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GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes
Abstract
Owing to their vast diversity and as-yet uncultivated status, detection, characterization and quantification of microorganisms in natural settings are very challenging, and linking microbial diversity to ecosystem processes and functions is even more difficult. Microarray-based genomic technology for detecting functional genes and processes has a great promise of overcoming such obstacles. Here, a novel comprehensive microarray, termed GeoChip, has been developed, containing 24 243 oligonucleotide (50 mer) probes and covering >10 000 genes in >150 functional groups involved in nitrogen, carbon, sulfur and phosphorus cycling, metal reduction and resistance, and organic contaminant degradation. The developed GeoChip was successfully used for tracking the dynamics of metal-reducing bacteria and associated communities for an in situ bioremediation study. This is the first comprehensive microarray currently available for studying biogeochemical processes and functional activities of microbial communities important to human health, agriculture, energy, global climate change, ecosystem management, and environmental cleanup and restoration. It is particularly useful for providing direct linkages of microbial genes/populations to ecosystem processes and functions.
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Cited by
Van Der Heijden, M. G. A., Bardgett, R. D., & Van Straalen, N. M. (2008) · Ecology Letters · doi:10.1111/j.1461-0248.2007.01139.x · Cited by 5,053
Stochastic Community Assembly: Does It Matter in Microbial Ecology?
Zhou, J., & Ning, D. (2017) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00002-17 · Cited by 2,773
Self-citationZhou, J., He, Z., Yang, Y., Deng, Y., Tringe, S. G., & Alvarez-Cohen, L. (2015) · mBio · doi:10.1128/mbio.02288-14 · Cited by 474
Self-citationRocca, J. D., Hall, E. K., Lennon, J. T., Evans, S. E., Waldrop, M. P., Cotner, J. B., Nemergut, D. R., Graham, E. B., & Wallenstein, M. D. (2015) · The ISME Journal · doi:10.1038/ismej.2014.252 · Cited by 398
A practical guide to the oral microbiome and its relation to health and disease
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“, 2002) and GeoChip for functional analysis (He et al., 2007).”
Method
Metadata
- Authors
- He, Z., Gentry, T. J., Schadt, C. W., Wu, L., Liebich, J., Chong, S. C., Huang, Z., Wu, W., Gu, B., Jardine, P., Criddle, C., & Zhou, J.
- Venue
- The ISME Journal
- Publisher
- Oxford University Press (OUP)
- Published
- 2007-05-01
- Volume
- 1
- Issue
- 1
- Pages
- 67-77
- ISSN
- 1751-7362 (ISSN-L)
- 1751-7370
- OpenAlex
- W2145132040
- Cited by
- 583
- References
- 0
- FWCI
- 46.06
- Type
- journal article
- Language
- en
- Open access
- closed
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- He, Z., Gentry, T. J., Schadt, C. W., Wu, L., Liebich, J., Chong, S. C., Huang, Z., Wu, W., Gu, B., Jardine, P., Criddle, C., & Zhou, J. (2007). GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes. The ISME Journal. https://doi.org/10.1038/ismej.2007.2
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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