Functional genomics reveals genes involved in protein secretion and Golgi organization
Abstract
Yeast genetics and in vitro biochemical analysis have identified numerous genes involved in protein secretion. As compared with yeast, however, the metazoan secretory pathway is more complex and many mechanisms that regulate organization of the Golgi apparatus remain poorly characterized. We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. We then classified the genes on the basis of the effect of their depletion on organization of the Golgi membranes. Here we show that depletion of class A genes redistributes Golgi membranes into the endoplasmic reticulum, depletion of class B genes leads to Golgi fragmentation, depletion of class C genes leads to aggregation of Golgi membranes, and depletion of class D genes causes no obvious change. Of the 20 new gene products characterized so far, several localize to the Golgi membranes and the endoplasmic reticulum.
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Tango5 and Klp61F were recently identified as key regulators of Golgi apparatus structure and organization.
“Two modifiers identified from this study, Transport and Golgi organization 5 (Tango5) and Klp61F, were also recently identified as key regulators of Golgi apparatus (GA) structure and organization (229).”
From Functional genomic screen and network analysis reveal novel modifiers of tauopathy dissociated from tau phosphorylation (2011) · cites this paper as [229]
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- Authors
- Bard, F., Casano, L., Mallabiabarrena, A., Wallace, E., Saito, K., Kitayama, H., Guizzunti, G., Hu, Y., Wendler, F., DasGupta, R., Perrimon, N., & Malhotra, V.
- Affiliations
- University of California San Diego (US)
- National Institute for Medical Research (GB)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Malhotra, Vivek
- Venue
- Nature
- Published
- 2006-02
- Volume
- 439
- Issue
- 7076
- Pages
- 604-607
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2040495000
- Cited by
- 372
- References
- 0
- Citations by year
- 2026: 15 · 2025: 18 · 2024: 22 · 2023: 15 · 2022: 21 · 2021: 18 · 2020: 12 · 2019: 19 · 2018: 12 · 2017: 22 · 2016: 20 · 2015: 17 · 2014: 14 · 2013: 23 · 2012: 23
- FWCI
- 7.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- National Institutes of Health
- University of California, San Diego
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- protein secretion, secretory pathway, RNAi screen, Drosophila cells, Golgi fragmentation, endoplasmic reticulum, Golgi membrane proteins, gene function classification
- MeSH terms
- Animals, Cell Line, Drosophila, Endoplasmic Reticulum, Golgi Apparatus, Horseradish Peroxidase, Intracellular Membranes, Genes, Insect, Genes, Reporter, Protein Sorting Signals, Genomics, Drosophila Proteins, RNA Interference
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