The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry
Open accessAbstract
Mitochondrial membrane potential provides a valuable indicator of cells' health and functional status. Cytometry- and microscopy-based analyses, in combination with fluorescent probes, are widely used to study mitochondrial behavior related to cellular pathways, most notably - apoptosis. The cyanine dye JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimi- dazolylcarbocyanine iodide) facilitates discrimination of energized and deenergized mitochondria because the normally green fluorescent dye forms red fluorescent aggregates when concentrated in energized mitochondria in response to their higher membrane potential. JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers. In this study, we show that in practice this approach is not optimal for monitoring mitochondrial behavior. Investigation of fluorescence of JC-1 in solution and in cells using spectrofluorimetry, microscopy and flow cytometry reveals that excitation at 405 nm wavelength, now available on standard instruments, produces signals from aggregate fluorescence with considerably less spillover from dye monomer fluorescence than can be obtained using 488 nm excitation. The improved data are more accurate and eliminate the necessity for fluorescence compensation, making the use of the alternative excitation wavelengths beneficial for mitochondria-related biological and biomedial research.
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Cites
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Cited by
Tai, H., Wang, Z., Gong, H., Han, X., Zhou, J., Wang, X., Wei, X., Ding, Y., Huang, N., Qin, J., et al. (2017) · Autophagy · doi:10.1080/15548627.2016.1247143 · Cited by 334
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She, X., Khamooshi, K., Gao, Y., Shen, Y., Lv, Y., Calderone, R., Fonzi, W., Liu, W., & Li, D. (2015) · Cellular Microbiology · doi:10.1111/cmi.12438 · Cited by 51
“The fluorescent dye JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′tetraethylbenzimidazolocarbo-cyanine iodide) (Gallagher, 2010; Perelman et al., 2012) was obtained from Invitrogen, Inc.”
MethodCaicedo, A., Zambrano, K., Sanon, S., Luis Vélez, J., Montalvo, M., Jara, F., Moscoso, S. A., Vélez, P., Maldonado, A., & Velarde, G. (2021) · Mitochondrion · doi:10.1016/j.mito.2021.02.014 · Cited by 48
Gupta, P. K., Pappuru, S., Gupta, S., Patra, B., Chakraborty, D., & Verma, R. S. (2019) · Materials Science and Engineering: C · doi:10.1016/j.msec.2019.03.041 · Cited by 44
Metadata
- Authors
- Perelman, A., Wachtel, C., Cohen, M., Haupt, S., Shapiro, H., & Tzur, A.
- Venue
- Cell Death & Disease
- Published
- 2012-11-22
- Volume
- 3
- Issue
- 11
- Pages
- e430-e430
- ISSN
- 2041-4889 (ISSN-L)
- OpenAlex
- W2071007484
- Cited by
- 699
- References
- 12
- FWCI
- 5.11
- Type
- journal article
- Language
- en
- Open access
- gold
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- Perelman, A., Wachtel, C., Cohen, M., Haupt, S., Shapiro, H., & Tzur, A. (2012). JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death & Disease. https://doi.org/10.1038/cddis.2012.171
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