Integrase-defective lentiviral vectors: Progress and applications

Banasik, M B; McCray, P B

doi:10.1038/gt.2009.135

Cited by 129Virus-based gene therapy researchRNA Interference and Gene DeliveryViral Infectious Diseases and Gene Expression in Insects

Integrase-defective lentiviral vectors: Progress and applications

Banasik, M B, McCray, P B·2010·Gene Therapy

DOI 10.1038/gt.2009.135

Open this paper in CoChat — free account

Abstract

Lentiviral vectors (LVs) offer the advantages of a large packaging capacity, broad cell tropism or specific cell-type targeting through pseudotyping, and long-term expression from integrated gene cassettes. However, transgene integration carries a risk of disrupting gene expression through insertional mutagenesis and may not be required for all applications. A non-integrating LV may be beneficial in cases in which transient gene expression is desired. Several recent publications outline the development and initial biological characterization of such vectors. Here, we discuss the potential applications and new directions for the development of integration-defective LVs.

Integrity signals

Registry

Crossref · 10.1038/gt.2009.135

Cites

86 of 86 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Class I mutations are limited in affecting integrase multimerisation, linear episome processing, and DNA binding.
“In contrast, class I mutations are limited in affecting the integrase multimerisation, linear episome processing, and DNA binding [25], as listed in Table 3.”
From Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through (2022) · cites this paper as [25]
Class II mutations can be performed by exchanging the standard gag or pol packaging plasmid with an IN mutant strain.
“Class II mutations can be performed by exchanging the standard gag or pol packaging plasmid with an IN mutant strain [25].”
From Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through (2022) · cites this paper as [25]

