Cited by 1,146Ion channel regulation and functionCardiac electrophysiology and arrhythmiasMuscle Physiology and Disorders

Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor

Takeshima, Hiroshi, Nishimura, Seiichiro, Matsumoto, Takeshi, Ishida, Hiroyuki, Kangawa, Kenji, Minamino, Naoto, Matsuo, Hisayuki, Ueda, Masamichi +3 more·1989·Nature

Abstract

The sequence of 5,037 amino acids composing the ryanodine receptor from rabbit skeletal muscle sarcoplasmic reticulum has been deduced by cloning and sequencing the complementary DNA. The predicted structure suggests that the calcium release channel activity resides in the C-terminal region of the receptor molecule, whereas the remaining portion constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum and the transverse tubule.

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Crossref · 10.1038/339439a0

Cites

51 of 51 references verified0 citation errors · 0 not checkable

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  • RyR proteins contain a markedly greater number of sulfhydryl groups compared to other contractile proteins, which supports the proposed hypothesis that NO-mediated RyR modulation and Ca2+ release could contribute to enhanced muscle contractility following NO3− supplementation.

    “In addition, RyR proteins contain a markedly greater number of sulfhydryl groups compared to other contractile proteins [146], which supports the proposed hypothesis that NO-mediated RyR modulation and Ca2+ release could contribute to enhanced muscle contractility following NO3− supplementation [123].”

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Authors
Takeshima, H., Nishimura, S., Matsumoto, T., Ishida, H., Kangawa, K., Minamino, N., Matsuo, H., Ueda, M., Hanaoka, M., Hirose, T., & Numa, S.
Affiliations
  • Kyoto University (JP)
  • University of Miyazaki (JP)
  • Keio University (JP)
Venue
Nature
Published
1989-06
Volume
339
Issue
6224
Pages
439-445
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2039547987
Cited by
1,146
References
51
Citations by year
2026: 3 · 2025: 8 · 2024: 2 · 2023: 10 · 2022: 10 · 2021: 12 · 2020: 16 · 2019: 15 · 2018: 17 · 2017: 19 · 2016: 18 · 2015: 13 · 2014: 24 · 2013: 16 · 2012: 26
FWCI
28.84
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
ryanodine receptor, skeletal muscle, sarcoplasmic reticulum, calcium release channel, cDNA, amino acid sequence, T-tubules
MeSH terms
Amino Acid Sequence, Animals, Base Sequence, Calcium Channel Blockers, Cloning, Molecular, DNA, Molecular Sequence Data, Muscles, Protein Conformation, Rabbits, Receptors, Cholinergic, RNA, Messenger, Sarcoplasmic Reticulum, Transcription, Genetic, Restriction Mapping, Ryanodine Receptor Calcium Release Channel

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