Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor
Abstract
The sequence of 5,037 amino acids composing the ryanodine receptor from rabbit skeletal muscle sarcoplasmic reticulum has been deduced by cloning and sequencing the complementary DNA. The predicted structure suggests that the calcium release channel activity resides in the C-terminal region of the receptor molecule, whereas the remaining portion constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum and the transverse tubule.
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RyR proteins contain a markedly greater number of sulfhydryl groups compared to other contractile proteins, which supports the proposed hypothesis that NO-mediated RyR modulation and Ca2+ release could contribute to enhanced muscle contractility following NO3− supplementation.
“In addition, RyR proteins contain a markedly greater number of sulfhydryl groups compared to other contractile proteins [146], which supports the proposed hypothesis that NO-mediated RyR modulation and Ca2+ release could contribute to enhanced muscle contractility following NO3− supplementation [123].”
From Effects of Dietary Nitrate Supplementation on Weightlifting Exercise Performance in Healthy Adults: A Systematic Review (2020) · cites this paper as [146]
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- Authors
- Takeshima, H., Nishimura, S., Matsumoto, T., Ishida, H., Kangawa, K., Minamino, N., Matsuo, H., Ueda, M., Hanaoka, M., Hirose, T., & Numa, S.
- Affiliations
- Kyoto University (JP)
- University of Miyazaki (JP)
- Keio University (JP)
- Venue
- Nature
- Published
- 1989-06
- Volume
- 339
- Issue
- 6224
- Pages
- 439-445
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2039547987
- Cited by
- 1,146
- References
- 51
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 2 · 2023: 10 · 2022: 10 · 2021: 12 · 2020: 16 · 2019: 15 · 2018: 17 · 2017: 19 · 2016: 18 · 2015: 13 · 2014: 24 · 2013: 16 · 2012: 26
- FWCI
- 28.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- ryanodine receptor, skeletal muscle, sarcoplasmic reticulum, calcium release channel, cDNA, amino acid sequence, T-tubules
- MeSH terms
- Amino Acid Sequence, Animals, Base Sequence, Calcium Channel Blockers, Cloning, Molecular, DNA, Molecular Sequence Data, Muscles, Protein Conformation, Rabbits, Receptors, Cholinergic, RNA, Messenger, Sarcoplasmic Reticulum, Transcription, Genetic, Restriction Mapping, Ryanodine Receptor Calcium Release Channel
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