LMO2 -Associated Clonal T Cell Proliferation in Two Patients after Gene Therapy for SCID-X1
Hacein-Bey-Abina, S., Von Kalle, C., Schmidt, M., McCormack, M. P., Wulffraat, N., Leboulch, P., Lim, A., Osborne, C. S., Pawliuk, R., Morillon, E., et al. (2003) · Science · doi:10.1126/science.1088547 · Cited by 3,587 · see citing works
A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency
Hacein-Bey-Abina, S., von Kalle, C., Schmidt, M., Le Deist, F., Wulffraat, N., McIntyre, E., Radford, I., Villeval, J.-L., Fraser, C. C., Cavazzana-Calvo, M., et al. (2003) · New England Journal of Medicine · doi:10.1056/nejm200301163480314 · Cited by 1,867 · see citing works
Insertional oncogenesis in 4 patients after retrovirus-mediated gene therapy of SCID-X1
Hacein-Bey-Abina, S., Garrigue, A., Wang, G. P., Soulier, J., Lim, A., Morillon, E., Clappier, E., Caccavelli, L., Delabesse, E., Beldjord, K., et al. (2008) · Journal of Clinical Investigation · doi:10.1172/jci35700 · Cited by 1,854 · see citing works
HIV-1 Integration in the Human Genome Favors Active Genes and Local Hotspots
Schröder, A. R. W., Shinn, P., Chen, H., Berry, C., Ecker, J. R., & Bushman, F. (2002) · Cell · doi:10.1016/s0092-8674(02)00864-4 · Cited by 1,832 · see citing works
Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector.
Naldini, L., Blömer, U., Gage, F. H., Trono, D., & Verma, I. M. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.21.11382 · Cited by 1,499 · see citing works
Transcription Start Regions in the Human Genome Are Favored Targets for MLV Integration
Wu, X., Li, Y., Crise, B., & Burgess, S. M. (2003) · Science · doi:10.1126/science.1083413 · Cited by 1,305 · see citing works
Insertional mutagenesis combined with acquired somatic mutations causes leukemogenesis following gene therapy of SCID-X1 patients
Howe, S. J., Mansour, M. R., Schwarzwaelder, K., Bartholomae, C., Hubank, M., Kempski, H., Brugman, M. H., Pike-Overzet, K., Chatters, S. J., de Ridder, D., et al. (2008) · Journal of Clinical Investigation · doi:10.1172/jci35798 · Cited by 1,286 · see citing works
Sustained Correction of X-Linked Severe Combined Immunodeficiency by ex Vivo Gene Therapy
Hacein‐Bey‐Abina, S., Deist, F. L., Carlier, F., Bouneaud, C., Hue, C., de Villartay, J., Thrasher, A. J., Wulffraat, N. M., Sorensen, R., Dupuis‐Girod, S., et al. (2002) · New England Journal of Medicine · doi:10.1056/nejmoa012616 · Cited by 1,094 · see citing works
Retroviral DNA Integration: ASLV, HIV, and MLV Show Distinct Target Site Preferences
Mitchell, R., Beitzel, B., Schroder, A. R. W., Shinn, P., Chen, H., Berry, C., Ecker, J., & Bushman, F. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020234 · Cited by 1,024 · see citing works
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery
Lombardo, A., Genovese, P., Beausejour, C. M., Colleoni, S., Lee, Y.-L., Kim, K. A., Ando, D., Urnov, F. D., Galli, C., Gregory, P. D., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1353 · Cited by 845 · see citing works
Rapid “Open-Source” Engineering of Customized Zinc-Finger Nucleases for Highly Efficient Gene Modification
Maeder, M. L., Thibodeau-Beganny, S., Osiak, A., Wright, D. A., Anthony, R. M., Eichtinger, M., Jiang, T., Foley, J. E., Winfrey, R. J., Townsend, J. A., et al. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.06.016 · Cited by 708 · see citing works
Self-citation
The genotoxic potential of retroviral vectors is strongly modulated by vector design and integration site selection in a mouse model of HSC gene therapy
Montini, E., Cesana, D., Schmidt, M., Sanvito, F., Bartholomae, C. C., Ranzani, M., Benedicenti, F., Sergi, L. S., Ambrosi, A., Ponzoni, M., et al. (2009) · Journal of Clinical Investigation · doi:10.1172/jci37630 · Cited by 571 · see citing works
Residues Critical for Retroviral Integrative Recombination in a Region That Is Highly Conserved among Retroviral/Retrotransposon Integrases and Bacterial Insertion Sequence Transposases
Kulkosky, J., Jones, K. S., Katz, R. A., Mack, J. P. G., & Skalka, A. M. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.5.2331 · Cited by 567 · see citing works
THE RETROVIRAL ENZYMES
Katz, R. A., & Skalka, A. M. (1994) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.63.070194.001025 · Cited by 524 · see citing works
Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro
Engelman, A., & Craigie, R. (1992) · Journal of Virology · doi:10.1128/jvi.66.11.6361-6369.1992 · Cited by 519 · see citing works
Effective gene therapy with nonintegrating lentiviral vectors
Yáñez-Muñoz, R. J., Balaggan, K. S., MacNeil, A., Howe, S. J., Schmidt, M., Smith, A. J., Buch, P., MacLaren, R. E., Anderson, P. N., Barker, S. E., et al. (2006) · Nature Medicine · doi:10.1038/nm1365 · Cited by 460 · see citing works
HIV integration site selection: Analysis by massively parallel pyrosequencing reveals association with epigenetic modifications
Wang, G. P., Ciuffi, A., Leipzig, J., Berry, C. C., & Bushman, F. D. (2007) · Genome Research · doi:10.1101/gr.6286907 · Cited by 457 · see citing works
Systematic Determination of the Packaging Limit of Lentiviral Vectors
Kumar, M., Keller, B. C., Makalou, N., & Sutton, R. E. (2001) · Human Gene Therapy · doi:10.1089/104303401753153947 · Cited by 456 · see citing works
Inhibition of influenza virus production in virus-infected mice by RNA interference
Ge, Q., Filip, L., Bai, A., Nguyen, T., Eisen, H. N., & Chen, J. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402486101 · Cited by 444 · see citing works
The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro
Craigie, R., Fujiwara, T., & Bushman, F. (1990) · Cell · doi:10.1016/0092-8674(90)90126-y · Cited by 409 · see citing works
Role of the non‐homologous DNA end joining pathway in the early steps of retroviral infection
Li, L., Olvera, J. M., Yoder, K., Mitchell, R., Butler, S., Lieber, M., Martin, S. L., & Bushman, F. D. (2001) · EMBO Journal · doi:10.1093/emboj/20.12.3272 · Cited by 386 · see citing works
Nucleotide sequence and genomic organization of feline immunodeficiency virus.
Talbott, R. L., Sparger, E. E., Lovelace, K., Fitch, W. M., Pedersen, N. C., Luciw, P. A., & Elder, J. H. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.15.5743 · Cited by 383 · see citing works
Gene Therapy Progress and Prospects: Development of improved lentiviral and retroviral vectors – design, biosafety, and production
Sinn, P., Sauter, S., & McCray, P. B. (2005) · Gene Therapy · doi:10.1038/sj.gt.3302570 · Cited by 351 · see citing works
Self-citation
Identification of discrete functional domains of HIV‐1 integrase and their organization within an active multimeric complex.
Engelman, A., Bushman, F. D., & Craigie, R. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb05996.x · Cited by 350 · see citing works
The avian retroviral IN protein is both necessary and sufficient for integrative recombination in vitro
Katz, R. A., Merkel, G., Kulkosky, J., Leis, J., & Skalka, A. M. (1990) · Cell · doi:10.1016/0092-8674(90)90290-u · Cited by 346 · see citing works
Retroviral integrase domains: DNA binding and the recognition of LTR sequences
Khan, E., Mack, J. P. G., Katz, R. A., Kulkosky, J., & Skalka, A. M. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.4.851 · Cited by 287 · see citing works
HIV Integrase, a Brief Overview from Chemistry to Therapeutics*
Craigie, R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.r100027200 · Cited by 277 · see citing works
