Cited by 292Cancer therapeutics and mechanismsBacterial Genetics and BiotechnologyDNA and Nucleic Acid Chemistry

Reverse gyrase—a topoisomerase which introduces positive superhelical turns into DNA

Kikuchi, Akihiko, Asai, Keiko·1984·Nature

Abstract

An enzymatic activity which converts closed circular DNA into a positively supercoiled form is present in cellular extracts of Sulfolobus, an acidothermophilic archaebacterium. This novel enzyme is a type II DNA topoisomerase and is active only at temperatures greater than 55 degrees C, in the presence of ATP and Mg2+.

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Crossref · 10.1038/309677a0

Cites

31 of 31 references verified0 citation errors · 0 not checkable

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Cited by

Authors
Kikuchi, A., & Asai, K.
Venue
Nature
Published
1984-06
Volume
309
Issue
5970
Pages
677-681
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2074194645
Cited by
292
References
31
Citations by year
2026: 6 · 2025: 11 · 2024: 10 · 2023: 8 · 2022: 3 · 2021: 5 · 2020: 8 · 2019: 7 · 2018: 5 · 2017: 6 · 2016: 2 · 2015: 3 · 2014: 10 · 2013: 9 · 2012: 3
FWCI
4.25
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer therapeutics and mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
reverse gyrase, topoisomerase II, positive supercoiling, DNA superhelix, Sulfolobus, high-temperature enzyme activity, ATP-dependent enzyme, magnesium-dependent enzyme
MeSH terms
DNA Topoisomerases, Type II, DNA Topoisomerases, Type I, DNA, Superhelical, Gram-Negative Bacteria, Hot Temperature, Kinetics, Plasmids

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