Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

Gygi, Steven P., Rist, Beate, Gerber, Scott A., Turecek, Frantisek, Gelb, Michael H., Aebersold, Ruedi·1999·Nature Biotechnology

Cited by (1,009)

Items 201–300 of 1,009

  1. All about DIGE: quantification technology for differential-display 2D-gel proteomics
    Kathryn Susan Lilley, David B. Friedman · 2004 · Expert Review of Proteomics
  2. Chemical combination effects predict connectivity in biological systems
    Lehár, Joseph, Zimmermann, Grant R, Krueger, Andrew S, Molnar, Raymond A, Ledell, Jebediah T, Heilbut, Adrian M, Short, Glenn F, Giusti, Leanne C, Nolan, Garry P, Magid, Omar A et al. · 2007 · Molecular Systems Biology
  3. Mass spectrometry and proteomics
    Steven P. Gygi, Ruedi H. Aebersold · 2000 · Current Opinion in Chemical Biology
  4. Time-course metabolic profiling in Arabidopsis thaliana cell cultures after salt stress treatment*
    Jae Kwang Kim, Takeshi Bamba, Kazuo Harada, Eiichiro Fukusaki, Akio Kobayashi · 2006 · Journal of Experimental Botany
  5. An Improved Model for Prediction of Retention Times of Tryptic Peptides in Ion Pair Reversed-phase HPLC
    Krokhin, O.V., Craig, R., Spicer, V., Ens, W., Standing, K.G., Beavis, R.C., Wilkins, J.A. · 2004 · Molecular & Cellular Proteomics
  6. OMICS-driven biomarker discovery in nutrition and health
    Martin Kussmann, Frédéric Raymond, Michael Affolter · 2006 · Journal of Biotechnology
  7. Solid-phase extraction of N-linked glycopeptides
    Yuan Tian, Yong Feng Zhou, Sarah Elliott, Ruedi H. Aebersold, Hui Zhang · 2007 · Nature Protocols
  8. Selected reaction monitoring applied to proteomics
    Sébastien Gallien, Elodie Duriez, Bruno Domon · 2011 · Journal of Mass Spectrometry
  9. Organelle Proteomics
    Lukas Alfons Huber, Kristian Pfaller, Ilja Vietor · 2003 · Circulation Research
  10. Use of mass spectrometry to identify protein biomarkers of disease severity in the synovial fluid and serum of patients with rheumatoid arthritis.
    H. Liao, Jiang Wu, Eric Kuhn, Wendy Chin, Betty Chang, Michael D. Jones, Steve O'Neil, K. Clauser, J. Karl, F. Hasler et al. · 2004 · Arthritis & Rheumatism
  11. Quantitative Mass Spectrometry-Based Proteomics: An Overview
    Svitlana Rozanova, Katalin Barkovits, Miroslav Nikolov, Carla Schmidt, Henning Urlaub, Katrin Marcus · 2021 · Methods in molecular biology
  12. i-Tracker: For quantitative proteomics using iTRAQ™
    Ian Shadforth, Tom P. J. Dunkley, Kathryn Susan Lilley, Conrad Bessant · 2005 · BMC Genomics
  13. Differential stable isotope labeling of peptides for quantitation and de novo sequence derivation
    David R. Goodlett, Andrew Keller, Julian D. Watts, Richard A. Newitt, Eugene C. Yi, Samuel Owen Purvine, Jimmy K. Eng, Priska D. von Haller, Ruedi H. Aebersold, Eugene Kolker · 2001 · Rapid Communications in Mass Spectrometry
  14. Bioinformatics Methods for Mass Spectrometry-Based Proteomics Data Analysis
    Chen, Chen, Hou, Jie, Tanner, John J., Cheng, Jianlin · 2020 · International Journal of Molecular Sciences
  15. Differential Protein Expression Profiling by iTRAQ−2DLC−MS/MS of Lung Cancer Cells Undergoing Epithelial-Mesenchymal Transition Reveals a Migratory/Invasive Phenotype
    Venkateshwar G. Keshamouni, George C. Michailidis, Catherine S. Grasso et al. · 2006 · Journal of Proteome Research
  16. Chemical Glycoproteomics