Human immunodeficiency virus type 1 integrase: effects of mutations on viral ability to integrate, direct viral gene expression from unintegrated viral DNA templates, and sustain viral propagation in primary cells
Wiskerchen, M. A., & Muesing, M. A. (1995) · Journal of Virology · doi:10.1128/jvi.69.1.376-386.1995 · Cited by 271 · see citing works
Lentiviral vectors with a defective integrase allow efficient and sustained transgene expression in vitro and in vivo
Philippe, S., Sarkis, C., Barkats, M., Mammeri, H., Ladroue, C., Petit, C., Mallet, J., & Serguera, C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0606197103 · Cited by 249 · see citing works
The core and carboxyl-terminal domains of the integrase protein of human immunodeficiency virus type 1 each contribute to nonspecific DNA binding
Engelman, A., Hickman, A. B., & Craigie, R. (1994) · Journal of Virology · doi:10.1128/jvi.68.9.5911-5917.1994 · Cited by 245 · see citing works
Human Immunodeficiency Virus cDNA Metabolism: Notable Stability of Two-Long Terminal Repeat Circles
Butler, S. L., Johnson, E. P., & Bushman, F. D. (2002) · Journal of Virology · doi:10.1128/jvi.76.8.3739-3747.2002 · Cited by 237 · see citing works
Silencing of episomal transgene expression by plasmid bacterial DNA elements in vivo
Chen, Z. Y., He, C. Y., Meuse, L. A., & Kay, M. A. (2004) · Gene Therapy · doi:10.1038/sj.gt.3302231 · Cited by 232 · see citing works
Human immunodeficiency virus type 1 integrase mutants retain in vitro integrase activity yet fail to integrate viral DNA efficiently during infection
Leavitt, A. D., Robles, G., Alesandro, N., & Varmus, H. E. (1996) · Journal of Virology · doi:10.1128/jvi.70.2.721-728.1996 · Cited by 232 · see citing works
Repair of gaps in retroviral DNA integration intermediates
Yoder, K. E., & Bushman, F. D. (2000) · Journal of Virology · doi:10.1128/jvi.74.23.11191-11200.2000 · Cited by 209 · see citing works
Integration is not necessary for expression of human immunodeficiency virus type 1 protein products
Stevenson, M., Haggerty, S., Lamonica, C. A., Meier, C. M., Welch, S. K., & Wasiak, A. (1990) · Journal of Virology · doi:10.1128/jvi.64.5.2421-2425.1990 · Cited by 206 · see citing works
Identification of amino acid residues critical for endonuclease and integration activities of HIV-1 IN protein in Vitro
Drelich, M., Wilhelm, R., & Mous, J. (1992) · Virology · doi:10.1016/0042-6822(92)90499-f · Cited by 206 · see citing works
Early steps of retrovirus replicative cycle
Nisole, S., & Saı̈b, A. (2004) · Retrovirology · doi:10.1186/1742-4690-1-9 · Cited by 202 · see citing works
Site-directed mutagenesis of HIV-1 integrase demonstrates differential effects on integrase functions in vitro.
Leavitt, A. D., Shiue, L., & VARMUS, H. E. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)53969-7 · Cited by 200 · see citing works
Transient Gene Expression by Nonintegrating Lentiviral Vectors
Nightingale, S. J., Hollis, R. P., Pepper, K. A., Petersen, D., Yu, X.-J., Yang, C., Bahner, I., & Kohn, D. B. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2006.01.008 · Cited by 192 · see citing works
Intrinsic Stability of Episomal Circles Formed during Human Immunodeficiency Virus Type 1 Replication
Pierson, T. C., Kieffer, T. L., Ruff, C. T., Buck, C. B., Gange, S. J., & Siliciano, R. F. (2002) · Journal of Virology · doi:10.1128/jvi.76.8.4138-4144.2002 · Cited by 178 · see citing works
Stable Gene Transfer to Muscle Using Non-integrating Lentiviral Vectors
Apolonia, L., Waddington, S. N., Fernandes, C., Ward, N. J., Bouma, G., Blundell, M. P., Thrasher, A. J., Collins, M. K., & Philpott, N. J. (2007) · Molecular Therapy · doi:10.1038/sj.mt.6300281 · Cited by 174 · see citing works
Therapeutic levels of human factor VIII and IX using HIV-1–based lentiviral vectors in mouse liver
Park, F., Ohashi, K., & Kay, M. A. (2000) · Blood · doi:10.1182/blood.v96.3.1173 · Cited by 157 · see citing works
Polynucleotidyl transfer reactions in transpositional DNA recombination.
Mizuuchi, K. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)36600-1 · Cited by 155 · see citing works
A stable complex between integrase and viral DNA ends mediates human immunodeficiency virus integration in vitro.
Ellison, V., & Brown, P. O. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.7316 · Cited by 154 · see citing works
Circularization of human immunodeficiency virus type 1 DNA in vitro
Farnet, C. M., & Haseltine, W. A. (1991) · Journal of Virology · doi:10.1128/jvi.65.12.6942-6952.1991 · Cited by 148 · see citing works
Novel Integrase-Defective Lentiviral Episomal Vectors for Gene Transfer
Vargas, J. J., Gusella, G., Najfeld, V., Klotman, M., & Cara, A. (2004) · Human Gene Therapy · doi:10.1089/104303404322959515 · Cited by 143 · see citing works
An Essential Interaction between Distinct Domains of HIV-1 Integrase Mediates Assembly of the Active Multimer
Ellison, V., Gerton, J. L., Vincent, K. A., & Brown, P. O. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.7.3320 · Cited by 131 · see citing works
Retroviral vectors: new applications for an old tool
Barquinero, J., Eixarch, H., & Pérez-Melgosa, M. (2004) · Gene Therapy · doi:10.1038/sj.gt.3302363 · Cited by 125 · see citing works
Structure-Based Mutational Analysis of the C-Terminal DNA-Binding Domain of Human Immunodeficiency Virus Type 1 Integrase: Critical Residues for Protein Oligomerization and DNA Binding
Lutzke, R. A. P., & Plasterk, R. H. A. (1998) · Journal of Virology · doi:10.1128/jvi.72.6.4841-4848.1998 · Cited by 124 · see citing works
HIV-1 gene expression: lessons from provirus and non-integrated DNA
Wu, Y. (2004) · Retrovirology · doi:10.1186/1742-4690-1-13 · Cited by 124 · see citing works
Intramolecular integration within Moloney murine leukemia virus DNA
Shoemaker, C. B., Hoffman, J., Goff, S. P., & Baltimore, D. (1981) · Journal of Virology · doi:10.1128/jvi.40.1.164-172.1981 · Cited by 121 · see citing works
Unintegrated Lentivirus DNA Persistence and Accessibility to Expression in Nondividing Cells: Analysis with Class I Integrase Mutants
Saenz, D. T., Loewen, N. A., Peretz, M., Whitwam, T., Barraza, R., Howell, K. G., Holmes, J. M., Good, M. S., & Poeschla, E. M. (2004) · Journal of Virology · doi:10.1128/jvi.78.6.2906-2920.2004 · Cited by 119 · see citing works
Gene Silencing by Small Regulatory RNAs in Mammalian Cells
Scherr, M., & Eder, M. (2007) · Cell Cycle · doi:10.4161/cc.6.4.3807 · Cited by 117 · see citing works
Erratum: Sustained correction of X-linked severe combined immunodeficiency by ex vivo gene therapy (New England Journal of Medicine (April 18, 2002) 346 (1185-1193))
Hacein‐Bey‐Abina, S., Deist, F. L., Carlier, F., Bouneaud, C., Hue, C., Villartay, J. D., Thrasher, A. J., Wulffraat, N. M., Sorensen, R. J. D., Dupuis‐Girod, S., et al. (2002) · UCL Discovery (University College London) · Cited by 117 · see citing works
CCR5: no longer a ‘good for nothing’ gene – chemokine control of West Nile virus infection
Lim, J. K., Glass, W. G., McDermott, D. H., & Murphy, P. M. (2006) · Trends in Immunology · doi:10.1016/j.it.2006.05.007 · Cited by 114 · see citing works
Human Immunodeficiency Virus Type 1 Replication in the Absence of Integrase-Mediated DNA Recombination: Definition of Permissive and Nonpermissive T-Cell Lines
Nakajima, N., Lu, R., & Engelman, A. (2001) · Journal of Virology · doi:10.1128/jvi.75.17.7944-7955.2001 · Cited by 113 · see citing works
