Regulation of transport of the dopamine D1 receptor by a new membrane-associated ER protein
Abstract
Many structural determinants for G protein-coupled receptor (GPCR) functions have been defined, but little is known concerning the regulation of their transport from the endoplasmic reticulum (ER) to the cell surface. Here we show that a carboxy-terminal hydrophobic motif, FxxxFxxxF, which is highly conserved among GPCRs, functions independently as an ER-export signal for the dopamine D1 receptor. A newly identified ER-membrane-associated protein, DRiP78, binds to this motif. Overexpression or sequestration of DRiP78 leads to retention of D1 receptors in the ER, reduced ligand binding, and a slowdown in the kinetics of receptor glycosylation. Our results indicate that DRiP78 may regulate the transport of a GPCR by binding to a specific ER-export signal.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A DRIP78 binding sequence (FxxxFxxxF) is conserved in the C-termini of a subset of group B and group C surreal-GPCR sequences.
“In line with such a role, we find a dopamine receptor interacting protein 78 (DRIP78) binding sequence (FxxxFxxxF) (Bermak et al., 2001) conserved in the C-termini of a subset of sequences ( SPU_006834, SPU_008884, SPU_010318, SPU_010318; SPU_000576, SPU_015119, SPU_006431).”
From Opsins and clusters of sensory G-protein-coupled receptors in the sea urchin genome (2006) · cites this paper as Bermak et al., 2001
Cites
Antibodies, a laboratory manual
(1989) · Biomedicine & Pharmacotherapy · doi:10.1016/0753-3322(89)90130-3 · Cited by 12,318 · see citing works
Crystal Structure of Rhodopsin: A G Protein-Coupled Receptor
Palczewski, K., Kumasaka, T., Hori, T., Behnke, C. A., Motoshima, H., Fox, B. A., Trong, I. L., Teller, D. C., Okada, T., Stenkamp, R. E., et al. (2000) · Science · doi:10.1126/science.289.5480.739 · Cited by 5,637 · see citing works
Antibodies: A laboratory manual
J.L. (1989) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(89)90307-1 · Cited by 5,141 · see citing works
Prediction of protein conformation
Chou, P. Y., & Fasman, G. D. (1974) · Biochemistry · doi:10.1021/bi00699a002 · Cited by 3,309 · see citing works
RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor
McLatchie, L. M., Fraser, N. J., Main, M. J., Wise, A., Brown, J., Thompson, N., Solari, R., Lee, M. G., & Foord, S. M. (1998) · Nature · doi:10.1038/30666 · Cited by 2,134 · see citing works
MODEL SYSTEMS FOR THE STUDY OF SEVEN-TRANSMEMBRANE-SEGMENT RECEPTORS
Dohlman, H. G., Thorner, J. W., Caron, M. G., & Lefkowitz, R. J. (1991) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.60.070191.003253 · Cited by 1,377 · see citing works
Ji, T. H., Grossmann, M., & Ji, I. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.28.17299 · Cited by 1,291 · see citing works
Protein Sorting by Transport Vesicles
Rothman, J. E., & Wieland, F. (1996) · Science · doi:10.1126/science.272.5259.227 · Cited by 1,156 · see citing works
GABAB receptors function as a heteromeric assembly of the subunits GABABR1 and GABABR2
JONES, K. A., Borowsky, B., Tamm, J. A., Craig, D. A., Durkin, M. M., Dai, M., Yao, W.-J., Johnson, M., Gunwaldsen, C., Huang, L., et al. (1998) · Nature · doi:10.1038/25348 · Cited by 1,081 · see citing works
Sequence Alignment of the G-Protein Coupled Receptor Superfamily
PROBST, W. C., Snyder, L., Schuster, D. I., Brosius, J., & Sealfon, S. C. (1992) · DNA and Cell Biology · doi:10.1089/dna.1992.11.1 · Cited by 841 · see citing works
Cloning and expression of human and rat Dt dopamine receptors
Zhou, Q., Grandy, D. K., Thambi, L., Kushner, J. A., Tol, H. H. M. V., Cone, R. D., Pribnow, D. G., Salon, J. A., Bunzow, J. R., & Civelli, O. (1990) · Nature · doi:10.1038/347076a0 · Cited by 562 · see citing works
