Reverse gyrase—a topoisomerase which introduces positive superhelical turns into DNA
Abstract
An enzymatic activity which converts closed circular DNA into a positively supercoiled form is present in cellular extracts of Sulfolobus, an acidothermophilic archaebacterium. This novel enzyme is a type II DNA topoisomerase and is active only at temperatures greater than 55 degrees C, in the presence of ATP and Mg2+.
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Reverse gyrase is critical to hyperthermophilic life.
“Reverse gyrase is critical to hyperthermophilic life (Forterre 1996;Forterre 2002;Kikuchi and Asai 1984;Heine and Chandra 2009).”
From Environmental Adaptation from the Origin of Life to the Last Universal Common Ancestor (2018) · cites this paper as Kikuchi and Asai 1984
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Brochier-Armanet, C., & Forterre, P. (2007) · Archaea · doi:10.1155/2006/582916 · Cited by 97 · see citing works
Environmental Adaptation from the Origin of Life to the Last Universal Common Ancestor
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Forterre, P., Confalonier, F., Charbonnier, F., & Duguet, M. (1995) · Origins of Life and Evolution of the Biosphere · doi:10.1007/bf01581587 · Cited by 52 · see citing works
- Authors
- Kikuchi, A., & Asai, K.
- ORCID
- Kikuchi, Akihiko
- Venue
- Nature
- Published
- 1984-06
- Volume
- 309
- Issue
- 5970
- Pages
- 677-681
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2074194645
- Cited by
- 292
- References
- 31
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 10 · 2023: 8 · 2022: 3 · 2021: 5 · 2020: 8 · 2019: 7 · 2018: 5 · 2017: 6 · 2016: 2 · 2015: 3 · 2014: 10 · 2013: 9 · 2012: 3
- FWCI
- 4.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer therapeutics and mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- reverse gyrase, topoisomerase II, positive supercoiling, DNA superhelix, Sulfolobus, high-temperature enzyme activity, ATP-dependent enzyme, magnesium-dependent enzyme
- MeSH terms
- DNA Topoisomerases, Type II, DNA Topoisomerases, Type I, DNA, Superhelical, Gram-Negative Bacteria, Hot Temperature, Kinetics, Plasmids
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