Targeted Genome Modifications Using Integrase-deficient Lentiviral Vectors
Cornu, T. I., & Cathomen, T. (2007) · Molecular Therapy · doi:10.1038/sj.mt.6300345 · Cited by 105 · see citing works
Retrovirus Vectors: Toward the Plentivirus?
Baum, C., Schambach, A., Bohne, J., & Galla, M. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2006.03.007 · Cited by 103 · see citing works
Minicircle‐DNA production by site specific recombination and protein–DNA interaction chromatography
Mayrhofer, P., Blaesen, M., Schleef, M., & Jechlinger, W. (2008) · The Journal of Gene Medicine · doi:10.1002/jgm.1243 · Cited by 102 · see citing works
Efficient gene delivery to the adult and fetal CNS using pseudotyped non-integrating lentiviral vectors
Rahim, A. A., Wong, A. M. S., Howe, S. J., Buckley, S. M. K., Acosta-Saltos, A. D., Elston, K. E., Ward, N. J., Philpott, N. J., Cooper, J. D., Anderson, P. N., et al. (2009) · Gene Therapy · doi:10.1038/gt.2008.186 · Cited by 101 · see citing works
Purification of retroviral vectors for clinical application: Biological implications and technological challenges
Rodrigues, T., Carrondo, M. J. T., Alves, P. M., & Cruz, P. E. (2006) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2006.07.028 · Cited by 98 · see citing works
Human immunodeficiency virus type 1 preintegration complexes containing discontinuous plus strands are competent to integrate in vitro
Miller, M. D., Wang, B., & Bushman, F. D. (1995) · Journal of Virology · doi:10.1128/jvi.69.6.3938-3944.1995 · Cited by 96 · see citing works
Hybrid Lentivirus-transposon Vectors with a Random Integration Profile in Human Cells
Staunstrup, N. H., Moldt, B., Mátés, L., Villesen, P., Jakobsen, M., Ivics, Z., Izsvák, Z., & Mikkelsen, J. G. (2009) · Molecular Therapy · doi:10.1038/mt.2009.10 · Cited by 96 · see citing works
A Large U3 Deletion Causes Increased In Vivo Expression from a Nonintegrating Lentiviral Vector
Bayer, M., Kantor, B., Cockrell, A., Ma, H., Zeithaml, B., Li, X., McCown, T., & Kafri, T. (2008) · Molecular Therapy · doi:10.1038/mt.2008.199 · Cited by 94 · see citing works
Evidence for Gene Expression by Unintegrated Human Immunodeficiency Virus Type 1 DNA Species
Brussel, A., & Sonigo, P. (2004) · Journal of Virology · doi:10.1128/jvi.78.20.11263-11271.2004 · Cited by 93 · see citing works
Genetic Analyses of DNA-Binding Mutants in the Catalytic Core Domain of Human Immunodeficiency Virus Type 1 Integrase
Lu, R., Limón, A., Ghory, H. Z., & Engelman, A. (2005) · Journal of Virology · doi:10.1128/jvi.79.4.2493-2505.2005 · Cited by 92 · see citing works
Successful Immunization with a Single Injection of Non-integrating Lentiviral Vector
Negri, D. R., Michelini, Z., Baroncelli, S., Spada, M., Vendetti, S., Buffa, V., Bona, R., Leone, P., Klotman, M. E., & Cara, A. (2007) · Molecular Therapy · doi:10.1038/sj.mt.6300241 · Cited by 84 · see citing works
2-LTR Circular Viral DNA as a Marker for Human Immunodeficiency Virus Type 1 Infection in Vivo
Pauza, C. D., Trivedi, P., McKechnie, T. S., Richman, D. D., & Graziano, F. M. (1994) · Virology · doi:10.1006/viro.1994.1667 · Cited by 81 · see citing works
Expressing short hairpin RNAs in vivo
Snøve, O., & Rossi, J. (2006) · Nature Methods · doi:10.1038/nmeth927 · Cited by 76 · see citing works
Sleeping Beauty Transposition From Nonintegrating Lentivirus
Vink, C. A., Gaspar, H. B., Gabriel, R., Schmidt, M., McIvor, R. S., Thrasher, A. J., & Qasim, W. (2009) · Molecular Therapy · doi:10.1038/mt.2009.94 · Cited by 74 · see citing works
Central Core Domain of Retroviral Integrase Is Responsible for Target Site Selection
hio Shibagaki, Y., & Chow, S. A. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.13.8361 · Cited by 73 · see citing works
Protective Antiviral Immunity Conferred by a Nonintegrative Lentiviral Vector-Based Vaccine
Coutant, F., Frenkiel, M.-P., Despres, P., & Charneau, P. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003973 · Cited by 70 · see citing works
The MDS1–EVI1 Gene Complex as a Retrovirus Integration Site: Impact on Behavior of Hematopoietic Cells and Implications for Gene Therapy
Métais, J., & Dunbar, C. E. (2008) · Molecular Therapy · doi:10.1038/sj.mt.6300372 · Cited by 64 · see citing works
Rational Vector Design for Efficient Non-viral Gene Delivery: Challenges Facing the Use of Plasmid DNA
Mairhofer, J., & Grabherr, R. M. (2008) · Molecular Biotechnology · doi:10.1007/s12033-008-9046-7 · Cited by 63 · see citing works
Toolbox for retrovectorologists
Pagès, J., & Bru, T. (2004) · Journal of Gene Medicine · doi:10.1002/jgm.498 · Cited by 60 · see citing works
RNA Interference: A Tool for Querying Nervous System Function and an Emerging Therapy
Davidson, B. L., & Boudreau, R. L. (2007) · Neuron · doi:10.1016/j.neuron.2007.02.020 · Cited by 56 · see citing works
Integration Site Choice of a Feline Immunodeficiency Virus Vector
Kang, Y.-B., Moressi, C. J., Scheetz, T., Xie, L.-T., Tran, D., Casavant, T., Ak, P., Benham, C., Davidson, B., & McCray, P. (2006) · Journal of Virology · doi:10.1128/jvi.00719-06 · Cited by 53 · see citing works
Self-citation
Mutations in the Human Immunodeficiency Virus Type 1 Integrase D,D(35)E Motif Do Not Eliminate Provirus Formation
Gaur, M., & Leavitt, A. D. (1998) · Journal of Virology · doi:10.1128/jvi.72.6.4678-4685.1998 · Cited by 52 · see citing works
Characterization of Feline Immunodeficiency Virus Integrase and Analysis of Functional Domains
Shibagaki, Y., Holmes, M. L., Appa, R. S., & Chow, S. A. (1997) · Virology · doi:10.1006/viro.1997.8466 · Cited by 50 · see citing works
Identification of amino acids in HIV-2 integrase involved in site-specific hydrolysis and alcoholysis of viral DNA termini
van Gent, D. C., Groeneger, A. A. M. O., & Plasterk, R. H. A. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.15.3373 · Cited by 50 · see citing works
Genomic insertion of lentiviral DNA circles directed by the yeast Flp recombinase
Moldt, B., Staunstrup, N. H., Jakobsen, M., Yáñez-Muñoz, R. J., & Mikkelsen, J. G. (2008) · BMC Biotechnology · doi:10.1186/1472-6750-8-60 · Cited by 44 · see citing works
Comparison of wild‐type and class I integrase mutant‐FIV vectors in retina demonstrates sustained expression of integrated transgenes in retinal pigment epithelium
Loewen, N. A., Leske, D. A., Chen, Y., Teo, W., Saenz, D. T., Peretz, M., Holmes, J. M., & Poeschla, E. M. (2003) · The Journal of Gene Medicine · doi:10.1002/jgm.447 · Cited by 36 · see citing works
Targeted gene modification in mouse ES cells using integrase‐defective lentiviral vectors
Okada, Y., Ueshin, Y., Hasuwa, H., Takumi, K., Okabe, M., & Ikawa, M. (2009) · genesis · doi:10.1002/dvg.20469 · Cited by 23 · see citing works
Transient gene expression mediated by integrase-defective retroviral vectors.
Yu, S.-S., Dan, K., Chono, H., Chatani, E., Mineno, J., & Kato, I. (2008) · Biochemical and Biophysical Research Communications - BBRC · doi:10.1016/j.bbrc.2008.02.012 · Cited by 18 · see citing works
Conditionally replicating lentiviral-hybrid episomal vectors for suicide gene therapy.
Vargas, J. J., Klotman, M., & Cara, A. (2008) · Antiviral Research · doi:10.1016/j.antiviral.2008.06.015 · Cited by 17 · see citing works
Unintegrated viral DNA as a marker for human immunodeficiency virus 1 infection in vivo and in vitro.
Js, N. (1999) · PubMed · Cited by 3 · see citing works