Self-citationRegulatory mechanisms that modulate signalling by G-protein-coupled receptors
BÖHM, S. K., Grady, E. F., & Bunnett, N. W. (1997) · Biochemical Journal · doi:10.1042/bj3220001 · Cited by 508 · see citing works
A Di-Acidic Signal Required for Selective Export from the Endoplasmic Reticulum
Nishimura, N., & Balch, W. E. (1997) · Science · doi:10.1126/science.277.5325.556 · Cited by 488 · see citing works
Tai, A. W., Chuang, J.-Z., Bode, C., Uwe Wolfrum, B., & Sung, C.-H. (1999) · Cell · doi:10.1016/s0092-8674(00)80800-4 · Cited by 478 · see citing works
Balch, W. E., McCaffery, J. M., Plutner, H., & Farquhar, M. G. (1994) · Cell · doi:10.1016/0092-8674(94)90359-x · Cited by 389 · see citing works
Regulation of MHC Class I Transport by the Molecular Chaperone, Calnexin (p88, IP90)
Jackson, M. R., Cohen-Doyle, M. F., Peterson, P. A., & Williams, D. B. (1994) · Science · doi:10.1126/science.8278813 · Cited by 355 · see citing works
gp25L/emp24/p24 Protein Family Members of the cis- Golgi Network Bind Both COP I and II Coatomer
Dominguez, M., Dejgaard, K., Füllekrug, J., Dahan, S., Fazel, A., Paccaud, J., Thomas, D. Y., Bergeron, J. J. M., & Nilsson, T. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.4.751 · Cited by 335 · see citing works
Bimodal Interaction of Coatomer with the p24 Family of Putative Cargo Receptors
Fiedler, K., Veit, M., Stamnes, M. A., & Rothman, J. E. (1996) · Science · doi:10.1126/science.273.5280.1396 · Cited by 325 · see citing works
The cyclophilin homolog ninaA is required in the secretory pathway
Colley, N. J., Baker, E. K., Stamnes, M. A., & Zuker, C. S. (1991) · Cell · doi:10.1016/0092-8674(91)90177-z · Cited by 324 · see citing works
Petäjä‐Repo, U. E., Hogue, M., Laperrière, A., Walker, P., & Bouvier, M. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.18.13727 · Cited by 290 · see citing works
The Recycling of ERGIC-53 in the Early Secretory Pathway
Kappeler, F., Klopfenstein, D. R., Foguet, M., Paccaud, J., & Hauri, H. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.50.31801 · Cited by 266 · see citing works
Dwyer, N. D., Troemel, E. R., Sengupta, P., & Bargmann, C. I. (1998) · Cell · doi:10.1016/s0092-8674(00)81173-3 · Cited by 249 · see citing works
Barak, L. S., Ferguson, S. S. G., Zhang, J., Martenson, C. H., Meyer, T., & Caron, M. G. (1997) · Molecular Pharmacology · doi:10.1124/mol.51.2.177 · Cited by 219 · see citing works
Cyclophilin-related protein RanBP2 acts as chaperone for red/green opsin
Ferreira, P. A., Nakayama, T. A., Pak, W. L., & Travis, G. H. (1996) · Nature · doi:10.1038/383637a0 · Cited by 216 · see citing works
Defects in G Protein-Coupled Signal Transduction in Human Disease
Spiegel, A. M. (1996) · Annual Review of Physiology · doi:10.1146/annurev.ph.58.030196.001043 · Cited by 195 · see citing works
Tarasova, N. I., Stauber, R. H., Choi, J. K., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.23.14817 · Cited by 177 · see citing works
The general concept of molecular chaperones
Ellis, R. J. (1993) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.1993.0023 · Cited by 152 · see citing works
Folding of Secretory and Membrane Proteins
Kuznetsov, G., & Nigám, S. K. (1998) · New England Journal of Medicine · doi:10.1056/nejm199812033392307 · Cited by 139 · see citing works
Modulation of Dopamine D2 Receptor Signaling by Actin-Binding Protein (ABP-280)
Li, M., Bermak, J. C., Wang, Z. W., & Zhou, Q. Y. (2000) · Molecular Pharmacology · doi:10.1124/mol.57.3.446 · Cited by 136 · see citing works
Self-citationMultiple GTP-binding proteins regulate vesicular transport from the ER to Golgi membranes.
