The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Role of Rab5 in the Recruitment of hVps34/p150 to the Early Endosome
Abstract
PI 3‐kinases are important regulators of endocytic trafficking. We have previously proposed a model in which the Rab5 GTPase recruits EEA1 to the early endosome both directly, by binding to EEA1, and indirectly, through the recruitment of the p150/hVps34 PI 3‐kinase and the production of PI[3]P in the endosomal membrane. In this study we have examined this model in vivo . We find that both endogenous hVps34 and p150 are targeted to enlarged endosomal structures in cells expressing constitutively activated Rab5, where they are significantly colocalized with EEA1. Recombinant fragments of p150 disrupt the endosomal localization of EEA1, showing that p150 is required for EEA1 targeting. We further analyzed the mechanism of GTP‐dependent Rab5‐p150 binding, and showed the p150 HEAT and WD40 domains are required for binding, whereas deletion of the protein kinase domain increases binding to Rab5. Overexpression of constitutively active Rab5 caused a redistribution of epitope‐tagged hVps34 and p150 to Rab5‐positive endosomes. However, subcellular fractionation showed that this was not due to a significant recruitment of hVps34 or p150 from the cytosolic to the particulate fraction. These data suggest that the binding of Rab5 to the HEAT/WD40 domains of p150 is important in regulating the localization of hVps34/p150. However, Rab5 does not appear to act by directly recruiting p150/hVps34 complexes from the cytosol to the endosomal membrane.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
VPS34 is the main lipid kinase involved in the generation of endosomal PI(3)P.
“VPS34, the main lipid kinase involved in the generation of endosomal PI(3)P2–4”
From Endosomal PI(3)P regulation by the COMMD/CCDC22/CCDC93 (CCC) complex controls membrane protein recycling (2019) · cites this paper as [4]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Kihara, A., Noda, T., Ishihara, N., & Ohsumi, Y. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.152.3.519 · Cited by 1,004
McBride, H. M., Rybin, V., Murphy, C., Giner, A., Teasdale, R., & Zerial, M. (1999) · Cell · doi:10.1016/s0092-8674(00)81966-2 · Cited by 520
Lawe, D. C., Chawla, A., Merithew, E., Dumas, J., Carrington, W., Fogarty, K., Lifshitz, L., Tuft, R., Lambright, D., & Corvera, S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m109239200 · Cited by 156
Flagged first, then by citations
Research works printed but not linked: 42
- 10.1152/physrev.1997.77.3.759 · DOI
- 10.1128/MCB.13.10.6052 · DOI
- 10.1002/j.1460-2075.1994.tb06747.x · DOI
- 10.1074/jbc.273.27.17128 · DOI
- 10.1016/S0092-8674(00)81701-8 · DOI
- 10.1016/S0960-9822(99)80422-9 · DOI
- 10.1074/jbc.273.9.4811 · DOI
- 10.1016/0955-0674(95)80013-1 · DOI
- 10.1006/mcbr.2000.0202 · DOI
- 10.1038/35052055 · DOI · Paper page
- 10.1038/28879 · DOI · Paper page
- 10.1046/j.1432-1327.1999.00743.x · DOI
- 10.1038/12075 · DOI · Paper page
- 10.1016/S0955-0674(99)80066-0 · DOI
- 10.1242/jcs.112.23.4175 · DOI
- 10.1073/pnas.94.14.7326 · DOI
- SiddhantaU McIlroyJ ShahA ZhangY BackerJM.Distinct roles for the p110alpha and hVPS34 phosphatidylinositol 3‐kinases in vesicular trafficking regulation of the actin cytoskeleton and mitogenesisJ Cell Biol1998;143:1647–1659. · DOI
- 10.1016/s0092-8674(01)00414-7 · DOI
- 10.1002/j.1460-2075.1993.tb05867.x · DOI
- 10.1074/jbc.272.4.2477 · DOI
- 10.1083/jcb.143.6.1647 · DOI
- 10.1002/j.1460-2075.1994.tb06381.x · DOI · Paper page
- 10.1093/emboj/17.7.1930 · DOI
- 10.1128/MCB.10.12.6742 · DOI
- 10.1016/0092-8674(91)90650-N · DOI
- 10.1038/28771 · DOI
- 10.1074/jbc.275.5.3699 · DOI
- 10.1016/S0959-440X(99)80052-9 · DOI
- 10.1006/jmbi.2001.4624 · DOI
- 10.1016/S0092-8674(00)80963-0 · DOI · Paper page
- Cassimeris L, 2001, XMAP215 is a long thin molecule that does not increase microtubule stiffness, J Cell Sci, 114, 3025, 10.1242/jcs.114.16.3025 · DOI
- 10.1038/20367 · DOI
- 10.1101/gr.147400 · DOI
- 10.1093/nar/28.1.231 · DOI
- 10.1073/pnas.95.11.5857 · DOI
- 10.1016/S0968-0004(99)01384-5 · DOI
- 10.1038/379369a0 · DOI
- 10.1242/jcs.114.12.2255 · DOI
- 10.1074/jbc.M109263200 · DOI
- 10.1083/jcb.149.4.901 · DOI · Paper page
- 10.1002/j.1460-2075.1996.tb00602.x · DOI
- 10.1093/oxfordjournals.jbchem.a002964 · DOI
Cited by
Sorted by citations
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
Self-citationRab Proteins and the Compartmentalization of the Endosomal System
Wandinger-Ness, A., & Zerial, M. (2014) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a022616 · Cited by 586
Endocytosis in the context-dependent regulation of individual and collective cell properties
Sigismund, S., Lanzetti, L., Scita, G., & Di Fiore, P. P. (2021) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-021-00375-5 · Cited by 132
Singla, A., Fedoseienko, A., Giridharan, S. S. P., Overlee, B. L., Lopez, A., Jia, D., Song, J., Huff-Hardy, K., Weisman, L., Burstein, E., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-12221-6 · Cited by 113
Myotubularin Lipid Phosphatase Binds the hVPS15/hVPS34 Lipid Kinase Complex on Endosomes
Cao, C., Laporte, J., Backer, J. M., Wandinger‐Ness, A., & Stein, M. (2007) · Traffic · doi:10.1111/j.1600-0854.2007.00586.x · Cited by 92
Self-citationNRBF2 regulates macroautophagy as a component of Vps34 Complex I
Cao, Y., Wang, Y., Abi Saab, W. F., Yang, F., Pessin, J. E., & Backer, J. M. (2014) · Biochemical Journal · doi:10.1042/bj20140515 · Cited by 84
Self-citationWhite, J. A., Anderson, E., Zimmerman, K., Zheng, K. H., Rouhani, R., & Gunawardena, S. (2015) · Human Molecular Genetics · doi:10.1093/hmg/ddv415 · Cited by 67
Metadata
- Authors
- Murray, J. T., Panaretou, C., Stenmark, H., Miaczynska, M., & Backer, J. M.
- Venue
- Traffic
- Publisher
- Wiley
- Published
- 2002-05-09
- Volume
- 3
- Issue
- 6
- Pages
- 416-427
- ISSN
- 1398-9219
- 1600-0854
- Cited by
- 185
- References
- 3
- Language
- en
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