Flagged first, then by citations

1 of the 87 references couldn’t be matched to a known paper.

Sorted by citations

Recent Advances in CRISPR/Cas9 Delivery Strategies
Yip, B. H. (2020) · Biomolecules · doi:10.3390/biom10060839 · Cited by 303 · see citing works
Gene therapy on the move
Kaufmann, K. B., Büning, H., Galy, A. H. M., Schambach, A., & Grez, M. (2013) · EMBO Molecular Medicine · doi:10.1002/emmm.201202287 · Cited by 282 · see citing works
Engineering mesenchymal stem cells for regenerative medicine and drug delivery
Park, J. S., Suryaprakash, S., Lao, Y., & Leong, K. W. (2015) · Methods · doi:10.1016/j.ymeth.2015.03.002 · Cited by 242 · see citing works
Improving the safety of cell therapy products by suicide gene transfer
Jones, B. S., Lamb, L. S., Goldman, F. D., & Stasi, A. D. (2014) · Frontiers in Pharmacology · doi:10.3389/fphar.2014.00254 · Cited by 201 · see citing works
The role of unintegrated DNA in HIV infection
Sloan, R. D., & Wainberg, M. A. (2011) · Retrovirology · doi:10.1186/1742-4690-8-52 · Cited by 155 · see citing works
Hepatocyte-targeted expression by integrase-defective lentiviral vectors induces antigen-specific tolerance in mice with low genotoxic risk
Mátrai, J., Cantore, A., Bartholomae, C. C., Annoni, A., Wang, W., Acosta-Sanchez, A., Samara-Kuko, E., De Waele, L., Ma, L., Genovese, P., et al. (2011) · Hepatology · doi:10.1002/hep.24230 · Cited by 139 · see citing works
Viral Vectors and Delivery Strategies for CNS Gene Therapy
Gray, S. J., Woodard, K., & Samulski, R. J. (2010) · Therapeutic Delivery · doi:10.4155/tde.10.50 · Cited by 124 · see citing works
Human Artificial Chromosomes for Gene Delivery and the Development of Animal Models
Kazuki, Y., & Oshimura, M. (2011) · Molecular Therapy · doi:10.1038/mt.2011.136 · Cited by 117 · see citing works
Recent progresses in gene delivery-based bone tissue engineering
Lu, C.-H., Chang, Y., Lin, S.-Y., Li, K., & Hu, Y. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.08.015 · Cited by 113 · see citing works
Assessment of F/HN-Pseudotyped Lentivirus as a Clinically Relevant Vector for Lung Gene Therapy
Uta Griesenbach, D., Inoue, M., Meng, C., Farley, R., Chan, M., Newman, N. K., Brum, A. M., You, J., Kerton, A., Shoemark, A., et al. (2012) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201206-1056oc · Cited by 108 · see citing works
Targeted immunotherapy of cancer with CAR T cells: achievements and challenges
Lipowska-Bhalla, G., Gilham, D., Hawkins, R., & Rothwell, D. (2012) · Cancer Immunology and Immunotherapy · doi:10.1007/s00262-012-1254-0 · Cited by 105 · see citing works
Laminopathies: The molecular background of the disease and the prospects for its treatment
Zaremba-Czogalla, M., Dubińska–Magiera, M., & Rzepecki, R. (2011) · Cellular & Molecular Biology Letters · doi:10.2478/s11658-010-0038-9 · Cited by 103 · see citing works
Development of hybrid viral vectors for gene therapy
Huang, S., & Kamihira, M. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.10.001 · Cited by 99 · see citing works
Pseudotype-Based Neutralization Assays for Influenza: A Systematic Analysis
Carnell, G., Ferrara, F., Grehan, K., Thompson, C., & Temperton, N. J. (2015) · Frontiers in Immunology · doi:10.3389/fimmu.2015.00161 · Cited by 93 · see citing works
Immunization Delivered by Lentiviral Vectors for Cancer and Infectious Diseases
Hu, B.-L., Tai, A., & Wang, P. (2011) · Immunological Reviews · doi:10.1111/j.1600-065x.2010.00967.x · Cited by 90 · see citing works
Neuron-Specific Gene Transfer Through Retrograde Transport of Lentiviral Vector Pseudotyped with a Novel Type of Fusion Envelope Glycoprotein
Kato, S., Kuramochi, M., Takasumi, K., Kobayashi, K., Inoue, K., Takahara, D., Hitoshi, S., Ikenaka, K., Shimada, T., Takada, M., et al. (2011) · Human Gene Therapy · doi:10.1089/hum.2011.111 · Cited by 78 · see citing works
A new generation of human artificial chromosomes for functional genomics and gene therapy
Kouprina, N., Earnshaw, W. C., Masumoto, H., & Larionov, V. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-1113-3 · Cited by 74 · see citing works
Non-Integrating Lentiviral Vectors in Clinical Applications: A Glance Through
Gurumoorthy, N., Nordin, F., Tye, G. J., Wan Kamarul Zaman, W. S., & Ng, M. H. (2022) · Biomedicines · doi:10.3390/biomedicines10010107 · Cited by 69 · see citing works
Insulin B chain 9–23 gene transfer to hepatocytes protects from type 1 diabetes by inducing Ag-specific FoxP3 + T regs
Akbarpour, M., Goudy, K., Cantore, A., Russo, F., Sanvito, F., Naldini, L., Annoni, A., & Roncarolo, M. G. (2015) · Science Translational Medicine · doi:10.1126/scitranslmed.aaa3032 · Cited by 66 · see citing works
Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice
Fujita, Y., Abe, R., Inokuma, D., Sasaki, M., Hoshina, D., Natsuga, K., Nishie, W., McMillan, J. R., Nakamura, H., Shimizu, T., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1000044107 · Cited by 64 · see citing works
Creating Higher Titer Lentivirus with Caffeine
Ellis, B. L., Potts, P. R., & Porteus, M. H. (2010) · Human Gene Therapy · doi:10.1089/hum.2010.068 · Cited by 62 · see citing works
Human artificial chromosome-based gene delivery vectors for biomedicine and biotechnology
Kouprina, N., Tomilin, A. N., Masumoto, H., Earnshaw, W. C., & Larionov, V. (2014) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2014.882314 · Cited by 60 · see citing works
Design and potential of non-integrating lentiviral vectors
Shaw, A., & Cornetta, K. (2014) · Biomedicines · doi:10.3390/biomedicines2010014 · Cited by 60 · see citing works
LDLR-Gene therapy for familial hypercholesterolaemia: problems, progress, and perspectives
Al-Allaf, F., Coutelle, C., Waddington, S., David, A., Harbottle, R., & Themis, M. (2010) · International Archives of Medicine · doi:10.1186/1755-7682-3-36 · Cited by 63 · see citing works
Histone Deacetylase Inhibition Activates Transgene Expression from Integration-Defective Lentiviral Vectors in Dividing and Non-Dividing Cells
Pelascini, L. P. L., Janssen, J. M., & Gonçalves, M. A. F. V. (2012) · Human Gene Therapy · doi:10.1089/hum.2012.069 · Cited by 57 · see citing works
CCR5 Gene Editing of Resting CD4+ T Cells by Transient ZFN Expression From HIV Envelope Pseudotyped Nonintegrating Lentivirus Confers HIV-1 Resistance in Humanized Mice
Yi, G., Choi, J. G., Bharaj, P., Abraham, S., Dang, Y., Kafri, T., Alozie, O. K., Manjunath, M. N., & Shankar, P. (2014) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2014.52 · Cited by 52 · see citing works
Engineering of α-PD-1 antibody-expressing long-lived plasma cells by CRISPR/Cas9-mediated targeted gene integration
Luo, B., Zhan, Y., Luo, M., Dong, H., Liu, J., Lin, Y., Zhang, J., Wang, G., Verhoeyen, E., Zhang, Y., et al. (2020) · Cell Death and Disease · doi:10.1038/s41419-020-03187-1 · Cited by 49 · see citing works
Histones Are Rapidly Loaded onto Unintegrated Retroviral DNAs Soon after Nuclear Entry
Wang, G. Z., Wang, Y., & Goff, S. P. (2016) · Cell Host & Microbe · doi:10.1016/j.chom.2016.10.009 · Cited by 48 · see citing works
Integration-deficient Lentiviral Vectors Expressing Codon-optimized R338L Human FIX Restore Normal Hemostasis in Hemophilia B Mice
Suwanmanee, T., Hu, G., Gui, T., Bartholomae, C. C., Kutschera, I., Kalle, C. V., Schmidt, M., Monahan, P. E., & Kafri, T. (2013) · Molecular Therapy · doi:10.1038/mt.2013.188 · Cited by 44 · see citing works
CHM/REP1 cDNA delivery by lentiviral vectors provides functional expression of the transgene in the retinal pigment epithelium of choroideremia mice
Tolmachova, T., Tolmachov, O. E., Wavre‐Shapton, S. T., Tracey‐White, D., Futter, C. E., & Seabra, M. C. (2012) · The Journal of Gene Medicine · doi:10.1002/jgm.1652 · Cited by 45 · see citing works
Optimization of Lentiviral Vectors Generation for Biomedical and Clinical Research Purposes: Contemporary Trends in Technology Development and Applications
Kumar, P., & Chan, W. (2011) · Current Gene Therapy · doi:10.2174/156652311794940782 · Cited by 40 · see citing works
LV305, a dendritic cell-targeting integration-deficient ZVex TM -based lentiviral vector encoding NY-ESO-1, induces potent anti-tumor immune response
Albershardt, T. C., Campbell, D. J., Parsons, A. J., Slough, M. M., ter Meulen, J., & Berglund, P. (2016) · Molecular Therapy - Oncolytics · doi:10.1038/mto.2016.10 · Cited by 38 · see citing works
S/MAR sequence confers long-term mitotic stability on non-integrating lentiviral vector episomes without selection
Verghese, S. C., Goloviznina, N. A., Skinner, A. M., Lipps, H. J., & Kurre, P. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku082 · Cited by 37 · see citing works
Sweat gland regeneration: Current strategies and future opportunities.
Chen, R.-K., Zhu, Z., Ji, S.-F., Geng, Z.-J., Hou, Q., Sun, X.-Y., & Fu, X.-B. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120201 · Cited by 34 · see citing works
A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function
