The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system
Open accessAbstract
Filamentous fungi have wide applications in biotechnology. The CRISPR/Cas9 system is a powerful genome-editing method that facilitates genetic alterations of genomes in a variety of organisms. However, a genome-editing approach has not been reported in filamentous fungi. Here, we demonstrated the establishment of a CRISPR/Cas9 system in the filamentous fungus Trichoderma reesei by specific codon optimization and in vitro RNA transcription. It was shown that the CRISPR/Cas9 system was controllable and conditional through inducible Cas9 expression. This system generated site-specific mutations in target genes through efficient homologous recombination, even using short homology arms. This system also provided an applicable and promising approach to targeting multiple genes simultaneously. Our results illustrate that the CRISPR/Cas9 system is a powerful genome-manipulating tool for T. reesei and most likely for other filamentous fungal species, which may accelerate studies on functional genomics and strain improvement in these filamentous fungi.
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Cites
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Miller, G. L. (1959) · Analytical Chemistry · doi:10.1021/ac60147a030 · Cited by 28,963
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
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Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
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Microbial Pigments in the Food Industry—Challenges and the Way Forward
Sen, T., Barrow, C. J., & Deshmukh, S. K. (2019) · Frontiers in Nutrition · doi:10.3389/fnut.2019.00007 · Cited by 367
Hillary, V. E., & Ceasar, S. A. (2023) · Molecular Biotechnology · doi:10.1007/s12033-022-00567-0 · Cited by 320
Genome editing in cotton with the CRISPR/cas9 system
Gao, W., Long, L., Tian, X., Xu, F., Liu, J., Singh, P. K., Botella, J. R., & Song, C. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01364 · Cited by 254
Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus
Zhang, C., Meng, X., Wei, X., & Lu, L. (2016) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2015.12.007 · Cited by 264
Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus
Fuller, K. K., Chen, S., Loros, J. J., & Dunlap, J. C. (2015) · Eukaryotic Cell · doi:10.1128/ec.00107-15 · Cited by 244
Metadata
- Authors
- Liu, R., Chen, L., Jiang, Y., Zhou, Z., & Zou, G.
- Venue
- Cell Discovery
- Published
- 2015-05-12
- Volume
- 1
- Issue
- 1
- Pages
- 15007-15007
- ISSN
- 2056-5968 (ISSN-L)
- OpenAlex
- W2529558525
- Cited by
- 456
- References
- 6
- FWCI
- 13.51
- Type
- journal article
- Language
- en
- Open access
- gold
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- Liu, R., Chen, L., Jiang, Y., Zhou, Z., & Zou, G. (2015). Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system. Cell Discovery. https://doi.org/10.1038/celldisc.2015.7
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