The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
A novel ecological role of the Firmicutes identified in thermophilic microbial fuel cells
Abstract
Significant effort is currently focused on microbial fuel cells (MFCs) as a source of renewable energy. Most studies concentrate on operation at mesophilic temperatures. However, anaerobic digestion studies have reported on the superiority of thermophilic operation and demonstrated a net energy gain in terms of methane yield. As such, our studies focused on MFC operation and microbiology at 55 °C. Over a 100-day operation, these MFCs were stable and achieved a power density of 37 mW m−2 with a coulombic efficiency of 89%. To infer activity and taxonomic identity of dominant members of the electricity-producing community, we performed phylogenetic microarray and clone library analysis with small subunit ribosomal RNA (16S rRNA) and ribosomal RNA gene (16S rDNA). The results illustrated the dominance (80% of clone library sequences) of the Firmicutes in electricity production. Similarly, rRNA sequences from Firmicutes accounted for 50% of those taxa that increased in relative abundance from current-producing MFCs, implying their functional role in current production. We complemented these analyses by isolating the first organisms from a thermophilic MFC. One of the isolates, a Firmicutes Thermincola sp. strain JR, not only produced more current than known organisms (0.42 mA) in an H-cell system but also represented the first demonstration of direct anode reduction by a member of this phylum. Our research illustrates the importance of using a variety of molecular and culture-based methods to reliably characterize bacterial communities. Consequently, we revealed a previously unidentified functional role for Gram-positive bacteria in MFC current generation.
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Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
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Self-citationExtracellular electron transfer via microbial nanowires
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Electricity Production by Geobacter sulfurreducens Attached to Electrodes
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Microorganisms pumping iron: anaerobic microbial iron oxidation and reduction
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Self-citationGorby, Y. A., Yanina, S., McLean, J. S., Rosso, K. M., Moyles, D., Dohnalkova, A., Beveridge, T. J., Chang, I. S., Kim, B. H., Kim, K. S., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604517103 · Cited by 1,749
Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells
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Dissimilatory Fe(III) and Mn(IV) Reduction
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Electrode-Reducing Microorganisms That Harvest Energy from Marine Sediments
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Experimental factors affecting PCR-based estimates of microbial species richness and evenness
Engelbrektson, A., Kunin, V., Wrighton, K. C., Zvenigorodsky, N., Chen, F., Ochman, H., & Hugenholtz, P. (2010) · The ISME Journal · doi:10.1038/ismej.2009.153 · Cited by 593
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Metadata
- Authors
- Wrighton, K. C., Agbo, P., Warnecke, F., Weber, K. A., Brodie, E. L., DeSantis, T. Z., Hugenholtz, P., Andersen, G. L., & Coates, J. D.
- Venue
- The ISME Journal
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-09-04
- Volume
- 2
- Issue
- 11
- Pages
- 1146-1156
- ISSN
- 1751-7362 (ISSN-L)
- 1751-7370
- OpenAlex
- W2114250298
- Cited by
- 353
- References
- 45
- FWCI
- 12.86
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wrighton, K. C., Agbo, P., Warnecke, F., Weber, K. A., Brodie, E. L., DeSantis, T. Z., Hugenholtz, P., Andersen, G. L., & Coates, J. D. (2008). A novel ecological role of the Firmicutes identified in thermophilic microbial fuel cells. The ISME Journal. https://doi.org/10.1038/ismej.2008.48
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