Kononenko, A. V., Bansal, R., Lee, N., Grimes, B. R., Masumoto, H., Earnshaw, W. C., Larionov, V., & Kouprina, N. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku870 · Cited by 36 · see citing works
Homology-directed gene-editing approaches for hematopoietic stem and progenitor cell gene therapy
Azhagiri, M. K. K., Babu, P., Venkatesan, V., & Thangavel, S. (2021) · Stem Cell Research & Therapy · doi:10.1186/s13287-021-02565-6 · Cited by 35 · see citing works
Optimizing cancer treatment: the synergistic potential of CAR-T cell therapy and CRISPR/Cas9
Amiri, M., Moaveni, A. K., Zolbin, M. M., Shademan, B., & Nourazarian, A. (2024) · Frontiers in Immunology · doi:10.3389/fimmu.2024.1462697 · Cited by 35 · see citing works
Transduction of Human Antigen-Presenting Cells with Integrase-Defective Lentiviral Vector Enables Functional Expansion of Primed Antigen-Specific CD8 + T Cells
Negri, D. R. M., Bona, R., Michelini, Z., Leone, P., Macchia, I., Klotman, M. E., Salvatore, M., & Cara, A. (2010) · Human Gene Therapy · doi:10.1089/hum.2009.200 · Cited by 34 · see citing works
Use of lentiviral vectors in vaccination
Ku, M.-W., Charneau, P., & Majlessi, L. (2021) · Expert Review of Vaccines · doi:10.1080/14760584.2021.1988854 · Cited by 35 · see citing works
MuLV IN mutants responsive to HDAC inhibitors enhance transcription from unintegrated retroviral DNA
Schneider, W. M., Wu, D., Amin, V., Aiyer, S., & Roth, M. J. (2012) · Virology · doi:10.1016/j.virol.2012.01.034 · Cited by 32 · see citing works
In search of an ideal template for therapeutic genome editing: A review of current developments for structure optimization
Shakirova, A. I., Karpov, T. E., Komarova, Y., & Lepik, K. V. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1068637 · Cited by 33 · see citing works
HIV-derived vectors for therapy and vaccination against HIV
Nunzio, F. D., Félix, T., Arhel, N. J., Nisole, S., Charneau, P., & Beignon, A. (2012) · Vaccine · doi:10.1016/j.vaccine.2012.01.089 · Cited by 32 · see citing works
Transcription factor induced conversion of human fibroblasts towards the hair cell lineage
Alonso, M. B. D., López‐Hernández, I., de la Fuente, M. Á., Garcı́a-Sancho, J., Giráldez, F., & Schimmang, T. (2018) · PLoS ONE · doi:10.1371/journal.pone.0200210 · Cited by 30 · see citing works
Gene Editing for the Treatment of Primary Immunodeficiency Diseases
Rai, R., Thrasher, A. J., & Cavazza, A. (2020) · Human Gene Therapy · doi:10.1089/hum.2020.185 · Cited by 30 · see citing works
Immunomodulatory effects of stem cells: Therapeutic option for neurodegenerative disorders
Čaprnda, M., Kubatka, P., Gazdíková, K., Gašparová, I., Valentová, V., Stollárová, N., Rocca, G. L., Kobyliak, N. M., Dragašek, J., Mozos, I. M., et al. (2017) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2017.04.034 · Cited by 31 · see citing works
Stable expression of large transgenes via the knock-in of an integrase-deficient lentivirus
Chavez, M., Rane, D. A., Chen, X., & Qi, L. S. (2023) · Nature Biomedical Engineering · doi:10.1038/s41551-023-01037-x · Cited by 30 · see citing works
Zinc finger nucleases: Tailor-made for gene therapy
Chou, S.-T., Leng, Q., & Mixson, A. J. (2012) · Drugs of the Future · doi:10.1358/dof.2012.37.3.1779022 · Cited by 29 · see citing works
The HIV-1 Rev/RRE system is required for HIV-1 5' UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particles
Cockrell, A. S., van Praag, H., Santistevan, N. J., Ma, H., & Kafri, T. (2011) · Retrovirology · doi:10.1186/1742-4690-8-51 · Cited by 29 · see citing works
Respiratory Syncytial Virus Engineered To Express the Cystic Fibrosis Transmembrane Conductance Regulator Corrects the Bioelectric Phenotype of Human Cystic Fibrosis Airway Epithelium In Vitro
Kwilas, A. R., Yednak, M. A., Zhang, L., Liesman, R. M., Collins, P. L., Pickles, R. J., & Peeples, M. E. (2010) · Journal of Virology · doi:10.1128/jvi.00346-10 · Cited by 29 · see citing works
Lentiviral Vectors as a Vaccine Platform against Infectious Diseases
Nemirov, K., Bourgine, M., Anna, F., Yu, W., Charneau, P., & Majlessi, L. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15030846 · Cited by 28 · see citing works
Prototype Foamy Virus Protease Activity Is Essential for Intraparticle Reverse Transcription Initiation but Not Absolutely Required for Uncoating upon Host Cell Entry
Hütter, S., Müllers, E., Stanke, N., Reh, J., & Lindemann, D. (2013) · Journal of Virology · doi:10.1128/jvi.02323-12 · Cited by 28 · see citing works
Lentiviral Protein Transfer Vectors Are an Efficient Vaccine Platform and Induce a Strong Antigen-Specific Cytotoxic T Cell Response
Uhlig, K. M., Schülke, S., Scheuplein, V., Malczyk, A. H., Reusch, J., Kugelmann, S., Muth, A., Koch, V., Hutzler, S., Bodmer, B. S., et al. (2015) · Journal of Virology · doi:10.1128/jvi.00844-15 · Cited by 27 · see citing works
Efficient Transient Genetic Manipulation In Vitro and In Vivo by Prototype Foamy Virus-mediated Nonviral RNA Transfer
Hamann, M. V., Stanke, N., Müllers, E., Stirnnagel, K., Hütter, S., Artegiani, B., Alonso, S. B., Calegari, F., & Lindemann, D. (2014) · Molecular Therapy · doi:10.1038/mt.2014.82 · Cited by 27 · see citing works
Viral-mediated overexpression of mutant huntingtin to model HD in various species
Ruiz, M., & Déglon, N. (2011) · Neurobiology of Disease · doi:10.1016/j.nbd.2011.08.023 · Cited by 27 · see citing works
Integration-deficient lentivectors: an effective strategy to purify and differentiate human embryonic stem cell-derived hepatic progenitors
Yang, G., Si-Tayeb, K., Corbineau, S., Vernet, R., Gayon, R., Dianat, N., Martinet, C., Clay, D., Goulinet-Mainot, S., Tachdjian, G., et al. (2013) · BMC Biology · doi:10.1186/1741-7007-11-86 · Cited by 26 · see citing works
Exploring Advanced CRISPR Delivery Technologies for Therapeutic Genome Editing
Rostami, N., Gomari, M. M., Choupani, E., Abkhiz, S., Fadaie, M., Eslami, S. S., Mahmoudi, Z., Zhang, Y., Puri, M., Monfared, F. N., et al. (2024) · Small Science · doi:10.1002/smsc.202400192 · Cited by 26 · see citing works
Gene delivery in tissue engineering and regenerative medicine
Fang, Y. L., Chen, X., & Godbey, W. T. (2014) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33354 · Cited by 24 · see citing works
Nonintegrating Lentiviral Vector-Based Vaccine Efficiently Induces Functional and Persistent CD8+ T Cell Responses in Mice
Negri, D. R. M., Michelini, Z., Baroncelli, S., Spada, M., Vendetti, S., Bona, R., Leone, P., Klotman, M. E., & Cara, A. (2010) · BioMed Research International · doi:10.1155/2010/534501 · Cited by 24 · see citing works
Recent Advances in Lentiviral Vaccines for HIV-1 Infection
Norton, T. D., & Miller, E. A. (2016) · Frontiers in Immunology · doi:10.3389/fimmu.2016.00243 · Cited by 23 · see citing works
Transgenic Expression of Human Glial Cell Line-Derived Neurotrophic Factor from Integration-Deficient Lentiviral Vectors is Neuroprotective in a Rodent Model of Parkinson's Disease
Lu-Nguyen, N. B., Broadstock, M., Schliesser, M. G., Bartholomae, C., von Kalle, C., Schmidt, M., & Yáñez-Muñoz, R. (2014) · Human Gene Therapy · doi:10.1089/hum.2014.003 · Cited by 22 · see citing works
Simian immunodeficiency virus-Vpx for improving integrase defective lentiviral vector-based vaccines
Negri, D. R. M., Rossi, A., Blasi, M., Michelini, Z., Leone, P., Chiantore, M. V., Baroncelli, S., Perretta, G., Cimarelli, A., Klotman, M. E., et al. (2012) · Retrovirology · doi:10.1186/1742-4690-9-69 · Cited by 22 · see citing works
Paralleled comparison of vectors for the generation of CAR-T cells
Qin, D., Huang, Y., Li, D., Wang, Y., Wang, W., & Wei, Y.-Q. (2016) · Anti-Cancer Drugs · doi:10.1097/cad.0000000000000387 · Cited by 21 · see citing works
CRISPR/Cas9 technology: towards a new generation of improved CAR-T cells for anticancer therapies
Ureña-Bailén, G., Lamsfus‐Calle, A., Daniel-Moreno, A., Raju, J., Schlegel, P., Seitz, C. M., Atar, D., Antony, J. S., Handgretinger, R., & Mezger, M. (2019) · Briefings in Functional Genomics · doi:10.1093/bfgp/elz039 · Cited by 23 · see citing works
Integrase-defective lentiviral vectors encoding cytokines induce differentiation of human dendritic cells and stimulate multivalent immune responses in vitro and in vivo
Daenthanasanmak, A., Salguero, G., Borchers, S., Figueiredo, C., Jacobs, R., Sundarasetty, B. S., Schneider, A., Schambach, A., Eiz‐Vesper, B., Blasczyk, R., et al. (2012) · Vaccine · doi:10.1016/j.vaccine.2012.05.063 · Cited by 20 · see citing works
Genome Editing for Human Gene Therapy
Meissner, T. B., Mandal, P. K., Ferreira, L. M. R., Rossi, D. J., & Cowan, C. A. (2014) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-801185-0.00013-1 · Cited by 20 · see citing works
Non-integrating lentiviral vectors based on the minimal S/MAR sequence retain transgene expression in dividing cells
Xu, Z., Chen, F., Zhang, L., Lu, J., Xu, P., Liu, G., Xie, X., Mu, W., Wang, Y., & Liu, D. (2016) · Science China Life Sciences · doi:10.1007/s11427-016-0067-0 · Cited by 20 · see citing works