    Krishnan K. Palaniappan, Carolyn Ruth Bertozzi · 2016 · Chemical Reviews
  17. Measuring and Managing Ratio Compression for Accurate iTRAQ/TMT Quantification
    Mikhail M. Savitski, Toby Mathieson, Nico Zinn, Gavain M. A. Sweetman, Carola Doce, Isabelle Becher, Fiona Pachl, Bernhard Küster, Marcus Bantscheff · 2013 · Journal of Proteome Research
  18. Membrane protease proteomics: Isotope-coded affinity tag MS identification of undescribed MT1–matrix metalloproteinase substrates
    Eric M. Tam, Charlotte Morrison, Yi Wu, Mary Sharon Stack, Christopher M. Overall · 2004 · Proceedings of the National Academy of Sciences
  19. O-GlcNAc profiling: from proteins to proteomes
    Junfeng Ma, Gerald Warren Hart · 2014 · Clinical Proteomics
  20. A high-quality catalog of the Drosophila melanogaster proteome
    Erich Brunner, Christian H. Ahrens, Sonali Mohanty, Hansruedi Baetschmann, Sandra Nicola Lövenich, Frańk Potthast, Eric W. Deutsch, Christian Panse, Ulrik de Lichtenberg, Oliver Rinner et al. · 2007 · Nature Biotechnology
  21. Current trends in quantitative proteomics
    Monica H. Elliott, DEREK S. H. SMITH, Carol E. Parker, Christoph H. Borchers · 2009 · Journal of Mass Spectrometry
  22. Complementary Proteome and Transcriptome Profiling in Phosphate-deficient Arabidopsis Roots Reveals Multiple Levels of Gene Regulation
    Ping Lan, Wenfeng Li, Wolfgang Schmidt · 2012 · Molecular & Cellular Proteomics
  23. Antibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers
    Jeffrey R. Whiteaker, Lei Zhao, Heidi Y. Zhang · 2006 · Analytical Biochemistry
  24. Cancer biomarkers: knowing the present and predicting the future
    Sabarni K. Chatterjee, Bruce R. Zetter · 2005 · Future Oncology
  25. Mass spectrometry: from proteomics to metabolomics and lipidomics
    William James Griffiths, Yuqin Wang · 2009 · Chemical Society Reviews
  26. Proteomics for cancer biomarker discovery.
    Pothur R. Srinivas, Mukesh Verma, Yinming Zhao, Sudhir Srivastava · 2002 · PubMed
  27. Application of Proteomics in Cancer: Recent Trends and Approaches for Biomarkers Discovery
    Yang Woo Kwon, Han-Seul Jo, Sungwon Bae, Youngsuk Seo, Parkyong Song, Minseok Song, Jong Hyuk Yoon · 2021 · Frontiers in Medicine
  28. Two-dimensional gel electrophoresis: recent advances in sample preparation, detection and quantitation
    Kathryn Susan Lilley, Azam Razzaq, Paul Dupree · 2002 · Current Opinion in Chemical Biology
  29. Difference gel electrophoresis
    John F. Timms, Rainer Cramer · 2008 · PROTEOMICS
  30. Mass Spectrometry Based Glycoproteomics—From a Proteomics Perspective
    Sheng Pan, Ru Chen, Ruedi H. Aebersold, Teresa A. Brentnall · 2010 · Molecular & Cellular Proteomics
  31. Aptamer Directly Evolved from Live Cells Recognizes Membrane Bound Immunoglobin Heavy Mu Chain in Burkitt's Lymphoma Cells
    Prabodhika R. Mallikaratchy, Zhiwen Tang, Kwame Sefah, Ling Hua Meng, Dihua Shangguan, Weihong Tan · 2007 · Molecular & Cellular Proteomics
  32. Protein thiol oxidation in health and disease: Techniques for measuring disulfides and related modifications in complex protein mixtures
    Philip Eaton · 2006 · Free Radical Biology and Medicine
  33. Stable isotope labelling methods in mass spectrometry-based quantitative proteomics
    Osama Chahrour, Diego Cobice, John Malone · 2015 · Journal of Pharmaceutical and Biomedical Analysis
  34. Proteomic techniques for characterisation of mesenchymal stem cell secretome
