The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Arp2/3 complex interactions and actin network turnover in lamellipodia
Open accessAbstract
Cell migration is initiated by lamellipodia-membrane-enclosed sheets of cytoplasm containing densely packed actin filament networks. Although the molecular details of network turnover remain obscure, recent work points towards key roles in filament nucleation for Arp2/3 complex and its activator WAVE complex. Here, we combine fluorescence recovery after photobleaching (FRAP) of different lamellipodial components with a new method of data analysis to shed light on the dynamics of actin assembly/disassembly. We show that Arp2/3 complex is incorporated into the network exclusively at the lamellipodium tip, like actin, at sites coincident with WAVE complex accumulation. Capping protein likewise showed a turnover similar to actin and Arp2/3 complex, but was confined to the tip. In contrast, cortactin-another prominent Arp2/3 complex regulator-and ADF/cofilin-previously implicated in driving both filament nucleation and disassembly-were rapidly exchanged throughout the lamellipodium. These results suggest that Arp2/3- and WAVE complex-driven actin filament nucleation at the lamellipodium tip is uncoupled from the activities of both cortactin and cofilin. Network turnover is additionally regulated by the spatially segregated activities of capping protein at the tip and cofilin throughout the mesh.
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Cited by
Actin Dynamics, Architecture, and Mechanics in Cell Motility
Blanchoin, L., Boujemaa-Paterski, R., Sykes, C., & Plastino, J. (2014) · Physiological Reviews · doi:10.1152/physrev.00018.2013 · Cited by 1,457
The Arp2/3 Activator WASH Controls the Fission of Endosomes through a Large Multiprotein Complex
Derivery, E., Sousa, C., Gautier, J. J., Lombard, B., Loew, D., & Gautreau, A. (2009) · Developmental Cell · doi:10.1016/j.devcel.2009.09.010 · Cited by 602
Ins and outs of ADF/cofilin activity and regulation
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Inhibitory signalling to the Arp2/3 complex steers cell migration
Dang, I., Gorelik, R., Sousa-Blin, C., Derivery, E., Guérin, C., Linkner, J., Nemethova, M., Dumortier, J. G., Giger, F. A., Chipysheva, T. A., et al. (2013) · Nature · doi:10.1038/nature12611 · Cited by 276
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Metadata
- Authors
- Lai, F. P., Szczodrak, M., Block, J., Faix, J., Breitsprecher, D., Mannherz, H. G., Stradal, T. E., Dunn, G. A., Small, J. V., & Rottner, K.
- Venue
- The EMBO Journal
- Published
- 2008-02-28
- Volume
- 27
- Issue
- 7
- Pages
- 982-992
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- OpenAlex
- W2120446093
- Cited by
- 300
- References
- 50
- FWCI
- 10.51
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Lai, F. P., Szczodrak, M., Block, J., Faix, J., Breitsprecher, D., Mannherz, H. G., Stradal, T. E., Dunn, G. A., Small, J. V., & Rottner, K. (2008). Arp2/3 complex interactions and actin network turnover in lamellipodia. The EMBO Journal. https://doi.org/10.1038/emboj.2008.34
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