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Cited by 583

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GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes

He, Zhili, Gentry, Terry J, Schadt, Christopher W, Wu, Liyou, Liebich, Jost, Chong, Song C, Huang, Zhijian, Wu, Weimin, Gu, Baohua, Jardine, Phil, Criddle, Craig, Zhou, Jizhong·2007·The ISME Journal

Abstract

Owing to their vast diversity and as-yet uncultivated status, detection, characterization and quantification of microorganisms in natural settings are very challenging, and linking microbial diversity to ecosystem processes and functions is even more difficult. Microarray-based genomic technology for detecting functional genes and processes has a great promise of overcoming such obstacles. Here, a novel comprehensive microarray, termed GeoChip, has been developed, containing 24 243 oligonucleotide (50 mer) probes and covering >10 000 genes in >150 functional groups involved in nitrogen, carbon, sulfur and phosphorus cycling, metal reduction and resistance, and organic contaminant degradation. The developed GeoChip was successfully used for tracking the dynamics of metal-reducing bacteria and associated communities for an in situ bioremediation study. This is the first comprehensive microarray currently available for studying biogeochemical processes and functional activities of microbial communities important to human health, agriculture, energy, global climate change, ecosystem management, and environmental cleanup and restoration. It is particularly useful for providing direct linkages of microbial genes/populations to ecosystem processes and functions.

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Metadata

Authors
He, Z., Gentry, T. J., Schadt, C. W., Wu, L., Liebich, J., Chong, S. C., Huang, Z., Wu, W., Gu, B., Jardine, P., Criddle, C., & Zhou, J.
Venue
The ISME Journal
Published
2007-05-01
Volume
1
Issue
1
Pages
67-77
ISSN
  • 1751-7362 (ISSN-L)
  • 1751-7370
OpenAlex
W2145132040
Cited by
583
References
0
FWCI
46.06
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes. https://app.cochat.ai/papers/10.1038/ismej.2007.2
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He, Z., Gentry, T. J., Schadt, C. W., Wu, L., Liebich, J., Chong, S. C., Huang, Z., Wu, W., Gu, B., Jardine, P., Criddle, C., & Zhou, J. (2007). GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes. The ISME Journal. https://doi.org/10.1038/ismej.2007.2

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