CoChat
Sign Up
Cited by 67Antioxidant Activity and Oxidative StressCardiovascular Health and Disease PreventionNitric Oxide and Endothelin Effects

Biomarkers of lipid peroxidation related to hypertension in aging

Open access
Yavuzer, Hakan, Yavuzer, Serap, Cengiz, Mahir, Erman, Hayriye, Doventas, Alper, Balci, Huriye, Erdincler, Deniz Suna, Uzun, Hafize·2016·Hypertension Research
DOI 10.1038/hr.2015.156
Open this paper in CoChat — free accountOpen PDF

Abstract

The aim of this clinical study was to evaluate the influence of aging on the levels of lipid peroxidation (quantified as thiobarbituric acid-reactive substances (TBARS) content), lipid hydroperoxide (LOOH), hexanoyl lysine (HEL), 8-iso-prostaglandin F2α (8-iso-PGF2α) and total antioxidant capacity (TAC), and determine their relationships to the demographic and cardiovascular risk factors in elderly hypertensive (HT) patients. This study consisted of four groups: two elderly groups with 30 HT patients (11 males, 19 females) and 30 normotensive healthy volunteers (15 males, 15 females), and two young groups with 30 HT patients (13 males, 17 females) and 30 normotensive healthy volunteers (12 males, 18 females). In the elderly control group, the TBARS, LOOH, HEL and 8-iso-PGF2α levels, and the carotid intima media thickness (CIMT) were significantly higher than in the young control group. The TBARS, LOOH, HEL and 8-iso-PGF2α levels and the CIMT measurements were significantly higher in the elderly HT group than in the young HT group. In addition, the TAC levels were significantly lower in the elderly and young HT groups than in the elderly and young control groups. The CIMT was significantly positively correlated with TBARS (r=0.40, P<0.001), HEL (r= 0.30, P=0.001), LOOH (r= 0.44, P<0.001) and 8-iso-PGF2α (r= 0.32, P<0.001) in all of the HT groups. It seems that in elderly patients, the LOOH and TBARS are better biomarkers of lipid peroxidation in hypertension in terms of sensitivity. In all of the HT groups, 8-iso-PGF2α had the highest sensitivity. Hypertension is associated with lipid peroxidation due to an impaired oxidant/antioxidant status. Increased lipid peroxidation and decreased antioxidants with aging indicate that peroxidative damage further increases with higher blood pressure and the aging process.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/hr.2015.156

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Serum MDA levels are higher in elderly people (age ≤ 60 years; n = 30 per group) than in adults aged 20-50 years, as measured by TBARS assay.

    “| Aging | Serum | Adults (Age 20–50 years) vs. elderly (Age ≤ 60 years, n = 30 per group) | TBARS assay | Higher MDA levels in elderly | [242] |”

    From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [242]

    The Discussion reports higher TBARS levels in elderly than young hypertensive groups, and the serum-TBARS methods section confirms the assay; Table 1 lists 30 people per subgroup and young participants aged 20–50. However, the paper reports TBARS rather than directly measured serum MDA, and Table 1 gives elderly mean ages of 69.5 and 72.1 years, inconsistent with the claim’s “age ≤60 years.” Page numbers are unavailable in our copy. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 36

