Cited by 433Enzyme Structure and FunctionDNA Repair MechanismsProtein Structure and Dynamics

Crystal structure of a DExx box DNA helicase

Subramanya, Hosahalli S., Bird, Louise E., Brannigan, James A., Wigley, Dale B.·1996·Nature

Abstract

There are a wide variety of helicases that unwind helical DNA and RNA substrates. The twelve helicases that have been identified in Escherichia coli play a role in almost all cellular processes involving nucleic acids. We have solved the crystal structure of a monomeric form of a DNA helicase from Bacillus stearothermophilus, alone and in a complex with ADP, at 2.5 and 2.9 A resolution, respectively. The enzyme comprises two domains with a deep cleft running between them. The ATP-binding site, which is situated at the bottom of this cleft, is formed by motifs that are conserved across the superfamily of related helicases. Unexpected structural homology with the DNA recombination protein, RecA, suggests how ATP binding and hydrolysis may drive conformational changes of the enzyme during catalysis, and implies that there is a common mechanism for all helicases.

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Crossref · 10.1038/384379a0

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29 of 29 references verified0 citation errors · 0 not checkable

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Research works printed but not linked: 1

  1. Opperman, T. & Richardson, J. P. J. Bacteriol. 176, 6033–5043 (1994).

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Authors
Subramanya, H. S., Bird, L. E., Brannigan, J. A., & Wigley, D. B.
Affiliations
University of Oxford (GB)
Venue
Nature
Published
1996-11
Volume
384
Issue
6607
Pages
379-383
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2026133647
Cited by
433
References
29
Citations by year
2026: 7 · 2025: 5 · 2024: 2 · 2023: 5 · 2022: 2 · 2021: 8 · 2020: 4 · 2019: 3 · 2018: 3 · 2017: 3 · 2016: 6 · 2015: 5 · 2014: 12 · 2013: 19 · 2012: 12
FWCI
32.76
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
DNA helicase, Bacillus stearothermophilus, crystal structure, ADP complex, ATP binding site, ATP hydrolysis, conformational change
MeSH terms
Adenosine Diphosphate, Amino Acid Sequence, Geobacillus stearothermophilus, DNA Helicases, Models, Molecular, Molecular Sequence Data, Protein Binding, Protein Conformation, Rec A Recombinases, Conserved Sequence, Crystallography, X-Ray

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