    Helena Kupcová Skalníková · 2013 · Biochimie
  35. Simultaneous Qualitative and Quantitative Analysis of theEscherichia coli Proteome
    Jeffrey C. Silva, Richard Denny, Craig A. Dorschel, Marc V. Gorenstein, Guozhong Li, Keith Richardson, Daniel Wall, Scott J. Geromanos · 2006 · Molecular & Cellular Proteomics
  36. Systems biology: integrating technology, biology, and computation
    Leroy Hood · 2003 · Mechanisms of Ageing and Development
  37. A suite of algorithms for the comprehensive analysis of complex protein mixtures using high-resolution LC-MS
    Matthew Bellew, Marc Coram, Matthew Fitzgibbon, Mark Igra, Tim R Randolph, Pei Wang, Damon May, Jimmy K. Eng, Ruihua Fang, Chenwei Lin et al. · 2006 · Bioinformatics
  38. Perspectives for mass spectrometry and functional proteomics
    Jasminka Godovac‐Zimmermann, Larry R. Brown · 2001 · Mass Spectrometry Reviews
  39. Proteomics in Genomeland
    Stanley Fields · 2001 · Science
  40. Microfluidic systems in proteomics
    Niels Lion, Tatiana C. Rohner, Loı̈c Dayon, Isabelle L. Arnaud, Eugen Damoc, Nikolay Youhnovski, Zhiyong Wu, Christophe Roussel, Jacques Josserand, Henrik M. Jensen et al. · 2003 · Electrophoresis
  41. Continuous culture – making a comeback?
    Paul A. Hoskisson, Glyn Hobbs · 2005 · Microbiology
  42. Advances in quantitative proteomics via stable isotope tagging and mass spectrometry
    W. Andy Tao, Ruedi H. Aebersold · 2003 · Current Opinion in Biotechnology
  43. Methods for samples preparation in proteomic research
    Bodzon-Kulakowska, Anna, Bierczynska-Krzysik, Anna, Dylag, Tomasz, Drabik, Anna, Suder, Piotr, Noga, Marek, Jarzebinska, Justyna, Silberring, Jerzy · 2007 · Journal of Chromatography B
  44. A Comparison of Labeling and Label-Free Mass Spectrometry-Based Proteomics Approaches
    Vibhuti J. Patel, Konstantinos Thalassinos, Susan E. Slade, Joanne B. Connolly, Andrew T. Crombie, J. Colin Murrell, James H. Scrivens · 2009 · Journal of Proteome Research
  45. Chemical Strategies for Activity‐Based Proteomics
    Anna E Speers, Benjamin F. Cravatt · 2003 · ChemBioChem
  46. MIRACLE: mass isotopomer ratio analysis of U‐13C‐labeled extracts. A new method for accurate quantification of changes in concentrations of intracellular metabolites
    Mlawule R. Mashego, Liang Wu, Jan C. van Dam, Cor Ras, Jacobus L. Vinke, Wouter A. van Winden, Walter M. van Gulik, Joseph J. Heijnen · 2004 · Biotechnology and Bioengineering
  47. Proteomic Analysis of Rat Liver Peroxisome
    Kikuchi, Miki, Hatano, Naoya, Yokota, Sadaki, Shimozawa, Nobuyuki, Imanaka, Tsuneo, Taniguchi, Hisaaki · 2004 · Journal of Biological Chemistry
  48. Accurate Protein Complex Retrieval by Affinity Enrichment Mass Spectrometry (AE-MS) Rather than Affinity Purification Mass Spectrometry (AP-MS)
    Eva C. Keilhauer, Marco Y. Hein, Matthias Mann · 2014 · Molecular & Cellular Proteomics
  49. Proteomic landscape of Alzheimer’s Disease: novel insights into pathogenesis and biomarker discovery
    Bing Bai, David Vanderwall, Yuxin Li, Xusheng Wang, Suresh Poudel, Hong Wang, Kaushik Kumar Dey, Ping‐Chung Chen, Ka Yang, Junmin Peng · 2021 · Molecular Neurodegeneration
  50. Derivatization for liquid chromatography-mass spectrometry
    Qi, Bao-Ling, Liu, Ping, Wang, Qiu-Yi, Cai, Wen-Jing, Yuan, Bi-Feng, Feng, Yu-Qi · 2014 · TrAC Trends in Analytical Chemistry
