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Cited by 456

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Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin

Open access
Tada, Shusuke, Li, Anatoliy, Maiorano, Domenico, Méchali, Marcel, Blow, J. Julian·2001·Nature Cell Biology
DOI 10.1038/35055000
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Abstract

Eukaryotic replication origins are 'licensed' for replication early in the cell cycle by loading Mcm(2–7) proteins. As chromatin replicates, Mcm(2–7) are removed, thus preventing the origin from firing again. Here we report the purification of the RLF-B component of the licensing system and show that it corresponds to Cdt1. RLF-B/Cdt1 was inhibited by geminin, a protein that is degraded during late mitosis. Immunodepletion of geminin from metaphase extracts allowed them to assemble licensed replication origins. Inhibition of CDKs in metaphase stimulated origin assembly only after the depletion of geminin. These experiments suggest that geminin-mediated inhibition of RLF-B/Cdt1 is essential for repressing origin assembly late in the cell cycle of higher eukaryotes.

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Metadata

Authors
Tada, S., Li, A., Maiorano, D., Méchali, M., & Blow, J. J.
Venue
Nature Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2001-02
Volume
3
Issue
2
Pages
107-113
ISSN
  • 1465-7392 (ISSN-L)
  • 1476-4679
DOI
10.1038/35055000
OpenAlex
W2128056082
Cited by
456
References
0
FWCI
17.26
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin. https://app.cochat.ai/papers/10.1038/35055000
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Tada, S., Li, A., Maiorano, D., Méchali, M., & Blow, J. J. (2001). Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin. Nature Cell Biology. https://doi.org/10.1038/35055000

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