Insulin stimulates myogenesis in a rat myoblast line

MANDEL, JEAN-LOUIS; PEARSON, MARK L.

doi:10.1038/251618a0

Cited by 86Muscle Physiology and DisordersMetabolism, Diabetes, and CancerMuscle metabolism and nutrition

Insulin stimulates myogenesis in a rat myoblast line

MANDEL, JEAN-LOUIS, PEARSON, MARK L.·1974·Nature

DOI 10.1038/251618a0

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After 7-9 days of culture, L6 myoblasts had fused into multinucleated myotubes.
“After 7–9 days of culture the myoblasts had fused into multinucleated myotubes 41.”
From Akt-dependent anabolic activity of natural and synthetic brassinosteroids in rat skeletal muscle cells (2011) · cites this paper as [41]

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“2% dextrancoated charcoal-treated FBS and 10 –6 M insulin (18).”
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The Development of Insulin Receptors and Responsiveness Is an Early Marker of Differentiation in the Muscle Cell Line L6*
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Autonomous differentiation in the mouse myogenic cell line, C2, involves a mutual positive control between insulin-like growth factor II and MyoD, operating as early as at the myoblast stage
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PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Roles for insulin-supported skeletal muscle growth1,2
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“The physiological effects of insulin have been studied in satellite cells of rodents (de la Haba et al., 1966; Mandel and Pearson, 1974; Allen et al., 1985; Illario et al., 2009), cattle (Cassar-Malek et al., 1999), and fish (Castillo et al., 2004).”
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“After 7–9 d of culture, the myoblasts had fused into multinucleated myotubes (31).”
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Serum-Free Culture Conditions for Analysis of Secretory Proteinases during Myogenic Differentiation of Mouse C2C12 Myoblasts
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Testosterone Increases the Expression and Phosphorylation of AMP Kinase α in Men With Hypogonadism and Type 2 Diabetes
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CYCLIC NUCLEOTIDES
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“L6 cells are originally derived from rat skeletal muscle, and propagate as mononucleated myoblasts, but differentiate into multinucleated myotubes upon incubation in low-serumcontaining medium (Yaffe, 1968; Richler and Yaffe, 1970; Mandel and Pearson, 1974; Yaffe and Saxel, 1977).”
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Growth and differentiation of chicken embryo muscle cell cultures derived from fast- and slow-growing lines
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“Insulin is known to regulate both cell growth and differentiation in many cell types, including muscle (34), mammary gland (35), adipose (36) and others.”
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Induction of myogenic differentiation in serum-free medium does not require DNA synthesis
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Control of differentiation in heterokaryons and hybrids involving differentiation-defective myoblast variants.
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“Rat L6 skeletal muscle cells (Institute of Materia Medica, Shanghai, China) were grown in high glucose DMEM containing 4500 mg/L D-glucose, 10% fetal bovine serum (FBS), 100 U/ml penicillin, 100 mg/ml streptomycin (Invitrogen, Grand Island, NY, USA) until 40% confluent and then altered with differentiating media (2% F…”
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Myogenic Differentiation of L6 Rat Myoblasts: Evidence for Pleiotropic Effects on Myogenesis by RNA Polymerase II Mutations to α-Amanitin Resistance
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“Clones containing a chromosome number appropriate for hybrids between the parental cells were then replated at clonal density and allowed to grow for 2 wk to form large colonies, then stimulated to differentiate by feeding with medium containing I% fetal bovine serum and 5 ~g/ml insulin (12).”
The INSR/AKT/mTOR pathway regulates the pace of myogenesis in a syndecan-3-dependent manner
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Huang, F. L., & Tao, S.-H. (1980) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(80)90264-7 · Cited by 6 · see citing works
Long-term treatment with insulin and retinoic acid increased glucose utilization in L6 muscle cells via glycogenesis
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“Insulin has been shown to enhance fusion of myoblast into myotubes and activity of creatine kinase in L6 cells (Mandel and Pearson 1974).”
Background
M-Type 6-Phosphofructo-2-kinase/Fructose-2,6-bisphosphatase Is the Product of a Late Muscle Differentiation Gene
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“…derived from rat skeletal muscle, propagate as mononucleated myoblasts, and differentiate into multinucleated myotubes upon incubation in low-serumcontaining medium (Mandel & Pearson, 1974; Richler & Yaffe, 1970; Yaffe, 1968; Yaffe & Saxel, 1977a, b) but do not form actomyosin cross-striations.”
Background
SDC3 acts as a timekeeper of myogenic differentiation by regulating the insulin/AKT/mTOR axis in muscle stem cell progenyPreprint
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“It appears to promote 68 both myoblast proliferation and differentiation (Conejo et al., 2001; Grabiec et al., 2014; Grzelkowska69 Kowalczyk et al., 2013; Mandel and Pearson, 1974).”
Background
Proliferation of Myocytes on Different ECM-Components of Animal/Human Origin in Vitro
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“thigh muscle origin (5) were used in proliferation assays to evaluate the different extracellular matrices.”
Method
Experimental Validation of an In Vitro Method for Assessing Insulin-Dependent Glucose Uptake
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“CRL-1458) (Mandel and Pearson, 1974) were obtained from ATCC (Manassas, VA) and were grown using Dulbecco's Modified Eagle Medium (DMEM, Gibco-BRL) supplemented with 4 mM L-glutamine, 1.0 mM sodium pyruvate, 4.5 g/L glucose, 1.5 g/L sodium bicarbonate, 10 % heat-inactivated fetal calf serum (FCS),…”
MethodInfluential
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Self-citation
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Identification of Flavonoids-based HITS to Inhibit GSK3β Activity: An In Silico and In Vitro Analysis
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The Development of Insulin Receptors and Responsiveness Is an Early Marker of Differentiation in the Muscle Cell Line L6*
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Authors: MANDEL, J.-L., & PEARSON, M. L.
Affiliations: University of Toronto (CA)
ORCID: MANDEL, JEAN-LOUIS
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 1974-10
Volume: 251
Issue: 5476
Pages: 618-620
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/251618a0
OpenAlex: W1994361005
Cited by: 84
References: 17
Citations by year: 2026: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2020: 2 · 2019: 2 · 2016: 2 · 2015: 1 · 2014: 1
FWCI: 2.09
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Keywords: insulin, myogenesis, L6 myoblasts, myoblast fusion, multinucleated myotubes, cyclic AMP, adenylate cyclase, creatine kinase, myosin, serum requirement
MeSH terms: Animals, Cell Differentiation, Cell Fusion, Cell Line, Creatine Kinase, Insulin, Morphogenesis, Muscles, Stimulation, Chemical, Rats

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