The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
External HCO3 − dehydration maintained by acid zones in the plasma membrane is an important component of the photosynthetic carbon uptake in Ruppia cirrhosa
Abstract
Ruppia cirrhosa, a temperate seagrass growing in brackish water, featured a high capacity for HCO(3) (-) utilisation, which could operate over a wide pH range (from 7.5 up to 9.5) with maintained efficiency. Tris buffer inhibited this means of HCO(3) (-) utilisation in a competitive manner, while addition of acetazolamide, an inhibitor of extracellular carbonic anhydrase activity, caused a 40-50% inhibition. A mechanism involving periplasmic carbonic anhydrase-catalysed HCO(3) (-) dehydration in acid zones, followed by a (probably diffusive) transport of the formed CO(2) across the plasma membrane was thus, at least partly, responsible for the HCO(3) (-) utilisation. This mechanism, which comprises a CO(2)-concentrating mechanism (CCM) associated with the plasma membrane, is thus shown for the first time in an aquatic angiosperm. Additional mechanisms involved in the Tris-sensitive HCO(3) (-) utilisation could be direct HCO(3) (-) uptake (e.g., in an H(+)/HCO(3) (-)symport) or (more likely) non-catalysed HCO(3) (-) dehydration in the acid zones. Based on these results, and on earlier investigations on Zostera marina, a general model for analysis of HCO(3) (-) utilisation mechanisms of seagrasses is suggested. In this model, three 'systems' for HCO(3) (-) utilisation are defined which are characterised (and can to some extent be quantified) by their capability to operate at high pH in combination with their response to acetazolamide and Tris. Some consequences of the fact that HCO(3) (-) utilisation and osmoregulation probably depend on the same energy source (ATP via H(+)-ATPase in the plasma membrane) are discussed.
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“Extracellular CA-mediation of HCO3- uptake is less likely to depend on light availability, when compared to the H+ extrusion-driven co-transport of HCO3- [26, 29].”
From Light Levels Affect Carbon Utilisation in Tropical Seagrass under Ocean Acidification (2016) · cites this paper as [29]
Citations
Cites
Phylogenetic Studies in Alismatidae, II: Evolution of Marine Angiosperms (Seagrasses) and Hydrophily
Les, D. H., Cleland, M. A., & Waycott, M. (1997) · Systematic Botany · doi:10.2307/2419820 · Cited by 441
Inorganic Carbon Acquisition by Marine Autotrophs
RAVEN, J. (1997) · Advances in botanical research · doi:10.1016/s0065-2296(08)60281-5 · Cited by 304
Carbon dioxide hydration and dehydration kinetics in seawater1
Johnson, K. S. (1982) · Limnology and Oceanography · doi:10.4319/lo.1982.27.5.0849 · Cited by 287
Moroney, J. V., Husic, H. D., & Tolbert, N. E. (1985) · PLANT PHYSIOLOGY · doi:10.1104/pp.79.1.177 · Cited by 264
Photosynthetic Assimilation of Exogenous HCO3 by Aquatic Plants
Lucas, W. J. (1983) · Annual Review of Plant Physiology · doi:10.1146/annurev.pp.34.060183.000443 · Cited by 220
Differential ability of marine and freshwater macrophytes to utilize HCO 3 - and CO2
Sand‐Jensen, K., & Gordon, D. M. (1984) · Marine Biology · doi:10.1007/bf00392819 · Cited by 147
Two modes of bicarbonate utilization in the marine green macroalga Ulva lactuca
Axelsson, L., Ryberg, H., & Beer, S. (1995) · Plant Cell & Environment · doi:10.1111/j.1365-3040.1995.tb00378.x · Cited by 138
Self-citationWalker, N. A., SMITH, F. A., & Cathers, I. (1980) · The Journal of Membrane Biology · doi:10.1007/bf01868985 · Cited by 134
Inorganic carbon utilization in marine angiosperms (seagrasses)
Beer, S., Bjork, M., Hellblom, F., & Axelsson, L. (2002) · Australian Journal of Plant Physiology · doi:10.1071/pp01185 · Cited by 131 · cited 2×
“Much of the early work in this field was done on characeans and freshwater angiosperms (Lucas 1983; Spence and Maberly 1985), but later also on microand macroalgae and marine angiosperms (Raven 1997; Axelsson and Beer 2001; Beer et al. 2002).”