  1. Saxena R. Arthritis as a disease of ageing and changes in antioxidant status. In: Preedy VR (ed), Aging: Oxidative Stress and Dietary Antioxidants, (1st edn. Academic Press Elsevier Publications: London, 2014, 49-59.
  2. Sakai K, Kino S, Masuda A, Takeuchi M, Ochi T, Osredkar J, Rejc B, Gersak K, Ramarathnam N, Kato Y. Determination of HEL (Hexanoyl-lysine adduct): a novel biomarker for omega-6 PUFA oxidation. Subcell Biochem 2014; 77: 61-72.
  3. Basu S. Fatty acid oxidation and isoprostanes: oxidative strain and oxidative stress. Prostaglandins Leukot Essent Fatty Acids 2010; 82: 219-225.
  4. Sies H. Total antioxidant capacity: appraisal of a concept. J Nutr 2007; 137: 1493-1495.
  5. Kong X, Jia X, Wei Y, Cui M, Wang Z, Tang L, Li W, Zhu Z, Chen P, Xu D. Association between microalbuminuria and subclinical atherosclerosis evaluated by carotid artery intimamedia in elderly patients with normal renal function. BMC Nephrol 2012; 13: 37-39.
  6. Xie W, Liu J, Wang W, Wang M, Li Y, Sun J, Liu J, Qi Y, Zhao F, Zhao D. Five-year change in systolic blood pressure is independently associated with carotid athero- sclerosis progression: a population-based cohort study. Hypertens Res 2014; 37: 960-965.
  7. Reaven PD, Napoli C, Merat S, Witztumc JL. Lipoprotein modification and athero- sclerosis in aging. Exp Gerontol 2000; 34: 527-537.
  8. Seres I, Paragh G, Deschene E, Fulop T Jr, Khalil A. Study of factors influencing the decreased HDL associated PON1 activity with aging. Exp Gerontol 2004; 39: 59-66.
  9. Dincer Y, Sekercioglu N, Pekpak M, Gunes KN, Akcay T. Assessment of DNA oxidation and antioxidant activity in hypertensive patients with chronic kidney disease. Ren Fail 2008; 30: 1006-1011.
  10. Yoo SM, Choi SH, Jung MD, Lim SC, Baek SH. Short-term use of telmisartan attenuates oxidation and improves Prdx2 expression more than antioxidant β-blockers in the cardiovascular systems of spontaneously hypertensive rats. Hypertens Res 2015; 38: 106-115.
  11. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302-310.
  12. Benzi IFF, Strain JJ. Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measure- ment of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999; 299: 15-27.
  13. Uzun H, Karter Y, Aydin S, Curgunlu A, Simşek G, Yücel R, Vehiyd S, Ertürk N, Kutlu A, Benian A, Yaldiran A, Oztürk E, Erdine S. Oxidative stress in white coat hypertension; role of paraoxonase. J Hum Hypertens 2004; 18: 523-528.
  14. Dominguez LJ, Galioto A, Pineo A, Ferlisi A, Ciaccio M, Putignano E, Belvedere M, Costanza G, Barbagallo M. Age, homocysteine, and oxidative stress: relation to hypertension and type 2 diabetes mellitus. J Am Coll Nutr 2010; 29: 1-6.
  15. Kedziora-Kornatowska K, Szewczyk-Golec K, Czuczejko J, Pawluk H, van Marke de Lumen K, Kozakiewicz M, Bartosz G, Kedziora J. Antioxidative effects of melatonin administration in elderly primary essential hypertension patients. J Pineal Res 2008; 45: 312-317.
  16. Sato K, Dohi Y, Kojima M, Miyagawa K, Takase H, Katada E, Suzuki S. Effects of ascorbic acid on ambulatory blood pressure in elderly patients with refractory hypertension. Arzneimittelforschung 2006; 56: 535-540.
  17. Armas-Padilla MC, Armas-Hernández MJ, Sosa-Canache B, Cammarata R, Pacheco B, Guerrero J, Carvajal AR, Hernández-Hernández R, Israili ZH, Valasco M. Nitric oxide and malondialdehyde in human hypertension. Am J Ther 2007; 14: 172-176.
  18. Akila VP, Harishchandra H, D'souza V, D'souza B. Age related changes in lipid peroxidation and antioxidants in elderly people. Indian J Clin Biochem. 2007; 22: 131-134.
  19. Ahmad A, Singhal U, Hossain MM, Islam N, Rizvi I. The role of the endogenous antioxidant enzymes and malondialdehyde in essential hypertension. J Clin Diagn Res 2013; 7: 987-990.
  20. Kedziora-Kornatowska K, Czuczejko J, Pawluk H, Kornatowski T, Motyl J, Szadujkis- Szadurski L, Szewczyk-Golec K, Kedziora J. The markers of oxidative stress and activity of the antioxidant system in the blood of elderly patients with essential arterial hypertension. Cell Mol Biol Lett 2004; 9: 635-641.
  21. Tabak O, Gelisgen R, Erman H, Erdenen F, Muderrisoglu C, Aral H, Uzun H. Oxidative lipid, protein, and DNA damage as oxidative stress markers in vascular complications of diabetes mellitus. Clin Invest Med 2011; 34: E163-E171.
  22. Kato Y, Mori Y, Makino Y, Morimitsu Y, Hiroi S, Ishikawa T, Osawa T. Formation of Nepsilon-(hexanonyl)lysine in protein exposed to lipid hydroperoxide. A plausible marker for lipid hydroperoxide-derived protein modification. J Biol Chem 1999; 274: 20406-20414.
  23. Ichikawa D, Kamijo-Ikemori A, Sugaya T, Yasuda T, Hoshino S, Igarashi-Migitaka J, Hirata K, Kimura K. Renal liver-type fatty acid binding protein attenuates angiotensin II- induced renal injury. Hypertension 2012; 60: 973-980.
  24. Hozawa A, Ebihara S, Ohmori K, Kuriyama S, Ugajin T, Koizumi Y, Suzuki Y, Matsui T, Arai H, Tsubono Y, Sasaki H, Tsuji I. Increased plasma 8-isoprostane levels in hypertensive subjects: the Tsurugaya Project. Hypertens Res 2004; 27: 557-561.
  25. de Faria AP, Fontana V, Modolo R, Barbaro NR, Sabbatini AR, Pansani IF, Ferreira-Melo SE, Moreno H. Plasma 8-isoprostane levels are associated with endothe- lial dysfunction in resistant hypertension. Clin Chim Acta 2014; 433: 179-183.
  26. Rodrigo R, Prat H, Passalacqua W, Araya J, Guichard C, Bächler JP. Relationship between oxidative stress and essential hypertension. Hypertens Res 2007; 30: 1159-1167.
  27. Wang B, Zhu H, Sun H, Pan J, Yuan Z, Yu R. Plasma 8-isoprostane concentrations in patients with age-related cataracts. Clin Chem 2005; 51: 1541-1544.
  28. Ito N, Ohishi M, Yamamoto K, Tatara Y, Shiota A, Hayashi N, Komai N, Yanagitani Y, Rakugi H, Ogihara T. Renin-angiotensin inhibition reverses advanced cardiac remodeling in aging spontaneously hypertensive rats. Am J Hypertens 2007; 20: 792-799.
  29. Da Cruz AC, Petronilho F, Heluany CC, Vuolo F, Miguel SP, Quevedo J, Romano-Silva MA, Dal-Pizzol F. Oxidative stress and aging: correlation with clinical parameters. Aging Clin Exp Res 2014; 26: 7-12.
  30. Okoduwa SI, Umar IA, Ibrahim S, Bello F, Habila N. Age-dependent alteration of antioxidant defense system in hypertensive and type-2 diabetes patients. J Diabetes Metab Disord 2015; 14: 32.
  31. Goraca A. Assessment of total antioxidant capacity in human plasma. Folia Med (Plovdiv) 2004; 46: 16-21.
  32. Cao G, Russell RM, Lischner N, Prior RL. Serum antioxidant capacity is increased by consumption of strawberries, spinach, red wine or vitamin C in elderly women. J Nutr 1998; 128: 2383-2390.
  33. Gawron-Skarbek A, Chrzczanowicz J, Kostka J, Nowak D, Drygas W, Jegier A, Kostka T. Cardiovascular risk factors and total serum antioxidant capacity in healthy men and in men with coronary heart disease. Biomed Res Int 2014; 2014: 216964.
  34. Iversen PO, Ha L, Blomhoff R, Hauge T, Veierød MB. Baseline oxidative defense and survival after 5-7 years among elderly stroke patients at nutritional risk: Follow-up of a randomized, nutritional intervention trial. Clin Nutr 2015; 34: 775-778.
  35. Fusco D, Colloca G, Lo Monaco MR, Cesari M. Effects of antioxidant supplementation on the aging process. Clin Interv Aging 2007; 2: 377-387.
  36. Magableh MR, Kemp-Harper BK, Hart JL. Hydrogen sulfide treatment reduces blood pressure and oxidative stress in angiotensin II-induced hypertensive mice. Hypertens Res 2015; 38: 13-20.