HIV-1 Conserved Mosaics Delivered by Regimens with Integration-Deficient DC-Targeting Lentiviral Vector Induce Robust T Cells
Wee, E. G., Ondondo, B., Berglund, P., Archer, J. F., McMichael, A. J., Baltimore, D., ter Meulen, J. H., & Hanke, T. (2017) · Molecular Therapy · doi:10.1016/j.ymthe.2016.12.004 · Cited by 20 · see citing works
Zinc finger nucleases: Tailor-made for gene therapy
Chou, S., Leng, Q., & Mixson, A. J. (2012) · Drugs of the Future · doi:10.1358/dof.2012.037.03.1779022 · Cited by 39 · see citing works
Perinatal Gene Transfer to the Liver
McKay, T. R., Rahim, A. A., Buckley, S. M. K., Ward, N. J., Chan, J. K. Y., Howe, S. J., & Waddington, S. N. (2011) · Current Pharmaceutical Design · doi:10.2174/138161211797247541 · Cited by 20 · see citing works
Advances in HIV Gene Therapy
Kitawi, R. C., Ledger, S., Kelleher, A. D., & Ahlenstiel, C. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25052771 · Cited by 18 · see citing works
Emerging trends in virus and virus-like particle gene therapy delivery to the brain
Mandalawatta, H. P. P., Rajendra, K. C., Fairfax, K. A., & Hewitt, A. W. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102280 · Cited by 18 · see citing works
Mucosal Immunization with Integrase-Defective Lentiviral Vectors Protects against Influenza Virus Challenge in Mice
Fontana, J. M., Christos, P. J., Michelini, Z., Negri, D. R. M., Cara, A., & Salvatore, M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0097270 · Cited by 18 · see citing works
Integrase deficient lentiviral vector: prospects for safe clinical applications
Yew, C.-H. T., Gurumoorthy, N., Nordin, F., Tye, G. J., Zaman, W. S. W. K., Tan, J. J., & Ng, M. H. (2022) · PeerJ · doi:10.7717/peerj.13704 · Cited by 17 · see citing works
Homology-Directed-Repair-Based Genome Editing in HSPCs for the Treatment of Inborn Errors of Immunity and Blood Disorders
Allen, D. G., Kalter, N., Rosenberg, M., & Hendel, A. (2023) · Pharmaceutics · doi:10.3390/pharmaceutics15051329 · Cited by 17 · see citing works
Non-Integrative Lentivirus Drives High-Frequency cre-Mediated Cassette Exchange in Human Cells
Torres-Ruiz, R., García, A., Payá, M., & Ramı́rez, J. C. (2011) · PLoS ONE · doi:10.1371/journal.pone.0019794 · Cited by 17 · see citing works
Hybrid Lentiviral Vectors
Qasim, W., Vink, C. A., & Thrasher, A. J. (2010) · Molecular Therapy · doi:10.1038/mt.2010.76 · Cited by 16 · see citing works
A Curative DNA Code for Hematopoietic Defects
Porteus, M. H., Pavel-Dinu, M., & Pai, S. (2022) · Hematology/Oncology Clinics of North America · doi:10.1016/j.hoc.2022.05.002 · Cited by 15 · see citing works
Using Lentiviral Vectors as Delivery Vehicles for Gene Therapy
Dissen, G. A., McBride, J. L., Lomniczi, A., Matagne, V., Dorfman, M. D., Neff, T. L., Galimi, F., & Ojeda, S. R. (2011) · Neuromethods · doi:10.1007/978-1-61779-533-6_4 · Cited by 15 · see citing works
Generation of a stable packaging cell line producing high-titer PPT-deleted integration-deficient lentiviral vectors
Hu, P., Li, Y., Sands, M. S., McCown, T. J., & Kafri, T. (2015) · Molecular Therapy — Methods & Clinical Development · doi:10.1038/mtm.2015.25 · Cited by 15 · see citing works
Improved retroviral episome transfer of transcription factors enables sustained cell fate modification
Schott, J., Hoffmann, D., Maetzig, T., Müller, F.-J., Steinemann, D., Zychlinski, D., Cantz, T., Baum, C., & Schambach, A. (2014) · Gene Therapy · doi:10.1038/gt.2014.69 · Cited by 13 · see citing works
One-year follow-up of transgene expression by integrase-defective lentiviral vectors and their therapeutic potential in spinocerebellar ataxia model mice
Saida, H., Matsuzaki, Y., Takayama, K., Iizuka, A., Konno, A., Yanagi, S., & Hirai, H. (2014) · Gene Therapy · doi:10.1038/gt.2014.60 · Cited by 11 · see citing works
Retrovirus-based vectors for transient and permanent cell modification
Schott, J. W., Hoffmann, D., & Schambach, A. (2015) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2015.09.004 · Cited by 11 · see citing works
Adeno-associated virus Rep-mediated targeting of integrase-defective retroviral vector DNA circles into human chromosome 19
Huang, S., Kawabe, Y., Itô, A., & Kamihira, M. (2011) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2011.11.059 · Cited by 11 · see citing works
Homologous recombination‐based gene therapy for the primary immunodeficiencies
Porteus, M. H. (2011) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2011.06314.x · Cited by 10 · see citing works
3′ Self-Inactivating Long Terminal Repeat Inserts for the Modulation of Transgene Expression from Lentiviral Vectors
Manic, G., Maurin-Marlin, A., Galluzzi, L., Subra, F., Mouscadet, J. F., & Bury‐Moné, S. (2012) · Human Gene Therapy Methods · doi:10.1089/hgtb.2011.154 · Cited by 10 · see citing works
Hutchinson Gilford Progeria Syndrome: A Therapeutic Approach via Adenoviral Delivery of CRISPR/cas Genome Editing System
Arancio, W. (2015) · Journal of Genetic Syndromes & Gene Therapy · doi:10.4172/2157-7412.1000256 · Cited by 9 · see citing works
A novel dendritic cell-targeted lentiviral vector, encoding Ag85A-ESAT6 fusion gene of Mycobacterium tuberculosis, could elicit potent cell-mediated immune responses in mice
Shakouri, M., Moazzeni, S. M., Ghanei, M., Arashkia, A., Etemadzadeh, M. H., & Azadmanesh, K. (2016) · Molecular Immunology · doi:10.1016/j.molimm.2016.04.014 · Cited by 9 · see citing works
Episomal lentiviral vectors confer erythropoietin expression in dividing cells
Chen, F., Qi, X., Zhang, R., Wu, Z., Yan, C., Li, J., Liu, Q., & Qi, J. (2017) · Plasmid · doi:10.1016/j.plasmid.2017.02.001 · Cited by 9 · see citing works
P95 Assessment of F/HN-pseudotyped Lentivirus as a Clinically Relevant Vector For Lung Gene Therapy
Uta Griesenbach, D., Inoue, M., Meng, C., Farley, R., Chan, M., Newman, N., Brum, A. M., You, J., Kerton, A., Shoemark, A., et al. (2012) · Thorax · doi:10.1136/thoraxjnl-2012-202678.337 · Cited by 8 · see citing works
Mutagenesis and Carcinogenesis Risk Evaluation for AAV and Lentiviral Gene Therapies
Parman, T., Pizzurro, D. M., Lucas, J., & Peng, Z. (2025) · International Journal of Toxicology · doi:10.1177/10915818251318248 · Cited by 7 · see citing works
Suppressing the malignant phenotypes of glioma cells by lentiviral delivery of small hairpin RNA targeting hypoxia-inducible factor-1α.
Dong, C., Wu, W. K. K., Feng, S., Yu, J., Shao, J. F., & He, G. M. (2013) · Europe PMC (PubMed Central) · Cited by 7 · see citing works
Initial Characterization of Integrase-Defective Lentiviral Vectors for Pancreatic Cancer Gene Therapy
Hanoun, N., Gayral, M., Pointreau, A., Buscail, L., & Cordelier, P. (2016) · Human Gene Therapy · doi:10.1089/hum.2015.151 · Cited by 7 · see citing works
Genome Engineering for Therapeutic Applications
Thakore, P. I., & Gersbach, C. A. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-800563-7.00003-8 · Cited by 7 · see citing works
Designing nucleic acid-based therapeutics for cancer treatment: Updates on the state of the art
Peng, B., Jayasinghe, M. K., Le, A. H., Tran, N. M., & Le, M. T. N. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.09.041 · Cited by 6 · see citing works
Establishment and evaluation of a murine αvβ3-integrin-expressing cell line with increased susceptibility to Foot-and-mouth disease virus
Zhang, W., Lian, K., Yang, F., Yang, Y., Zhu, Z., Zhu, Z., Cao, W., Mao, R., Ye, J., He, J., et al. (2015) · Journal of Veterinary Science · doi:10.4142/jvs.2015.16.3.265 · Cited by 6 · see citing works
HIV-Derived Vectors for Gene Therapy Targeting Dendritic Cells
Rossetti, M. M., Cavarelli, M., Gregori, S., & Scarlatti, G. (2012) · Advances in experimental medicine and biology · doi:10.1007/978-1-4614-4433-6_9 · Cited by 6 · see citing works
A p21 reporter iPSC line for evaluating CRISPR-Cas9 and vector-induced stress responses
Sun, Y.-D., Li, G., Zhang, F., Cheng, T., Zhang, J., & Zhang, X. (2024) · Stem Cells · doi:10.1093/stmcls/sxae056 · Cited by 5 · see citing works
Deciphering the impact of parameters influencing transgene expression kinetics after repeated cell transduction with integration‐deficient retroviral vectors
Schott, J. W., Jäschke, N. M., Hoffmann, D., Maetzig, T., Ballmaier, M., Godinho, T., Cathomen, T., & Schambach, A. (2015) · Cytometry Part A · doi:10.1002/cyto.a.22650 · Cited by 5 · see citing works
Enhancement of mouse hematopoietic stem/progenitor cell function via transient gene delivery using integration-deficient lentiviral vectors
Alonso-Ferrero, M. E., van Til, N. P., Bartolović, K., Mata, M. F., Wagemaker, G., MOULDING, D. A., Williams, D. A., Kinnon, C., Waddington, S. N., Milsom, M. D., et al. (2017) · Experimental Hematology · doi:10.1016/j.exphem.2017.09.003 · Cited by 5 · see citing works
Preparation and characterization of a new monoclonal antibody against CXCR4 using lentivirus vector
Li, X., Kuang, Y., Huang, X., Zou, L., Huang, L., Yang, T., Li, W., & Yang, Y. (2016) · International Immunopharmacology · doi:10.1016/j.intimp.2016.04.020 · Cited by 4 · see citing works

