The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Functional regeneration after laser axotomy
Abstract
Understanding how nerves regenerate is an important step towards developing treatments for human neurological disease, but investigation has so far been limited to complex organisms (mouse and zebrafish) in the absence of precision techniques for severing axons (axotomy). Here we use femtosecond laser surgery for axotomy in the roundworm Caenorhabditis elegans and show that these axons functionally regenerate after the operation. Application of this precise surgical technique should enable nerve regeneration to be studied in vivo in its most evolutionarily simple form.
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“In contrast, forward locomotion in these mutants or D-ablated worms is described either as wild type (Walthall et al., 1993; Yanik et al., 2004), or nearly wild type with a reduction in amplitude (McIntire et al., 1993b).”
From Gait Modulation in C. elegans: An Integrated Neuromechanical Model (2012) · cites this paper as Yanik et al., 2004
In the citing paper
“The function of D-class neurons can be knocked out through mutation of genes required in the GABA pathway (McIntire et al., 1993b), by targeted killing of D-class neurons (Walthall et al., 1993) and by laser axotomy (Yanik et al., 2004), yielding essentially the same so-called shrinker phenotype (Hodgkin, 1983) in all cases: When touched on the head, wild type worms will back up, whereas worms who”
From Gait Modulation in C. elegans: An Integrated Neuromechanical Model (2012) · cites this paper as Yanik et al., 2004
In the citing paper
“In fact, in every case where forward locomotion of GABA pathway defective worms was reported (McIntire et al., 1993b; Walthall et al., 1993; Yanik et al., 2004), the assay was performed on agar.”
From Gait Modulation in C. elegans: An Integrated Neuromechanical Model (2012) · cites this paper as Yanik et al., 2004
Citations
Cites
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Items 501–527 of 527
Ultrafast laser micro and nano processing — the state of the art and future perspective
Sugioka, K. (2009) · 2009 Conference on Lasers & Electro Optics & The Pacific Rim Conference on Lasers and Electro-Optics · doi:10.1109/cleopr.2009.5292231
Rohde, C., Gilleland, C., Samara, C., & Yanik, M. (2009) · IEEE/NIH Life Science Systems and Applications Workshop · doi:10.1109/lissa.2009.4906707
“Another nonlinear optical technique, femtosecond-laser micro/nanosurgery enables precision ablation of sub-cellular processes with minimal collateral damage [8] and we have previously employed this technique to perform the first axonal regeneration study in C. elegans [9][ 10 ].”
MethodSelf-citationInfluence of location-dependent protuberance damage on cell viability
Yang, H.-F., Zhou, M., Di, J.-K., Zhao, E.-L., Yang, P.-F., Gong, A., & Sun, X.-L. (2009) · doi:10.1007/s11434-009-0028-3
Probing cilia-driven flow in living embryos using femtosecond laser ablation and fast imaging
Supatto, W., Fraser, S., & Vermot, J. (2009) · LASE · doi:10.1117/12.808330
“Furthermore, thanks to the use of near-infrared radiations allowing a deep penetration inside thicker specimen, it has been applied in neuroscience [25; 26], and developmental biology [27; 28; 29].”
BackgroundRohde, C., Zeng, F., Gilleland, C., Samara, C., & Yanik, M. (2009) · LASE · doi:10.1117/12.822328
“elegans [10][11], which opened up tremendous potential for genetic/drug discoveries on neural degeneration and regeneration using a genetically amenable whole-animal model.”
BackgroundSelf-citationGuo, S. X., Bourgeois, F., Chokshi, T., Durr, N., Hilliard, M., Chronis, N., & Ben-Yakar, A. (2008) · Lasers and Electro-Optics Society Meeting · doi:10.1109/leos.2008.4688576
“elegans using ultrashort laser pulses with the same high precision as we previously demonstrated [1,2], while monitoring the subsequent axonal regeneration activity.”
BackgroundMethodSelf-citationArellano, J. B., de Aldana, J. R. V., Méndez, C., González-Pérez, S., Moreno, P., & Roso, L. (2008) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2008.00388.x
Intracellular manipulation using nonlinear excitation
Watanabe, W. (2008) · 2008 Asia Optical Fiber Communication & Optoelectronic Exposition & Conference · doi:10.1364/aoe.2008.sap4
“The manipulation of subcellular structures includes optical tweezers, fluorescence recovery after photobleaching (FRAP) [6,7], intracellular ablation or nanosurgery [8-21], and photo-conversion or photo-activation [22-26].”
MethodAblation of the Mauthner Cell Lateral Dendrite Using Femtosecond Laser Pulses
Shum, J., Nishimura, N., Fetcho, J., & Schaffer, C. (2008) · doi:10.1364/fio.2008.ftul2
Rohde, C., Zeng, F., Gilleland, C., Gonzalez-Rubio, R., Angel, M., & Yanik, M. (2008) · 2008 Conference on Lasers and Electro-Optics and 2008 Conference on Quantum Electronics and Laser Science · doi:10.1109/cleo.2008.4550963
Self-citationIn vivo multi-photon nanosurgery on cortical neurons: focusing on network organization
Sacconi, L., O'Connor, R. P., Jasaitis, A., Masi, A., Buffelli, M., & Pavone, F. (2008) · SPIE BiOS · doi:10.1117/12.761588
“Multi-photon nanosurgery has been performed in worms to study axon regeneration (Yanik et al. 2004) and dissect the role of specific neurons in behaviour (Chung et al.”
