References
- 21
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 4 · 2023: 6 · 2022: 7 · 2021: 5 · 2020: 5 · 2019: 4 · 2018: 4 · 2017: 9 · 2016: 6 · 2015: 7 · 2014: 6 · 2013: 3 · 2012: 5
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Cell Biology and Toxicology (publishedVersion)
- PubMed (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Hepatology › Hepatocellular Carcinoma Treatment and Prognosis
- Keywords
- hepatocyte differentiation, hepatocyte dedifferentiation, SV40 early region, albumin secretion, fibrinogen secretion, P-glycoprotein, MRP2, organic anion transport, taurocholate uptake, triglyceride-rich lipoproteins, apolipoprotein B, apolipoprotein A-I, hepatocyte proliferation, hepatocyte culture
- MeSH terms
- MutS Homolog 3 Protein, Bile Acids and Salts, Blood Proteins, Cell Differentiation, Cell Line, Cell Survival, DNA-Binding Proteins, Enzyme-Linked Immunosorbent Assay, Humans, Liver, Male, Middle Aged, Plasmids, Simian virus 40, Transfection, Triglycerides, Cell Polarity, ATP Binding Cassette Transporter, Subfamily B, Member 1, ATP-Binding Cassette, Sub-Family C Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
