The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Arsenic induces oxidative DNA damage in mammalian cells
Abstract
Although arsenic is a well-established human carcinogen, the underlying carcinogenic mechanism(s) is not known. Using the human-hamster hybrid (A(L)) cell mutagenic assay that is sensitive in detecting mutagens that induce predominately multilocus deletions, we showed previously that arsenite is indeed a potent gene and chromosomal mutagen and that oxyradicals may be involved in the mutagenic process. In the present study, the effects of free radical scavenging enzymes on the cytotoxic and mutagenic potential of arsenic were examined using the AL cells. Concurrent treatment of cells with either superoxide dismutase or catalase reduced both the cytotoxicity and mutagenicity of arsenite by an average of 2-3 fold, respectively. Using immunoperoxidase staining with a monoclonal antibody specific for 8-hydroxy-2'-deoxyguanosine (8-OHdG), we demonstrated that arsenic induced oxidative DNA damage in A(L) cells. This induction was significantly reduced in the presence of the antioxidant enzymes. Furthermore, reducing the intracellular levels of non-protein sulfhydryls (mainly glutathione) using buthionine S-R-Sulfoximine increased the total mutant yield by more than 3-fold as well as the proportion of mutants with multilocus deletions. Taken together, our data provide clear evidence that reactive oxygen species play an important causal role in the genotoxicity of arsenic in mammalian cells.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Chem-otherapeutic agents can generate oxidative DNA damage. For example, artesunate can induce 1,N6-ethenoadenine, SSBs and replication-associated DSBs [23–25].”
From Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress (2014) · cites this paper as [24]
Citations
Cites
Meister, A., & Anderson, M. E. (1983) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.52.070183.003431 · Cited by 5,694
DNA damage and oxygen radical toxicity
Imlay, J. A., & Linn, S. (1988) · Science · doi:10.1126/science.3287616 · Cited by 1,974
8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G-T and A-C substitutions.
Cheng, K. C., Cahill, D. S., Kasai, H., Nishimura, S., & Loeb, L. A. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)48474-8 · Cited by 1,844
Groundwater Arsenic Contamination in Bangladesh and West Bengal, India
Chowdhury, U. K., Biswas, B. K., Chowdhury, T. R., Samanta, G., Mandal, B. K., BASU, G. C., Chanda, C. R., Lodh, D., Saha, K. C., Mukherjee, S. K., et al. (2000) · Environmental Health Perspectives · doi:10.1289/ehp.00108393 · Cited by 900
Induction of oxyradicals by arsenic: Implication for mechanism of genotoxicity
Liu, S. X., Athar, M., Lippai, I., Waldren, C. A., & Hei, T. K. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.4.1643 · Cited by 539
Self-citationReactions of arsenic(III) and arsenic(V) species with glutathione
Scott, N., Hatlelid, K. M., Mackenzie, N. E., & Carter, D. E. (1993) · Chemical Research in Toxicology · doi:10.1021/tx00031a016 · Cited by 458
Mutagenicity of arsenic in mammalian cells: Role of reactive oxygen species
Hei, T. K., Liu, S. X., & Waldren, C. A. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.14.8103 · Cited by 422
Self-citationLynn, S., Gurr, J.-R., Lai, H., & Jan, K.-Y. (2000) · Circulation Research · doi:10.1161/01.res.86.5.514 · Cited by 321
The Role of Thiols in Cellular Response to Radiation and Drugs
Biaglow, J. E., Varnes, M. E., Clark, E. P., & Epp, E. R. (1983) · Radiation Research · doi:10.2307/3576092 · Cited by 255
Yin, B., Whyatt, R. M., Perera, F. P., Randall, M. C., Cooper, T. B., & Santella, R. M. (1995) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(95)00003-g · Cited by 251
Self-citation
Research works printed but not linked: 11
- Axelson O: Arsenic compounds and cancer. In: V. Vainio (eds). Occupational Cancer and Carcinogenesis. Hemisphere Publishing Corporation, Washington, D.C., 1980, pp 1229–1235.
