Novelty, but not operant aversive learning, enhances Fos and Egr-1 expression in the medial prefrontal cortex and hippocampal areas of rats
Abstract
Immediate early genes (IEG) are presumed to be activated in response to stress, novelty, and learning. Evidence supports the involvement of prefrontal and hippocampal areas in stress and learning, but also in the detection of novel events. This study examined whether a previous experience with shocks changes the pattern of Fos and Egr-1 expression in the medial prefrontal cortex (mPFC), the hippocampal cornus ammonis 1 (CA1), and dentate gyrus (DG) of adult male Wistar rats that learned to escape in an operant aversive test. Subjects previously exposed to inescapable footshocks that learned to escape from shocks were assigned to the treated group (EXP). Subjects from Group Novelty (NOV) rested undisturbed during treatment and also learned to escape in the test. The nonshock group (NSH) rested undisturbed in both sessions. Standard immunohistochemistry procedures were used to detect the proteins in brain sections. The results show that a previous experience with shocks changed the pattern of IEG expression, then demonstrating c-fos and egr-1 induction as experience-dependent events. Compared with NSH and EXP an enhanced Fos expression was detected in the mPFC and CA1 subfield of Group NOV, which also exhibited increased Egr-1 expression in the mPFC and DG in comparison to NSH. No differences were found in the DG for Fos, or in the CA1 for Egr-1. Novelty, and not the operant aversive escape learning, seems to have generated IEG induction. The results suggest novel stimuli as a possible confounding factor in studies on Fos and/or Egr-1 expression in aversive conditions.
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EGR1 has been shown to be useful as an indirect marker of neuronal activation in a variety of species and contexts.
“EGR1 has been shown to be useful as an indirect marker of neuronal activation in a variety of species and contexts (28–30).”
From Oxytocin receptors in the anteromedial bed nucleus of the stria terminalis promote stress-induced social avoidance in female California mice (2018) · cites this paper as [30]
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- Authors
- Yochiy, A., Britto, L. R. G., & Hunziker, M. H. L.
- Affiliations
- Universidade de São Paulo (BR)
- Venue
- Behavioral Neuroscience
- Published
- 2012-12
- Volume
- 126
- Issue
- 6
- Pages
- 826-834
- ISSN
- 0735-7044 (ISSN-L)
- 1939-0084
- OpenAlex
- W2332145953
- Cited by
- 16
- References
- 13
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 4 · 2016: 1 · 2014: 2 · 2013: 1
- FWCI
- 0.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Behavioral Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Scientific Electronic Library Online (São Paulo Research Foundation, Latin American and Caribbean Center on Health Sciences Information, Conselho Nacional de Desenvolvimento Científico e Tecnológico) (publishedVersion)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- novelty, FOS, EGR1, immediate early genes, medial prefrontal cortex, hippocampus, CA1, dentate gyrus, footshock, stress
- MeSH terms
- Animals, Hippocampus, Learning, Male, Transcription Factors, Proto-Oncogene Proteins c-fos, Rats, Wistar, Prefrontal Cortex, Dentate Gyrus, Rats, Early Growth Response Protein 1
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