  51. MSQuant, an Open Source Platform for Mass Spectrometry-Based Quantitative Proteomics
    Peter Mortensen, Joost W. Gouw, Jesper V. Olsen, Shao‐En Ong, Kristoffer T. G. Rigbolt, Jakob Bunkenborg, Jürgen Cox, Leonard J. Foster, Albert J. R. Heck, Blagoy Blagoev et al. · 2009 · Journal of Proteome Research
  52. The Large Subunit of the Mammalian Mitochondrial Ribosome
    Emine C. Koc, William Burkhart, Kevin Blackburn, Mary B. Moyer, Daniela Schlatzer, Martin Arthur Moseley, Linda Lucy Spremulli · 2001 · Journal of Biological Chemistry
  53. Redox Proteomics: Chemical Principles, Methodological Approaches and Biological/Biomedical Promises
    Angela Bachi, Isabella Dalle‐Donne, Andrea Scaloni · 2012 · Chemical Reviews
  54. An assembly landscape for the 30S ribosomal subunit
    Megan W. T. Talkington, Gary E. Siuzdak, James R. Williamson · 2005 · Nature
  55. Multiple ligand-specific conformations of the β2-adrenergic receptor
    Alem W. Kahsai, Kunhong Xiao, Sudarshan Rajagopal, Seungkirl Ahn, Arun K. Shukla, Jin‐Peng Sun, Terrence G. Oas, Robert J. Lefkowitz · 2011 · Nature Chemical Biology
  56. A Proteomic Approach for Identification of Secreted Proteins during the Differentiation of 3T3-L1 Preadipocytes to Adipocytes
    Irina Kratchmarova, Dário Eluan Kalume, Blagoy Blagoev, Philipp E. Scherer, Alexandre V. Podtelejnikov, Henrik Molina, Perry E. Bickel, Jens Andersen, Minerva M. Fernandez, Jacob Bunkenborg et al. · 2002 · Molecular & Cellular Proteomics
  57. Proteomics Analysis of Rat Brain Postsynaptic Density
    Ka Wan Li, Martin P. Hornshaw, Roel C. van der Schors, Rod B. Watson, Stephen A. Tate, Bruno Casetta, Connie R. Jiménez, Yvonne Gouwenberg, Eckart Dieter Gundelfinger, Karl‐Heinz Smalla et al. · 2004 · Journal of Biological Chemistry
  58. A systematic approach to modeling, capturing, and disseminating proteomics experimental data
    Chris F. Taylor, Norman W. Paton, Kevin L. Garwood, Paul D. Kirby, David Stead, Zhikang Yin, Eric W. Deutsch, Laura Selway, Janet Walker, Isabel Riba‐Garcia et al. · 2003 · Nature Biotechnology
  59. Proteomic analysis of human prostasomes
    Angelita G. Utleg, Eugene C. Yi, Tao Xie, Paul T. Shannon, James T. White, David R. Goodlett, Leroy Hood, Biaoyang Lin · 2003 · The Prostate
  60. Tagging and detection strategies for activity-based proteomics
    Amir Masoud Sadaghiani, Steven H. L. Verhelst, Matthew S. Bogyo · 2006 · Current Opinion in Chemical Biology
  61. A Proteomic Strategy for Gaining Insights into Protein Sumoylation in Yeast
    Carilee Denison, Adam D. Rudner, Scott A. Gerber, Corey E. Bakalarski, Danesh Moazed, Steven P. Gygi · 2004 · Molecular & Cellular Proteomics
  62. The Small Subunit of the Mammalian Mitochondrial Ribosome
    Emine C. Koc, William Burkhart, Kevin Blackburn, Martin Arthur Moseley, Linda Lucy Spremulli · 2001 · Journal of Biological Chemistry
  63. Mass Spectrometry-Based Protein Footprinting for Higher-Order Structure Analysis: Fundamentals and Applications
    Xiaoran Roger Liu, Mengru Mira Zhang, Michael L. Gross · 2020 · Chemical Reviews
  64. Activity-based probes for proteomic profiling of histone deacetylase complexes
    Cleo M. Salisbury, Benjamin F. Cravatt · 2007 · Proceedings of the National Academy of Sciences
  65. Challenges and Prospects of Plant Proteomics
    Klaas Jan van Wijk · 2001 · PLANT PHYSIOLOGY
