The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
The vacuolar Ca2+-activated channel TPC1 regulates germination and stomatal movement
Abstract
Cytosolic free calcium ([Ca2+]cyt) is a ubiquitous signalling component in plant cells. Numerous stimuli trigger sustained or transient elevations of [Ca2+]cyt that evoke downstream stimulus-specific responses. Generation of [Ca2+]cyt signals is effected through stimulus-induced opening of Ca2+-permeable ion channels that catalyse a flux of Ca2+ into the cytosol from extracellular or intracellular stores. Many classes of Ca2+ current have been characterized electrophysiologically in plant membranes. However, the identity of the ion channels that underlie these currents has until now remained obscure. Here we show that the TPC1 (‘two-pore channel 1’) gene of Arabidopsis thaliana encodes a class of Ca2+-dependent Ca2+-release channel that is known from numerous electrophysiological studies as the slow vacuolar channel. Slow vacuolar channels are ubiquitous in plant vacuoles, where they form the dominant conductance at micromolar [Ca2+]cyt. We show that a tpc1 knockout mutant lacks functional slow vacuolar channel activity and is defective in both abscisic acid-induced repression of germination and in the response of stomata to extracellular calcium. These studies unequivocally demonstrate a critical role of intracellular Ca2+-release channels in the physiological processes of plants.
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Cites
Floral dip: a simplified method forAgrobacterium‐mediated transformation ofArabidopsis thaliana
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Self-citationAbscisic Acid Signaling in Seeds and Seedlings
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Calcium at the Crossroads of Signaling
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Self-citationG UARD C ELL S IGNAL T RANSDUCTION
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Cited by
White, P. J., & Broadley, M. R. (2009) · New Phytologist · doi:10.1111/j.1469-8137.2008.02738.x · Cited by 2,178
The Language of Calcium Signaling
Dodd, A. N., Kudla, J., & Sanders, D. (2010) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-070109-104628 · Cited by 1,317
Self-citationNAADP mobilizes calcium from acidic organelles through two-pore channels
Calcraft, P. J., Ruas, M., Pan, Z., Cheng, X., Arredouani, A., Hao, X., Tang, J., Rietdorf, K., Teboul, L., Chuang, K.-T., et al. (2009) · Nature · doi:10.1038/nature08030 · Cited by 760
Calcium transport across plant membranes: mechanisms and functions
Demidchik, V., Shabala, S., Isayenkov, S., Cuin, T. A., & Pottosin, I. (2018) · New Phytologist · doi:10.1111/nph.15266 · Cited by 459
Closing gaps: linking elements that control stomatal movement
Kollist, H., Nuhkat, M., & Roelfsema, M. R. G. (2014) · New Phytologist · doi:10.1111/nph.12832 · Cited by 357
Metadata
- Authors
- Peiter, E., Maathuis, F. J. M., Mills, L. N., Knight, H., Pelloux, J., Hetherington, A. M., & Sanders, D.
- Venue
- Nature
- Published
- 2005-03
- Volume
- 434
- Issue
- 7031
- Pages
- 404-408
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2062422342
- Cited by
- 538
- References
- 24
- FWCI
- 33.65
- Type
- journal article
- Language
- en
- Open access
- closed
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- Peiter, E., Maathuis, F. J. M., Mills, L. N., Knight, H., Pelloux, J., Hetherington, A. M., & Sanders, D. (2005). The vacuolar Ca2+-activated channel TPC1 regulates germination and stomatal movement. Nature. https://doi.org/10.1038/nature03381
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