Cited by 4,683Advanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsRedox biology and oxidative stress

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Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

Gygi, Steven P., Rist, Beate, Gerber, Scott A., Turecek, Frantisek, Gelb, Michael H., Aebersold, Ruedi·1999·Nature Biotechnology

DOI 10.1038/13690

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Abstract

We describe an approach for the accurate quantification and concurrent sequence identification of the individual proteins within complex mixtures. The method is based on a class of new chemical reagents termed isotope-coded affinity tags (ICATs) and tandem mass spectrometry. Using this strategy, we compared protein expression in the yeast Saccharomyces cerevisiae, using either ethanol or galactose as a carbon source. The measured differences in protein expression correlated with known yeast metabolic function under glucose-repressed conditions. The method is redundant if multiple cysteinyl residues are present, and the relative quantification is highly accurate because it is based on stable isotope dilution techniques. The ICAT approach should provide a widely applicable means to compare quantitatively global protein expression in cells and tissues.

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“Cysteine is also severely under-represented in these high-scoring peptides, and this fact suggests that the cysteine-containing peptides isolated using specific enrichment methods [45] may not produce the best fragmentation.”
Background
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Method
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“Alternatively, the stable isotopes can be incorporated invitro, chemically [9, 13, 14] or enzymatically such as”
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“ICAT has been most successful in the quantitative proteomic study of regulatory processes in yeast (11), in which very large numbers of cells can be harvested.”
BackgroundMethodInfluential
Stable isotope methods for high-precision proteomics
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Authors: Gygi, S. P., Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H., & Aebersold, R.
Affiliations: University of Washington (US)
Corresponding author: Aebersold, Ruedi
ORCID: Gygi, Steven P.
Gerber, Scott A.
Gelb, Michael H.
Aebersold, Ruedi
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 1999-10
Volume: 17
Issue: 10
Pages: 994-999
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/13690
OpenAlex: W2111208057
Semantic Scholar: 8929f01bc9a788127bcff7a09432f4f7979c8e6b
Cited by: 4,683
References: 24
Citations by year: 2026: 19 · 2025: 27 · 2024: 31 · 2023: 54 · 2022: 50 · 2021: 63 · 2020: 51 · 2019: 70 · 2018: 77 · 2017: 104 · 2016: 123 · 2015: 171 · 2014: 188 · 2013: 235 · 2012: 296
FWCI: 75.18
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
Funders: National Human Genome Research Institute (T32 HG-00035, T32HG000035)
National Center for Research Resources (RR-11823, P41RR011823)
Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 02274, P30HD002274)
Topics: Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords: ICAT, protein quantification, tandem mass spectrometry, protein expression profiling, Saccharomyces cerevisiae, glucose repression, ethanol carbon source, stable isotope dilution, cysteine labeling
MeSH terms: Affinity Labels, Amino Acid Sequence, Chromatography, Liquid, Isotope Labeling, Proteins, Mass Spectrometry

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Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

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