  66. Automatic Poisson peak harvesting for high throughput protein identification
    Edmond J. Breen, Femia G. Hopwood, Keith L. Williams, Marc Ronald Wilkins · 2000 · Electrophoresis
  67. Flow induces epithelial-mesenchymal transition, cellular heterogeneity and biomarker modulation in 3D ovarian cancer nodules
    Rizvi, Imran, Gurkan, Umut A., Tasoglu, Savas, Alagic, Nermina, Celli, Jonathan P., Mensah, Lawrence B., Mai, Zhiming, Demirci, Utkan, Hasan, Tayyaba · 2013 · Proceedings of the National Academy of Sciences
  68. Extracellular Redox State: Refining the Definition of Oxidative Stress in Aging
    Dean P. Jones · 2006 · Rejuvenation Research
  69. A streamlined platform for high-content functional proteomics of primary human specimens
    Nadim Jessani, Sherry Niessen, Binqing Q. Wei, Monica M Nicolau, Mark Humphrey, Youngran Ji, Wonshik Han, Dong‐Young Noh, John R. Yates, Stefanie S. Jeffrey et al. · 2005 · Nature Methods
  70. The speciation of the proteome
    Peter Roman Jungblut, Hermann−Georg Holzhütter, Rolf Apweiler · 2008 · Chemistry Central Journal
  71. Proteomics Technologies and Challenges
    William Chi Shing Cho · 2007 · Genomics Proteomics & Bioinformatics
  72. Robust and Sensitive iTRAQ Quantification on an LTQ Orbitrap Mass Spectrometer
    Marcus Bantscheff, Markus Böesche, Dirk Eberhard, Toby Mathieson, Gavain M. A. Sweetman, Bernhard Küster · 2008 · Molecular & Cellular Proteomics
  73. Chemical proteomics and its application to drug discovery
    Douglas A. Jeffery, Matthew S. Bogyo · 2003 · Current Opinion in Biotechnology
  74. Applying Mechanisms of Chemical Toxicity to Predict Drug Safety
    Frederick Peter Guengerich, James S. MacDonald · 2007 · Chemical Research in Toxicology
  75. De novo peptide sequencing and quantitative profiling of complex protein mixtures using mass-coded abundance tagging
    Gerard Cagney, Andrew Emili · 2002 · Nature Biotechnology
  76. Protein Identification by Mass Spectrometry
    M. A. Baldwin · 2003 · Molecular & Cellular Proteomics
  77. Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy
    Pyong‐Gon Moon, Jeong‐Eun Lee, Sungyong You, Taek‐Kyun Kim, Ji‐Hoon Cho, In‐San Kim, Tae‐Hwan Kwon, Chan‐Duck Kim, Sun-Hee Park, Daehee Hwang et al. · 2011 · PROTEOMICS
  78. Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry–Based Assays
    Andrew N. Hoofnagle, Jeffrey R. Whiteaker, Steven A. Carr, Eric W. Kuhn, Tao Liu, Sam A. Massoni, Stefani N. Thomas, R. Reid Townsend, Lisa J. Zimmerman, Emily S. Boja et al. · 2015 · Clinical Chemistry
  79. Protein Identification by Tandem Mass Spectrometry and Sequence Database Searching
    Alexey I. Nesvizhskii · 2006 · Humana Press eBooks
  80. Comparative Functional Analysis of the Caenorhabditis elegans and Drosophila melanogaster Proteomes
    Schrimpf, Sabine P, Weiss, Manuel, Reiter, Lukas, Ahrens, Christian H, Jovanovic, Marko, Malmström, Johan, Brunner, Erich, Mohanty, Sonali, Lercher, Martin J, Hunziker, Peter E et al. · 2009 · PLoS Biology
  81. Profiling Core Proteomes of Human Cell Lines by One-dimensional PAGE and Liquid Chromatography-Tandem Mass Spectrometry
    Markus Schirle, Marie-Anne Heurtier, Bernhard Küster · 2003 · Molecular & Cellular Proteomics
  82. Proteomic Identification of a Stress Protein, Mortalin/mthsp70/GRP75
    Jinghua Jin, Christine Hulette, Yan Wang, Terry Zhang, Catherine Pan, Renu Wadhwa, Jing Zhang · 2006 · Molecular & Cellular Proteomics
