Cited by 37Hematopoietic Stem Cell TransplantationEffects of Radiation ExposureRedox biology and oxidative stress

Hematopoietic stem cell regeneration enhanced by ectopic expression of ROS-detoxifying enzymes in transplant mice.

Open access
Miao, Weimin, XuFeng, Richard, Park, Moo-Rim, Gu, Haihui, Hu, Linping, Kang, Jin Wook, Ma, Shihui, Liang, Paulina H +6 more·2013·Molecular Therapy

Abstract

High levels of reactive oxygen species (ROS) can exhaust hematopoietic stem cells (HSCs). Thus, maintaining a low state of redox in HSCs by modulating ROS-detoxifying enzymes may augment the regeneration potential of HSCs. Our results show that basal expression of manganese superoxide dismutase (MnSOD) and catalase were at low levels in long-term and short-term repopulating HSCs, and administration of a MnSOD plasmid and lipofectin complex (MnSOD-PL) conferred radiation protection on irradiated recipient mice. To assess the intrinsic role of elevated MnSOD or catalase in HSCs and hematopoietic progenitor cells, the MnSOD or catalase gene was overexpressed in mouse hematopoietic cells via retroviral transduction. The impact of MnSOD and catalase on hematopoietic progenitor cells was mild, as measured by colony-forming units (CFUs). However, overexpressed catalase had a significant beneficial effect on long-term engraftment of transplanted HSCs, and this effect was further enhanced after an insult of low-dose γ-irradiation in the transplant mice. In contrast, overexpressed MnSOD exhibited an insignificant effect on long-term engraftment of transplanted HSCs, but had a significant beneficial effect after an insult of sublethal irradiation. Taken together, these results demonstrate that HSC function can be enhanced by ectopic expression of ROS-detoxifying enzymes, especially after radiation exposure in vivo.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/mt.2012.232

Cites

44 of 44 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Miao, W., XuFeng, R., Park, M.-R., Gu, H., Hu, L., Kang, J. W., Ma, S., Liang, P. H., Li, Y., Cheng, H., Yu, H., Epperly, M., Greenberger, J., & Cheng, T.
Affiliations
  • Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
  • University of Pittsburgh (US)
Corresponding author
Cheng, Tao
Venue
Molecular Therapy
Publisher
Elsevier BV
Published
2013-02
Volume
21
Issue
2
Pages
423-432
ISSN
1525-0016 (ISSN-L)
OpenAlex
W2089961057
Cited by
37
References
44
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 4 · 2017: 5 · 2016: 2 · 2015: 5 · 2014: 5 · 2013: 1 · 2012: 1
FWCI
3.11
Type
journal article
Language
en
Open access
Locations
  • Molecular Therapy (publishedVersion, cc-by-nc-nd)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (R01-AI080424)
  • Leukemia and Lymphoma Society (1027-08, 30825017, U19AI68021)
  • National Natural Science Foundation of China (81090410, 90913018)
  • Ministry of Science and Technology of the People's Republic of China (2010DFB30270, 2011CB964801, 2011ZX09102-010)
Topics
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
hematopoietic stem cells, reactive oxygen species, manganese superoxide dismutase, catalase, reactive oxygen species scavenging, long-term engraftment, hematopoietic progenitor cells, gamma irradiation, radiation protection, retroviral gene transfer
MeSH terms
Animals, Catalase, Female, Flow Cytometry, Gene Expression Regulation, Genetic Vectors, Hematopoietic Stem Cells, Humans, Mice, Inbred C57BL, Oxidation-Reduction, Retroviridae, Stem Cells, Superoxide Dismutase, Transduction, Genetic, Blotting, Western, Reactive Oxygen Species, Hematopoietic Stem Cell Transplantation, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review