- U.S. EPA: Soil Screening Guidance Technical Background Document. Office of Solid Waste and Emergency Response. EPA/540/R-95/128. United States Environmental Protection Agency, Washington, D.C., 1996
- Dhar RK, Biswas BK, Samanta G: Groundwater arsenic calamity in Bangladesh. Curr Sci 73: 48–59, 1997
- Warner ML, Moore LE, Smith MT, Kalman DA, Fanning E, Smith AH: Increased micronuclei in exofoliated bladder cells of individuals who chronically ingested arsenic-contaminated water in Nevada. Cancer Epidem Biomarkers Prevent 3: 583–590, 1994
- IARC: Monograph on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. Suppl. 4, 1982, pp 50–51
- Wang Z, Rossman TG: The carcinogenicity of arsenic. In: T. Chang (eds). Toxicology of Metals. CRC Press, Boca Raton, 1996, pp 221–229
- Hei TK, Piao CQ, He ZY, Vannais D, Waldren CA: Chrysotile fiber is a potent mutagen is mammalian cells. Cancer Res 52: 6305–6309, 1992
- Xu A, Wu LJ, Santella R, Hei, TK: Role of reactive oxygen species in the mutagenicity and DNA damage induced by crocidolite fibers in mammalian cells Cancer Res 59: 5615–5624, 1999
- Yarborough A, Zhang UJ, Hsu TM, Santella RM: Immunoperoxidase detection of 8-OHdG in aflatoxin B1-treated rat liver and human oral mucosal cells. Cancer Res 56: 683–688, 1996
- Applegate LA, Luscher P, Tyrrell RM: Induction of heme oxygenase: A general response of oxidant stress in cultured mammalian cells. Cancer Res 51: 974–978, 1991
- Engel RR, Hopenhayn-Rich C, Receveur O, Smith AH: Vascular effects of chronic arsenic exposure. Epidemiol Res 16: 184–208, 1994
Cited by
Sorted by citations
Heavy metals: toxicity and human health effects
Jomová, K., Alomar, S. Y., Nepovimová, E., Kuča, K., & Valko, M. (2024) · Archives of Toxicology · doi:10.1007/s00204-024-03903-2 · Cited by 1,229
A review on detection of heavy metal ions in water—An electrochemical approach
Gumpu, M. B., Sethuraman, S., Krishnan, U. M., & Rayappan, J. B. B. (2015) · Sensors and Actuators B: Chemical · doi:10.1016/j.snb.2015.02.122 · Cited by 1,027
Arsenic — a Review. Part I: Occurrence, Toxicity, Speciation, Mobility
Bissen, M., & Frimmel, F. H. (2003) · Acta hydrochimica et hydrobiologica · doi:10.1002/aheh.200390025 · Cited by 916
Mechanism of arsenic carcinogenesis: an integrated approach
Rossman, T. G. (2003) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2003.07.009 · Cited by 566
Cellular responses to nanoparticles: Target structures and mechanisms
Unfried, K., Albrecht, C., Klotz, L., von Mikecz, A. H., Grether‐Beck, S., & Schins, R. P. F. (2007) · Nanotoxicology · doi:10.1080/00222930701314932 · Cited by 507
Metadata
- Authors
- Kessel, M., Liu, S. X., Xu, A., Santella, R., & Hei, T. K.
- Affiliations
- Columbia University (US)
- Corresponding author
- Hei, Tom K.
- Venue
- Molecular and Cellular Biochemistry
- Published
- 2002-05
- Volume
- 234-235
- Issue
- 1
- Pages
- 301-308
- ISSN
- 0300-8177 (ISSN-L)
- 1573-4919
- OpenAlex
- W4243944514
- Cited by
- 135
- References
- 30
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 9 · 2020: 3 · 2019: 5 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 5 · 2014: 9 · 2013: 7 · 2012: 9
- FWCI
- 3.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Molecular and Cellular Biochemistry (publishedVersion)
- Funders
- U.S. Environmental Protection Agency
- National Institutes of Health
- National Institute of Environmental Health Sciences (P42 ES10349, ES 08821, P30-ES09089)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Physical Sciences › Environmental Science › Environmental Chemistry › Arsenic contamination and mitigation
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Keywords
- arsenic, arsenite, oxidative DNA damage, genotoxicity, carcinogenesis, reactive oxygen species, superoxide dismutase, catalase, antioxidant enzymes, 8-hydroxy-2'-deoxyguanosine, glutathione, buthionine sulfoximine, cytotoxicity, mutagenicity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
