An efficient method for long-term room temperature storage of RNA
Open accessAbstract
RNA is a tool used in many fields, from molecular and cellular biology to medicine and nanotechnology. For most of these uses, the integrity of RNA is required and must be maintained during storage. Even though freezing is currently the storage method of choice, the increasing number of samples to be stored and the costly use of a cold chain have highlighted the need for room temperature preservation methods. Here, we report a new room temperature technology that consists in drying RNA samples in the presence of a stabilizer in stainless steel minicapsules. These air- and water-tight capsules isolate RNA from the atmosphere and maintain an anhydrous and anoxic environment. Through the evaluation of RNA integrity over time at room temperature or 90 °C, we identified atmospheric humidity as a major deleterious factor. The degradation rate dependence in temperature fitted an Arrhenius model, with an activation energy of 28.5 kcal/mol and an extrapolated room temperature degradation rate of 3.2 10(-13)/nt/s (95% confidence interval: 2.3-4.2/nt/s). In these conditions, it is expected that an RNA molecule will be subjected to 0.7-1.3 cut every 1000 nucleotides per century. In addition, we showed that stored RNA is compatible for further analyses, such as reverse transcription-quantitative PCR. No significant change in the Cq values was observed over a simulated period of several decades. At last, our data are consistent with a sequence-independent degradation rate of RNA in the solid state.
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Hydrolysis appears to be accepted as the main factor driving mRNA degradation.
“However, as stated before, hydrolysis appears to be accepted as the main factor driving mRNA degradation (Fabre et al., 2014).”
From mRNA-lipid nanoparticle COVID-19 vaccines: structure and stability (2021) · cites this paper as Fabre et al., 2014
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- Authors
- Fabre, A.-L., Colotte, M., Luis, A., Tuffet, S., & Bonnet, J.
- Affiliations
- Université de Bordeaux (FR)
- Corresponding author
- Fabre, Anne-Lise
- Venue
- European Journal of Human Genetics
- Published
- 2013-07-17
- Volume
- 22
- Issue
- 3
- Pages
- 379-385
- ISSN
- 1018-4813 (ISSN-L)
- 1476-5438
- PMC
- PMC3925273
- OpenAlex
- W2041875488
- Cited by
- 151
- References
- 34
- Citations by year
- 2026: 9 · 2025: 23 · 2024: 16 · 2023: 19 · 2022: 27 · 2021: 24 · 2020: 9 · 2019: 3 · 2018: 4 · 2017: 8 · 2016: 3 · 2015: 5 · 2014: 1
- FWCI
- 1.34
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- European Journal of Human Genetics (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- RNA storage, RNA degradation, RNA integrity, air humidity, Arrhenius model, activation energy, degradation rate
- MeSH terms
- Hot Temperature, Hydrolysis, Nucleic Acid Denaturation, RNA, Bacterial, Specimen Handling, Time Factors, RNA Stability
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