Cited by

Sorted by citations

Authors
Yavuzer, H., Yavuzer, S., Cengiz, M., Erman, H., Doventas, A., Balci, H., Erdincler, D. S., & Uzun, H.
Affiliations
  • Istanbul University-Cerrahpaşa (TR)
  • Istanbul Medeniyet University (TR)
Corresponding author
Uzun, Hafize
ORCID
Venue
Hypertension Research
Publisher
Springer Science and Business Media LLC
Published
2016-01-14
Volume
39
Issue
5
Pages
342-348
ISSN
  • 0916-9636 (ISSN-L)
  • 1348-4214
DOI
10.1038/hr.2015.156
OpenAlex
W2233066573
Cited by
67
References
2
Citations by year
2026: 1 · 2025: 10 · 2024: 10 · 2023: 7 · 2022: 8 · 2021: 4 · 2020: 11 · 2019: 4 · 2018: 6 · 2017: 4 · 2016: 2
FWCI
4.21
Type
journal article
Language
en
Open access
Licence
Locations
  • Hypertension Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Health and Disease Prevention
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
lipid peroxidation, hypertension, aging, elderly patients, TBARS, lipid hydroperoxides, 8-isoprostane, total antioxidant capacity, carotid intima-media thickness, biomarkers, oxidant-antioxidant status
MeSH terms
Adult, Aged, Aged, 80 and over, Aging, Female, Humans, Hypertension, Lipid Peroxides, Male, Middle Aged, Risk Factors, Sensitivity and Specificity, Lipid Peroxidation, Dinoprost, Biomarkers, Thiobarbituric Acid Reactive Substances, Risk Assessment, Carotid Intima-Media Thickness, Healthy Volunteers

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review