The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Human cells are protected from mitochondrial dysfunction by complementation of DNA products in fused mitochondria
Abstract
Extensive complementation between fused mitochondria is indicated by recombination of 'parental' mitochondrial (mt) DNA (ref. 1,2) of yeast and plant cells. It has been difficult, however, to demonstrate the occurrence of complementation between fused mitochondria in mammalian species through the presence of recombinant mtDNA molecules, because sequence of mtDNA throughout an individual tends to be uniform owing to its strictly maternal inheritance. We isolated two types of respiration-deficient cell lines, with pathogenic mutations in mitochondrial tRNAIle or tRNALeu(UUR) genes from patients with mitochondrial diseases. The coexistence of their mitochondria within hybrids restored their normal morphology and respiratory enzyme activity by 10-14 days after fusion, indicating the presence of an extensive and continuous exchange of genetic contents between the mitochondria. This complementation between fused mitochondria may represent a defence of highly oxidative organelles against mitochondrial dysfunction caused by the accumulation of mtDNA lesions with age.
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“This process enables the redistribution of energy in the form of mitochondrial potential, metabolites, proteins, and mtDNA, improving calcium handling, and ATP synthesis [24, 54–57].”
From The connection between the dynamic remodeling of the mitochondrial network and the regulation of muscle mass (2021) · cites this paper as [57]
Citations
Cites
Oxidative damage and mitochondrial decay in aging.
Shigenaga, M. K., Hagen, T. M., & Ames, B. N. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.23.10771 · Cited by 2,055
Mitochondrial Genetics: A Paradigm for Aging and Degenerative Diseases?
Wallace, D. C. (1992) · Science · doi:10.1126/science.1533953 · Cited by 1,262
Mitochondrial DNA mutations as an important contributor to ageing and degenerative diseases
Linnane, A., Ozawa, T., Marzuki, S., & Tanaka, M. (1989) · The Lancet · doi:10.1016/s0140-6736(89)92145-4 · Cited by 983
Mitochondrial DNA deletions in human brain: regional variability and increase with advanced age
Corral-Debrinski, M., Horton, T., Lott, M. T., Shoffner, J. M., Flint Beal, M., & Wallace, D. C. (1992) · Nature Genetics · doi:10.1038/ng1292-324 · Cited by 890
Seligman, A. M., Karnovsky, M. J., Wasserkrug, H. L., & Hanker, J. S. (1968) · The Journal of Cell Biology · doi:10.1083/jcb.38.1.1 · Cited by 883
Michikawa, Y., Mazzucchelli, F., Bresolin, N., Scarlato, G., & Attardi, G. M. (1999) · Science · doi:10.1126/science.286.5440.774 · Cited by 724
Hayashi, J., Ohta, S., Kikuchi, A., Takemitsu, M., Goto, Y., & Nonaka, I. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.23.10614 · Cited by 553
Self-citationElimination of paternal mitochondrial DNA in intraspecific crosses during early mouse embryogenesis.
Kaneda, H., Hayashi, J., Takahama, S., Taya, C., Lindahl, K. F., & Yonekawa, H. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.10.4542 · Cited by 462
Self-citationNakada, K., Inoue, K., Ono, T., Isobe, K., Ogura, A., Goto, Y., Nonaka, I., & Hayashi, J. (2001) · Nature Medicine · doi:10.1038/90976 · Cited by 430
Self-citationMosaicism for a specific somatic mitochondrial DNA mutation in adult human brain
Soong, N. W., Hinton, D. R., Cortopassi, G. A., & Arnheim, N. (1992) · Nature Genetics · doi:10.1038/ng1292-318 · Cited by 414
Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion
Belliard, G., VEDEL, F., & Pelletier, G. (1979) · Nature · doi:10.1038/281401a0 · Cited by 381
Inoue, K., Nakada, K., Ogura, A., Isobe, K., Goto, Y.-I., Nonaka, I., & Hayashi, J.-I. (2000) · Nature Genetics · doi:10.1038/82826 · Cited by 333
Self-citationDujon, B., Słonimski, P. P., & Weill, L. (1974) · Genetics · doi:10.1093/genetics/78.1.415 · Cited by 260
Shitara, H., Hayashi, J., Takahama, S., Kaneda, H., & Yonekawa, H. (1998) · Genetics · doi:10.1093/genetics/148.2.851 · Cited by 232
Self-citationYoneda, M., Miyatake, T., & Attardi, G. M. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.4.2699 · Cited by 193
Shitara, H., Kaneda, H., Sato, A., Inoue, K., Ogura, A., Yonekawa, H., & Hayashi, J. (2000) · Genetics · doi:10.1093/genetics/156.3.1277 · Cited by 148
Self-citationHuman mitochondria and mitochondrial genome function as a single dynamic cellular unit.
Hayashi, J.-I., Takemitsu, M., Goto, Y., & Nonaka, I. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.125.1.43 · Cited by 142
Self-citationAre mitochondrial DNA mutations involved in the carcinogenic process?