BackgroundSelf-citationProton Gradients and Plant Growth: Role of the Plasma Membrane H+-ATPase
Palmgren, M. B. (1998) · Advances in botanical research · doi:10.1016/s0065-2296(08)60293-1 · Cited by 130
Research works printed but not linked: 3
- Hellblom F, Moulin P, Björk M and Axelsson L (2002) Buffer sensitive carbon acquisition systems in submerged angiosperms - examples from marine and brackish water habitat. In: Hellblom F, Mechanisms of inorganic carbon acquisition in marine angiosperms (seagrasses). PhD thesis, Dept of Botany, Stockholm University
- Pytkowicz RM, Atlas E and Culberson CH (1977) Oceanography and Marine Biology. Ann Rev 15: 11–45
- Spence DHN and Maberly SC (1985) Occurrence and ecological importance of HCO3¯ use among aquatic higher plants. In: Lucas WJ and Berry J (eds) Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, pp 125–143. American Society of Plant Physiologists, Rockville, Maryland
Cited by
Sorted by citations
Climate change and ocean acidification effects on seagrasses and marine macroalgae
Koch, M., Bowes, G., Ross, C., & Zhang, X. (2013) · Global Change Biology · doi:10.1111/j.1365-2486.2012.02791.x · Cited by 869
“…/H+ co-transport has been reported for the seagrasses Halophila ovalis and Ruppia cirrhosa using indirect inhibitor methods, but the acidification zones were not characterized, although a bipolar leaf scenario seems possible (Beer et al., 2002, 2006; Hellblom & Axelsson, 2003; Uku et al., 2005).”
BackgroundMethodThe evolution of inorganic carbon concentrating mechanisms in photosynthesis
Raven, J. A., Cockell, C. S., & Rocha, C. L. D. L. (2008) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2008.0020 · Cited by 337
Ries, J. B. (2010) · Biogeosciences · doi:10.5194/bg-7-2795-2010 · Cited by 278
“In one model, H+ ions complex with HCO−3 ions within the algae’s interutricular space, thus facilitating CO2 extraction by dehydration of H2CO3 within the algal cell (McConnaughey and Whelan, 1997; Hellblom et al., 2001; Hellblom and Axelsson, 2003).”
BackgroundTransport and Use of Bicarbonate in Plants: Current Knowledge and Challenges Ahead
Poschenrieder, C., Fernández, J. A., Rubio, L., Pérez‐Martín, L., Terés, J., & Barceló, J. A. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19051352 · Cited by 144
“Such a mechanism has been proposed for Zostera marina [88,93,94], Zostera noltii [95], Posidonia oceanica and Cymodocea nodosa [96], Halophila stipulacea and Ruppia maritima [96], Ruppia cirrhosa [97], and Halophila ovalis [98].”
BackgroundPhotosynthetic response to globally increasing CO2 of co‐occurring temperate seagrass species
Borum, J., Pedersen, O., Kotula, L., Fraser, M. W., Statton, J., Colmer, T. D., & Kendrick, G. A. (2015) · Plant Cell & Environment · doi:10.1111/pce.12658 · Cited by 74
Metadata
- Authors
- Hellblom, F., & Axelsson, L.
- Affiliations
- Södertörn University (SE)
- Corresponding author
- Hellblom, Frida
- Venue
- Photosynthesis Research
- Published
- 2003-08
- Volume
- 77
- Issue
- 2-3
- Pages
- 173-181
- ISSN
- 0166-8595 (ISSN-L)
- 1573-5079
- OpenAlex
- W1507304153
- Cited by
- 48
- References
- 34
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 2 · 2019: 3 · 2018: 5 · 2017: 4 · 2016: 3 · 2015: 3 · 2013: 1 · 2012: 2
- FWCI
- 1.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Photosynthesis Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme function and inhibition
- Keywords
- Ruppia cirrhosa, carbonic anhydrase, acetazolamide inhibition, Tris buffer, CO2-concentrating mechanism, photosynthetic carbon uptake, seagrass, osmoregulation, H+-ATPase, Zostera marina
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