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Cited by 48Marine and coastal plant biologyPhotosynthetic Processes and MechanismsEnzyme function and inhibition

The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.

External HCO3 − dehydration maintained by acid zones in the plasma membrane is an important component of the photosynthetic carbon uptake in Ruppia cirrhosa

Hellblom, Frida, Axelsson, Lennart·2003·Photosynthesis Research
DOI 10.1023/a:1025809415048
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Abstract

Ruppia cirrhosa, a temperate seagrass growing in brackish water, featured a high capacity for HCO(3) (-) utilisation, which could operate over a wide pH range (from 7.5 up to 9.5) with maintained efficiency. Tris buffer inhibited this means of HCO(3) (-) utilisation in a competitive manner, while addition of acetazolamide, an inhibitor of extracellular carbonic anhydrase activity, caused a 40-50% inhibition. A mechanism involving periplasmic carbonic anhydrase-catalysed HCO(3) (-) dehydration in acid zones, followed by a (probably diffusive) transport of the formed CO(2) across the plasma membrane was thus, at least partly, responsible for the HCO(3) (-) utilisation. This mechanism, which comprises a CO(2)-concentrating mechanism (CCM) associated with the plasma membrane, is thus shown for the first time in an aquatic angiosperm. Additional mechanisms involved in the Tris-sensitive HCO(3) (-) utilisation could be direct HCO(3) (-) uptake (e.g., in an H(+)/HCO(3) (-)symport) or (more likely) non-catalysed HCO(3) (-) dehydration in the acid zones. Based on these results, and on earlier investigations on Zostera marina, a general model for analysis of HCO(3) (-) utilisation mechanisms of seagrasses is suggested. In this model, three 'systems' for HCO(3) (-) utilisation are defined which are characterised (and can to some extent be quantified) by their capability to operate at high pH in combination with their response to acetazolamide and Tris. Some consequences of the fact that HCO(3) (-) utilisation and osmoregulation probably depend on the same energy source (ATP via H(+)-ATPase in the plasma membrane) are discussed.

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Research works printed but not linked: 3

  1. Hellblom F, Moulin P, Björk M and Axelsson L (2002) Buffer sensitive carbon acquisition systems in submerged angiosperms - examples from marine and brackish water habitat. In: Hellblom F, Mechanisms of inorganic carbon acquisition in marine angiosperms (seagrasses). PhD thesis, Dept of Botany, Stockholm University
  2. Pytkowicz RM, Atlas E and Culberson CH (1977) Oceanography and Marine Biology. Ann Rev 15: 11–45
  3. Spence DHN and Maberly SC (1985) Occurrence and ecological importance of HCO3¯ use among aquatic higher plants. In: Lucas WJ and Berry J (eds) Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, pp 125–143. American Society of Plant Physiologists, Rockville, Maryland

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Metadata

Authors
Hellblom, F., & Axelsson, L.
Affiliations
Södertörn University (SE)
Corresponding author
Hellblom, Frida
Venue
Photosynthesis Research
Publisher
Springer Science and Business Media LLC
Published
2003-08
Volume
77
Issue
2-3
Pages
173-181
ISSN
  • 0166-8595 (ISSN-L)
  • 1573-5079
DOI
10.1023/a:1025809415048
OpenAlex
W1507304153
Cited by
48
References
34
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 2 · 2019: 3 · 2018: 5 · 2017: 4 · 2016: 3 · 2015: 3 · 2013: 1 · 2012: 2
FWCI
1.84
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Photosynthesis Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme function and inhibition
Keywords
Ruppia cirrhosa, carbonic anhydrase, acetazolamide inhibition, Tris buffer, CO2-concentrating mechanism, photosynthetic carbon uptake, seagrass, osmoregulation, H+-ATPase, Zostera marina

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