Shay, J. W., & Werbin, H. (1987) · Mutation Research/Reviews in Genetic Toxicology · doi:10.1016/0165-1110(87)90028-5 · Cited by 132
Enrı́quez, J. A., Cabezas‐Herrera, J., Bayona‐Bafaluy, M. P., & Attardi, G. M. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.15.11207 · Cited by 94
Hayashi, J., Tagashira, Y., & Yoshida, M. C. (1985) · Experimental Cell Research · doi:10.1016/0014-4827(85)90185-5 · Cited by 88
Self-citationAgeing and mammalian mitochondrial genetics
Nagley, P., & Wei, Y. (1998) · Trends in Genetics · doi:10.1016/s0168-9525(98)01580-7 · Cited by 88
Ito, S., Ohta, S., Nishimaki, K., Kagawa, Y., Soma, R., Kuno, S., Komatsuzaki, Y., Mizusawa, H., & Hayashi, J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.5.2099 · Cited by 80
Self-citationHayashi, J., Ohta, S., Kagawa, Y., Takai, D., Miyabayashi, S., Tada, K., Fukushima, H., Inui, K., Okada, S., & Goto, Y. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32274-3 · Cited by 78
Self-citationIsobe, K., Ito, S., Hosaka, H., Iwamura, Y., Kondo, H., Kagawa, Y., & Hayashi, J. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.8.4601 · Cited by 59
Self-citationMitochondrial DNA – all things bad?
Lightowlers, R. N., Jacobs, H. T., & Kajander, O. A. (1999) · Trends in Genetics · doi:10.1016/s0168-9525(98)01684-9 · Cited by 59
Nucleotide sequence identity of mitochondrial DNA from different human tissues
Monnat, R. J., & Reay, D. T. (1986) · Gene · doi:10.1016/0378-1119(86)90208-8 · Cited by 57
Inoue, K., Takai, D., Hosaka, H., Ito, S., Shitara, H., Isobe, K., Pecq, J. L., Segal-Bendirdjian, E., & Hayashi, J. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.7446 · Cited by 47
Self-citationTakai, D., Inoue, K., Goto, Y., Nonaka, I., & Hayashi, J. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.9.6028 · Cited by 43
Self-citationTissue specificity in cytochrome c oxidase deficient myopathy
Nonaka, I., Koga, Y., Ohtaki, E., & Yamamoto, M. (1989) · Journal of the Neurological Sciences · doi:10.1016/0022-510x(89)90136-6 · Cited by 33
Flagged first, then by citations
Research works printed but not linked: 1
- Takai, D., Inoue, K., Goto, Y.-I., Nonaka, I. & Hayashi, J.-I. The interorganellar interaction between distinct human mitochondria with deletion mutant mtDNA from a patient with mitochondrial disease and with HeLa mtDNA. J. Biol. Chem. 272, 6028–6033 (1997). · DOI
Cited by
Sorted by citations
Chen, H., Detmer, S. A., Ewald, A. J., Griffin, E. E., Fraser, S. E., & Chan, D. C. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200211046 · Cited by 2,601
“For example, the mitochondria of rat cardiac muscle and diaphragm skeletal muscle appear as isolated ellipses or tubules in embryonic stages but then reorganize into reticular networks in the adult (Bakeeva et al., 1978, 1981, 1983).”
BackgroundMitochondrial fusion and fission in cell life and death
Westermann, B. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3013 · Cited by 2,002
Mitochondria: Dynamic Organelles in Disease, Aging, and Development
Chan, D. C. (2006) · Cell · doi:10.1016/j.cell.2006.06.010 · Cited by 1,987
Mutations in the mitochondrial GTPase mitofusin 2 cause Charcot-Marie-Tooth neuropathy type 2A
Züchner, S., Mersiyanova, I. V., Muglia, M., Bissar-Tadmouri, N., Rochelle, J., Dadali, E. L., Zappia, M., Nelis, E., Patitucci, A., Senderek, J., et al. (2004) · Nature Genetics · doi:10.1038/ng1341 · Cited by 1,572
Disruption of Fusion Results in Mitochondrial Heterogeneity and Dysfunction
Chen, H., Chomyn, A., & Chan, D. C. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m503062200 · Cited by 1,376
Metadata
- Authors
- Ono, T., Isobe, K., Nakada, K., & Hayashi, J.-I.
- Affiliations
- University of Tsukuba (JP)
- Corresponding author
- Hayashi, Jun-Ichi
- ORCID
- Nakada, Kazuto
- Venue
- Nature Genetics
- Published
- 2001-07
- Volume
- 28
- Issue
- 3
- Pages
- 272-275
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- OpenAlex
- W1554250492
- Cited by
- 420
- References
- 29
- Citations by year
- 2026: 7 · 2025: 14 · 2024: 14 · 2023: 10 · 2022: 16 · 2021: 24 · 2020: 18 · 2019: 15 · 2018: 10 · 2017: 24 · 2016: 13 · 2015: 21 · 2014: 21 · 2013: 11 · 2012: 28
- FWCI
- 5.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- mitochondrial fusion, mitochondrial recombination, mitochondrial dysfunction, mitochondrial tRNA mutations, mitochondrial disease, respiratory deficiency, oxidative stress, human cell lines
- MeSH terms
- DNA, Mitochondrial, Genetic Complementation Test, Humans, Hybrid Cells, Intracellular Membranes, Membrane Fusion, Mitochondria, Oxygen Consumption, RNA, Transfer, Ile, RNA, Transfer, Leu, Mitochondrial Myopathies
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