Non-Integrating Lentiviral Vectors
Sarkis, C., Philippe, S., Mallet, J. J., & Serguera, C. (2008) · Current Gene Therapy · doi:10.2174/156652308786848012 · Cited by 74 · see citing works
Lentiviral vectors with a defective integrase allow efficient and sustained transgene expression in vitro and in vivo
Philippe, S., Sarkis, C., Barkats, M., Mammeri, H., Ladroue, C., Petit, C., Mallet, J., & Serguera, C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0606197103 · Cited by 249 · see citing works
Design and potential of non-integrating lentiviral vectors
Shaw, A., & Cornetta, K. (2014) · Biomedicines · doi:10.3390/biomedicines2010014 · Cited by 60 · see citing works
Use of integrase-minus lentiviral vector for transient expression.
Farazmandfar, T., Shahreza, H. K., Haghshenas, M. R., Janbabai, G., Azadeh, H., & Samaei, N. M. (2012) · Europe PMC (PubMed Central) · Cited by 10 · see citing works
Integration-deficient Lentiviral Vectors: A Slow Coming of Age
Wanisch, K., & Yáñez-Muñoz, R. J. (2009) · Molecular Therapy · doi:10.1038/mt.2009.122 · Cited by 222 · see citing works

Authors: Banasik, M. B., & McCray, P. B.
Affiliations: University of Iowa (US)
Corresponding author: McCray, P B
ORCID: McCray, P B
Venue: Gene Therapy
Publisher: Springer Science and Business Media LLC
Published: 2009-10-22
Volume: 17
Issue: 2
Pages: 150-157
ISSN: 0969-7128 (ISSN-L)
1476-5462
DOI: 10.1038/gt.2009.135
OpenAlex: W1964810194
Cited by: 129
References: 87
Citations by year: 2026: 1 · 2025: 3 · 2024: 7 · 2023: 6 · 2022: 5 · 2021: 4 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 5 · 2016: 10 · 2015: 10 · 2014: 16 · 2013: 8 · 2012: 17
FWCI: 1.39
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Gene Therapy (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords: lentiviral vectors, non-integrating vectors, insertional mutagenesis, transient expression, pseudotyping, cell tropism, gene therapy vectors
MeSH terms: Animals, Genetic Vectors, Genetic Therapy, Gene Expression, Lentivirus, Mutagenesis, Insertional, Virus Integration, Gene Transfer Techniques, Transgenes, Integrases

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Integrase-defective lentiviral vectors: Progress and applications

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Cites

Cited by

Related

Record

About these checks