Schwaninger, R., Plutner, H., Bokoch, G., & Balch, W. E. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.119.5.1077 · Cited by 123 · see citing works
Mizuno, M., & Singer, S. J. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.12.5732 · Cited by 121 · see citing works
Rodríguez, M. C., Xie, Y., Wang, H., Collison, K. S., & Segaloff, D. L. (1992) · Molecular Endocrinology · doi:10.1210/mend.6.3.1316539 · Cited by 100 · see citing works
Three-Dimensional Structure of the Cytoplasmic Face of the G Protein Receptor Rhodopsin
Yèagle, P. L., Alderfer, J. L., & Albert, A. D. (1997) · Biochemistry · doi:10.1021/bi970908a · Cited by 81 · see citing works
Locker, J. K., Griffiths, G. W., Horzinek, M. C., et al. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)49683-x · Cited by 77 · see citing works
Oksche, A., Dehe, M., Schülein, R., Wiesner, B., & Rosenthal, W. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)00140-9 · Cited by 69 · see citing works
Sorting Determinants in the Transmembrane Domain of p24 Proteins
Fiedler, K., & Rothman, J. E. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.40.24739 · Cited by 52 · see citing works
Pascale, M. C., Erra, M. C., Malagolini, N., et al. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)74024-1 · Cited by 40 · see citing works
Hemochromatosis: a genetic defect in iron metabolism
Jazwinska, E. C. (1998) · BioEssays · doi:10.1002/(sici)1521-1878(199807)20:7 · Cited by 15 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Lin, D. C.-H., Bullock, C. M., Ehlert, F. J., Chen, J.-L., Tian, H., & Zhou, Q.-Y. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202139200 · Cited by 313 · see citing works
Self-citationOpsins and clusters of sensory G-protein-coupled receptors in the sea urchin genome
Raible, F., Tessmar-Raible, K., Arboleda, E., Kaller, T., Bork, P., Arendt, D., & Arnone, M. I. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.08.070 · Cited by 170 · see citing works
Noon, L., Franklin, J., King, P., Goulding, N., Hunyady, L., & Clark, A. (2002) · Journal of Endocrinology · doi:10.1677/joe.0.1740017 · Cited by 88 · see citing works
The melanocortin receptors and their accessory proteins
Ramachandrappa, S., Gorrigan, R. J., Clark, A. J. L., & Chan, L. F. (2013) · Frontiers in Endocrinology · doi:10.3389/fendo.2013.00009 · Cited by 87 · see citing works
The human intronless melanocortin 4-receptor gene is NMD insensitive
Brocke, K. S. (2002) · Human Molecular Genetics · doi:10.1093/hmg/11.3.331 · Cited by 84 · see citing works
Cooray, S. N., Almiro Do Vale, I., Leung, K.-Y., Webb, T. R., Chapple, J. P., Egertová, M., Cheetham, M. E., Elphick, M. R., & Clark, A. J. L. (2008) · Endocrinology · doi:10.1210/en.2007-1463 · Cited by 73 · see citing works
- Authors
- Bermak, J. C., Li, M., Bullock, C., & Zhou, Q.-Y.
- Affiliations
- University of California, Irvine (US)
- Corresponding author
- Zhou, Qun-Yong
- ORCID
- Li, Ming
- Venue
- Nature Cell Biology
- Published
- 2001-04-17
- Volume
- 3
- Issue
- 5
- Pages
- 492-498
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W1527823690
- Cited by
- 281
- References
- 38
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 1 · 2023: 7 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 6 · 2018: 4 · 2017: 8 · 2016: 12 · 2015: 10 · 2014: 7 · 2013: 14 · 2012: 20
- FWCI
- 5.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Alfred P. Sloan Foundation
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Endoplasmic Reticulum Stress and Disease
- Keywords
- dopamine D1 receptor, G protein-coupled receptors, receptor transport, receptor glycosylation, ligand binding, receptor trafficking
- MeSH terms
- Cyclic AMP, Amino Acid Sequence, Animals, Biological Transport, Cell Line, Cell Membrane, Endoplasmic Reticulum, Humans, Kinetics, Ligands, Luminescent Proteins, Microscopy, Fluorescence, Models, Biological, Molecular Sequence Data, Receptors, Cell Surface, Recombinant Fusion Proteins, Time Factors, Mutagenesis, Site-Directed, CD8 Antigens, Sequence Homology, Amino Acid, Protein Structure, Tertiary, Receptors, Dopamine D1, Microscopy, Confocal, Two-Hybrid System Techniques, Amino Acid Motifs, Green Fluorescent Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review