Creation of long-lasting blood vessels
Abstract
The construction of stable blood vessels is a fundamental challenge for tissue engineering in regenerative medicine. Although certain genes can be introduced into vascular cells to enhance their survival and proliferation, these manipulations may be oncogenic. We show here that a network of long-lasting blood vessels can be formed in mice by co-implantation of vascular endothelial cells and mesenchymal precursor cells, by-passing the need for risky genetic manipulations. These networks are stable and functional for one year in vivo.
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HUVECs have exhibited poor engraftment, limited capacity to form vessels, and an inability to survive long-term after in vivo application.
“Additionally, HUVECs have exhibited poor engraftment, limited capacity to form vessels, and an inability to survive long-term after in vivo application [237,238].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [237]
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“Additionally, HUVECs have exhibited poor engraftment, limited capacity to form vessels, and an inability to survive long-term after in vivo application [237,238]. marrow, representing approximately 0.01 % of all blood mononuclear cells, with some cells residing in the adipose tissue of adults [234].”
BackgroundInfluential
- Authors
- Koike, N., Fukumura, D., Gralla, O., Au, P., Schechner, J. S., & Jain, R. K.
- Affiliations
- Harvard University (US)
- Corresponding author
- Jain, Rakesh K.
- ORCID
- Fukumura, Dai
- Venue
- Nature
- Published
- 2004-03
- Volume
- 428
- Issue
- 6979
- Pages
- 138-139
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2089004231
- Cited by
- 701
- References
- 0
- Citations by year
- 2026: 12 · 2025: 17 · 2024: 17 · 2023: 11 · 2022: 23 · 2021: 25 · 2020: 25 · 2019: 33 · 2018: 39 · 2017: 40 · 2016: 36 · 2015: 31 · 2014: 47 · 2013: 39 · 2012: 46
- FWCI
- 29.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- National Cancer Institute (P01CA080124)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Keywords
- vascular network formation, endothelial cells, mesenchymal precursor cells, co-implantation, vascular stability, tissue engineering, regenerative medicine
- MeSH terms
- Animals, Blood Vessels, Capillary Permeability, Cell Differentiation, Cell Line, Cells, Cultured, Humans, Perfusion, Umbilical Veins, Biomarkers, Coculture Techniques, Tissue Engineering, Endothelial Cells, Regenerative Medicine, Mice, Mesenchymal Stem Cells
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