  83. Phospho.ELM: a database of phosphorylation sites update 2008
    Francesca Diella, C. M. Gould, Claudia Chica, Allegra Via, Toby J. Gibson · 2007 · Nucleic Acids Research
  84. Chromatographic Isolation of Methionine-containing Peptides for Gel-free Proteome Analysis
    Kris Gevaert, Jo Van Damme, Marc Goethals · 2002 · Molecular & Cellular Proteomics
  85. Sources of Technical Variability in Quantitative LC–MS Proteomics: Human Brain Tissue Sample Analysis
    Paul Piehowski, Vladislav Petyuk, Danny Orton, Fang Xie, Ronald Moore, Manuel Ramirez‐Restrepo, Anzhelika Engel, Andrew P. Lieberman, Roger L. Albin, David Camp et al. · 2013 · Journal of Proteome Research
  86. Stable-Isotope Dilution LC–MS for Quantitative Biomarker Analysis
    Eugene Ciccimaro, Ian A. Blair · 2010 · Bioanalysis
  87. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in clinical chemistry
    Laure Marvin, Matthew Roberts, Laurent Bernard Fay · 2003 · Clinica Chimica Acta
  88. Tools for Label-free Peptide Quantification
    Sven Nahnsen, Chris Bielow, Knut Reinert, Oliver Kohlbacher · 2012 · Molecular & Cellular Proteomics
  89. Shotgun proteomics: tools for the analysis of complex biological systems.
    Christine C. Wu, Michael J. MacCoss · 2002 · PubMed
  90. Genomic and proteomic expression analysis of Leishmania promastigote and amastigote life stages: The Leishmania genome is constitutively expressed
    Kirk Ryan. Leifso, Gabriela Cohen‐Freue, Nisha Dogra · 2006 · Molecular and Biochemical Parasitology
  91. Proteomic technologies for the identification of disease biomarkers in serum: Advances and challenges ahead
    Sandipan Ray, Panga Jaipal Reddy, Rekha Jain, Kishore Gollapalli, Aliasgar V. Moiyadi, Sanjeeva Srivastava · 2011 · PROTEOMICS
  92. Updated Biological Roles for Matrix Metalloproteinases and New “Intracellular” Substrates Revealed by Degradomics
    Georgina S. Butler, Christopher M. Overall · 2009 · Biochemistry
  93. Liquid chromatography mass spectrometry-based proteomics: Biological and technological aspects
    Yuliya V. Karpievitch, Ashoka D. Polpitiya, Gordon Anderson, Richard D. Smith, Alan R. Dabney · 2010 · The Annals of Applied Statistics
  94. Limitations of current proteomics technologies
    Spiros D. Garbis, Gert Lübec, Michael Fountoulakis · 2005 · Journal of Chromatography A
  95. Triplex protein quantification based on stable isotope labeling by peptide dimethylation applied to cell and tissue lysates
    Boersema, Paul J., Aye, Thin Thin, van Veen, Toon A. B., Heck, Albert J. R., Mohammed, Shabaz · 2008 · PROTEOMICS
  96. Degradomics: Systems biology of the protease web. Pleiotropic roles of MMPs in cancer
    Christopher M. Overall, Richard A. Dean · 2006 · Cancer and Metastasis Reviews
  97. Global organellar proteomics
    Steven W. Taylor, Eoin Fahy, Soumitra S. Ghosh · 2003 · Trends in biotechnology
  98. Protein Profiling with Cleavable Isotope-coded Affinity Tag (cICAT) Reagents
    Jiaxu Li, Hanno Steen, Steven P. Gygi · 2003 · Molecular & Cellular Proteomics
  99. The development and application of methods for activity-based protein profiling
    Nadim Jessani, Benjamin F. Cravatt · 2003 · Current Opinion in Chemical Biology
  100. Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics
    Fang Xie, Tao Liu, Weijun Qian, Vladislav Petyuk, Richard D. Smith · 2011 · Journal of Biological Chemistry