BackgroundIntracellular Nanosurgery Using Near-Infrared Ultrashort Laser Pulses
Watanabe, W., Matsunaga, S., Fukui, K., & Itoh, K. (2007) · doi:10.2184/lsj.35.448
Ultrafast dynamics in a live cell irradiated by femtosecond laser pulses
Kawano, H., Hara, C., Etoh, T., & Miyawaki, A. (2007) · European Conference on Biomedical Optics · doi:10.1117/12.728045
Femtosecond Laser Near-Field Ablation by Gold Nanoparticles
Eversole, D., Guo, X., Luk'yanchuk, B., & Ben-Yakar, A. (2006) · doi:10.1364/fio.2006.fwj2
Self-citationFemtosecond Laser Nanosurgery and Study of Nerve Regeneration and Degeneration
Yanik, M. (2006) · Lasers and Electro-Optics Society Meeting · doi:10.1109/leos.2006.279138
Self-citationFemtosecond Laser Nano-Surgery for Nerve Regeneration Studies
Ben-Yakar, A., Bourgeois, F., & Hoy, C. (2006) · Lasers and Electro-Optics Society Meeting · doi:10.1109/leos.2006.278911
Self-citationSupatto, W., Débarre, D., Moulia, B., Farge, E., & Beaurepaire, E. (2006) · SPIE BiOS · doi:10.1117/12.645897
Jinshuai, 杜. D., Yin, 邓. D., Shiyang, 曹. C., Zeying, 陆. L., Jie, 李. L., Zhuo, 韩. H., Jinmin, 周. Z., Ke, 王. W., Lili, 桂. G., & Kun, 徐. X. (2025) · Infrared and Laser Engineering · doi:10.3788/irla20250073
Trends in Nanotechnology Development in Medical Applications
Chaichi, A., Sheikh, E., Mukhopadhyay, S., & Gartia, M. R. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-820352-1.00112-7 · Cited by 2
Animal models of central nervous system disorders
Gomes, E. D., Barata‐Antunes, S., Teixeira‐Castro, A., Assunção-Silva, R. C., Marques, C. R., Monteiro, S., Teixeira, F. G., Fernandes, A. M., & Silva, N. A. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818084-6.00018-0 · Cited by 3
Developing Wavefront Shaping Techniques for Focusing through Highly Dynamic Scattering Media
Hemphill, A. S. (2018) · doi:10.7936/k7js9pv1
“In both biology and medicine, light play a key role in high-resolution, high-sensitivity imaging[1]–[3] and diagnostics, as well as in the precise delivery of energy for therapeutic techniques such as optical manipulation and stimulation[4], microsurgery[5], and phototherapy[6].”
BackgroundCellular and Molecular Mechanisms of Zebrafish Fin Regeneration
McMillan, S. C. (2016) · doi:10.20381/ruor-199
“Among invertebrates, planarians and hydra can easily regenerate entire body parts (Géraudie and Ferretti, 1998; Brockes and Kumar, 2002), while C. elegans and Drosophila possess only limited regenerative capabilities (Sustar and Schubiger, 2005; Bryant, 1971; Yanik et al., 2004).”
BackgroundPhotonic-Crystal Fiber Platform for Ultrafast Optical Science
Zheltikov, A. M. (2012) · NATO science for peace and security series. B, Physics and biophysics · doi:10.1007/978-94-007-5313-6_9
Planar Cell Polarity Genes prkl-1 and dsh-1 Polarize C. Elegans Motorneurons during Organogenesis
Sánchez-Alvarez, L. (2012) · doi:10.20381/ruor-6205
“For instance, an exciting technique developed by Yanik MF et al, in 2004 (Yanik et al., 2004), based on a femtosecond infrared laser microsurgery to precisely dissect axons (axotomy), provided the foundation for screens to uncover the genetic basis of axon repair in vivo (Yanik et al.”
BackgroundMethodInfluentialLasers in Medicine and Biotechnology
Bäuerle, D. (2011) · doi:10.1007/978-3-642-17613-5_31
High-throughput, on-chip, whole-animal screening at subcellular resolution
Yanik, M. (2009) · doi:10.1117/2.1200902.1410
Self-citationLaser photoablation: a new biomedical tool
Nishimura, N. (2006) · SPIE Newsroom · doi:10.1117/2.1200608.0370
Metadata
- Authors
- Yanik, M. F., Cinar, H., Cinar, H. N., Chisholm, A. D., Jin, Y., & Ben-Yakar, A.
- Affiliations
- Stanford University (US)
- University of California, Santa Cruz (US)
- Howard Hughes Medical Institute (US)
- Venue
- Nature
- Published
- 2004-12-15
- Volume
- 432
- Issue
- 7019
- Pages
- 822-822
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2076188357
- Cited by
- 598
- References
- 0
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 15 · 2023: 21 · 2022: 15 · 2021: 30 · 2020: 38 · 2019: 23 · 2018: 20 · 2017: 25 · 2016: 37 · 2015: 21 · 2014: 24 · 2013: 35 · 2012: 45
- FWCI
- 7.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- nerve regeneration, axotomy, Caenorhabditis elegans, functional regeneration
- MeSH terms
- Animals, Axons, Larva, Locomotion, Motor Neurons, Nerve Regeneration, Neurosurgery, Caenorhabditis elegans, Axotomy, Laser Therapy
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