The DOI matches its Crossref record; 151 of the 152 references it cites were found in a registry or an index.
Integration-deficient Lentiviral Vectors: A Slow Coming of Age
Open accessAbstract
Lentiviral vectors are very efficient at transducing dividing and quiescent cells, which makes them highly useful tools for genetic analysis and gene therapy. Traditionally this efficiency was considered dependent on provirus integration in the host cell genome; however, recent results have challenged this view. So called integration-deficient lentiviral vectors (IDLVs) can be produced through the use of integrase mutations that specifically prevent proviral integration, resulting in the generation of increased levels of circular vector episomes in transduced cells. These lentiviral episomes lack replication signals and are gradually lost by dilution in dividing cells, but are stable in quiescent cells. Compared to integrating lentivectors, IDLVs have a greatly reduced risk of causing insertional mutagenesis and a lower risk of generating replication-competent recombinants (RCRs). IDLVs can mediate transient gene expression in proliferating cells, stable expression in nondividing cells in vitro and in vivo, specific immune responses, RNA interference, homologous recombination (gene repair, knock-in, and knock-out), site-specific recombination, and transposition. IDLVs can be converted into replicating episomes, suggesting that if a clinically applicable system can be developed they would also become highly appropriate for stable transduction of proliferating tissues in therapeutic applications.
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“Thus, NILVs are still the best option for quiescent cells, as critically summarised in one review paper [75].”
From Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through (2022) · cites this paper as [75]
In the citing paper
“This approach could help to increase the episomal gene expression, as reported in a previous study where different mutation sites of the integrase plasmid resulted in different gene expression levels [75].”
From Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through (2022) · cites this paper as [75]
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221
In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996) · Science · doi:10.1126/science.272.5259.263 · Cited by 4,903
A Third-Generation Lentivirus Vector with a Conditional Packaging System
Dull, T., Zufferey, R., Kelly, M., Mandel, R. J., Nguyen, M., Trono, D., & Naldini, L. (1998) · Journal of Virology · doi:10.1128/jvi.72.11.8463-8471.1998 · Cited by 3,341
Safety and Efficacy of Gene Transfer for Leber's Congenital Amaurosis
Maguire, A. M., Simonelli, F., Pierce, E. A., Pugh, E. N., Mingozzi, F., Bennicelli, J., Banfi, S., Marshall, K. A., Testa, F., Surace, E. M., et al. (2008) · New England Journal of Medicine · doi:10.1056/nejmoa0802315 · Cited by 2,145
Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo
Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L., & Trono, D. (1997) · Nature Biotechnology · doi:10.1038/nbt0997-871 · Cited by 1,977
Effect of gene therapy on visual function in Leber’s congenital amaurosis
Bainbridge, J. W. B., Smith, A. J., Barker, S. S., Robbie, S., Henderson, R., Balaggan, K., Viswanathan, A., Holder, G. E., Stockman, A., Tyler, N., et al. (2008) · New England Journal of Medicine · doi:10.1056/nejmoa0802268 · Cited by 1,924
Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.
Kim, Y. G., Cha, J., & Chandrasegaran, S. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.3.1156 · Cited by 1,889
A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency
Hacein-Bey-Abina, S., von Kalle, C., Schmidt, M., Le Deist, F., Wulffraat, N., McIntyre, E., Radford, I., Villeval, J.-L., Fraser, C. C., Cavazzana-Calvo, M., et al. (2003) · New England Journal of Medicine · doi:10.1056/nejm200301163480314 · Cited by 1,867
Self-Inactivating Lentivirus Vector for Safe and Efficient In Vivo Gene Delivery
Zufferey, R., Dull, T., Mandel, R. J., Bukovsky, A., Quiroz, D., Naldini, L., & Trono, D. (1998) · Journal of Virology · doi:10.1128/jvi.72.12.9873-9880.1998 · Cited by 1,815
An Adenovirus Mutant That Replicates Selectively in p53- Deficient Human Tumor Cells
Bischoff, J. R., Kirn, D. H., Williams, A., Heise, C. C., Horn, S. T., Muna, M., Ng, L., Nye, J. A., Sampson-Johannes, A., Fattaey, A., et al. (1996) · Science · doi:10.1126/science.274.5286.373 · Cited by 1,769
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Items 201–222 of 222
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Schenkwein, D. (2013) · UEF eRepo (University of Eastern Finland)
Genetic causes and stem-cell-based therapeutic strategies in neuromuscular diseases
Guo, L. (2021) · Research Publications (Maastricht University) · doi:10.26481/dis.20210623lg
Hildebrand, S., & Pfeifer, A. (2021) · Springer eBooks · doi:10.1007/978-3-030-57401-7_63
Unlocking effective transgene expression potential in gene therapy with viral vectors
Halim, N. S. S. A., Halim, N. H. A., Vengidasan, L., Zakaria, N., Ismail, I. S., & Yahaya, B. H. (2021) · Biomedical Research and Therapy · doi:10.15419/bmrat.v8i9.695
Hildebrand, S., & Pfeifer, A. (2021) · Springer eBooks · doi:10.1007/978-3-030-21573-6_63-1
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Kullmann, D. M. (2024) · doi:10.1093/med/9780197549469.003.0077
Turning Tables for CRISPR/Cas9 Editing System: From Scratch to Advanced Delivery Platforms
Chira, S., Nutu, A. M., Bica, C., Pop, L., Gherman, M., Angheluță, M.-D., & Berindan‐Neagoe, I. (2025) · Springer eBooks · doi:10.1007/978-3-032-00765-0_292
Properties and Application of Pseudotyped Lentiviral Vectors
Matsushita, N., Takada, M., & Kobayashi, K. (2025) · Methods in molecular biology · doi:10.1007/978-1-0716-4807-0_1
Lentiviral vectors for treatment of haemophilia
Ward, N. J. (2010) · UCL Discovery (University College London)
Analyse des réponses cellulaires induites par l’intégration d’un ADN étranger au sein du génome
Gay, V. (2010) · theses.fr (ABES) · doi:10.70675/7c4ae339z19ddz46b4zbd54zc859435d8459
CRISPR-Cas9-based strategies for enhanced targeted integration
Lacombe, L. (2024) · theses.fr (ABES) · doi:10.70675/9c9d279dz512az4b72z8322zea205cef9b67
Rational Design and Optimisation of CRISPR‐Cas9 Delivery Systems for Targeted Genomic Transformation
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Gene therapy development for retinal diseases
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Bioengineering strategies for prenatal therapy
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Metadata
- Authors
- Wanisch, K., & Yáñez-Muñoz, R. J.
- Affiliations
- University of London (GB)
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2009-08
- Volume
- 17
- Issue
- 8
- Pages
- 1316-1332
- ISSN
- 1525-0016 (ISSN-L)
- PMC
- PMC2835240
- OpenAlex
- W1976244438
- Cited by
- 222
- References
- 152
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 10 · 2023: 12 · 2022: 12 · 2021: 15 · 2020: 4 · 2019: 8 · 2018: 10 · 2017: 13 · 2016: 14 · 2015: 13 · 2014: 22 · 2013: 19 · 2012: 18
- FWCI
- 1.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Therapy (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Keywords
- integrase mutations, proviral integration, insertional mutagenesis, quiescent cells, transient expression, stable transduction, homologous recombination, site-specific recombination, transposition, gene therapy
- MeSH terms
- Animals, Genetic Vectors, Humans, Mutation, Transduction, Genetic, Lentivirus, Integrases
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